Sentences with phrase «human adenoviruses in»

Second, our results show that PCR assays for human adenoviruses in common use are capable of detecting TMAdV.

Not exact matches

But the new test found another seven, including a respiratory virus called human adenovirus B type 3A, which usually is harmless but can cause severe infections in some patients.

«No data from humans vaccinated with adenovirus 5 vectors are described in this paper,» says one of them, Dan Barouch of the Beth Israel Deaconess Medical Center at Harvard Medical School.

Adenovirus 5 has 50 or so known relatives that infect humans and so in principle could also be used as a basis for vaccines, as could one from a chimpanzee.

Despite their popularity — several HIV vaccines are in development using human adenoviruses — the approach has a significant drawback.

The work skirts a problem that has cropped up in experimental vaccines made from a similar human adenovirus — they don't work if you've already been exposed to the natural virus.

But in mice that had been exposed to human adenovirus, only the chimp virus - based vaccine worked, the researchers report in the March issue of the Journal of Virology.

Chimp adenovirus vaccines may have an edge over those that use the human version, shown here in an electron micrograph (hexagon shapes).

Adenoviruses, first isolated in the 1950s from explanted adenoid tissue, are double - stranded nonenveloped DNA viruses that naturally infect many vertebrates, including humans and nonhuman primates.

To assess cross-neutralization of TMAdV by known human adenoviruses, 7 pools of in - house reference sera at the VRDL (rabbit hyperimmune sera) and collectively containing antibodies to human adenovirus serotypes 1 through 35 were available for testing.

However, the high sequence divergence in the fiber protein (Table 2), as well as the absence of fiber motifs conserved among adenoviruses that bind CAR [36], [37](coxsackievirus - adenovirus receptor) or CD46 [38], [39], [40](data not shown), suggest that neither of these two human adenoviral receptors may be the attachment receptor for TMAdV.

We used the Virochip, a microarray designed to detect all viruses, to identify a new species of adenovirus (TMAdV, or titi monkey adenovirus) that caused a deadly outbreak in a colony of New World titi monkeys at the California National Primate Research Center (CNPRC), and also infected a human researcher.

Adenoviruses are DNA viruses that naturally infect many vertebrates, including humans and monkeys, and cause a wide range of clinical illnesses in humans.

After removal of similar viral genomes, bootscan plots of the whole genome and individual genes from a subset representing human / simian adenoviruses in species A — G and all non-primate vertebrate adenoviruses were generated.

Human adenoviruses do not usually replicate in monkey cells in the absence of helper viruses [11], and do not productively infect rodents (and vice versa)[12].

Some serotypes, such as human adenovirus type 14 (HAdV - 14), have been associated with severe and potentially fatal outbreaks of pneumonia in residential facilities and military bases [4].

The fragment shared ∼ 86 % nucleotide identity with its closest phylogenetic relatives in GenBank, SAdV - 18, an Old World vervet monkey adenovirus, and the human species D adenoviruses.

In this regard, the high sequence divergence of TMAdV relative to the known human / simian adenoviruses (Fig. 3), and comparable sequence similarity in the polymerase gene to a porcine adenovirus (Figs. 3 and S1) are strikinIn this regard, the high sequence divergence of TMAdV relative to the known human / simian adenoviruses (Fig. 3), and comparable sequence similarity in the polymerase gene to a porcine adenovirus (Figs. 3 and S1) are strikinin the polymerase gene to a porcine adenovirus (Figs. 3 and S1) are striking.

Although sequencing of PCR amplicons for human adenoviruses is not performed routinely in diagnostic virology, TMAdV would presumably have been detected previously in large - scale studies of hexon sequencing of Ad field isolates if it were circulating in the community [46], [47].

Interestingly, pleurisy has been specifically reported in association with certain human adenovirus infections [44].

The decreased levels of neutralizing Abs to TMAdV in the researcher (1 ∶ 32) and a family member (1 ∶ 8) relative to those in infected titi monkeys (up to > 1 ∶ 512) are consistent with a recent study showing much higher levels of neutralizing antibodies in chimpanzees than in humans with adenovirus infections, possibly due to more robust adenovirus - specific T - cell responses in humans than in monkeys [45].

However, recent serological surveys have found antibodies to New World and Old World monkey adenoviruses in donor human sera from regions where the monkeys are endemic [14], [15].

The scanning nucleotide pairwise identities of TMAdV relative to representative human (yellow) or simian (brown) adenoviruses in species A — G, porcine adenovirus (red), and fowl adenovirus (green) are shown.

A defective adenovirus (one that can not grow or cause adenovirus infections in humans) is sometimes used as a vector in HIV vaccines.

Open Reading Frame E3 - 10.9 K of Subspecies B1 Human Adenoviruses Encodes a Family of Late Orthologous Proteins That Vary in Their Predicted Structural Features and Subcellular Localization.

Such antibodies are expected to reduce the efficacy of vaccines based on common human serotypes of adenovirus in a human target population.

These viruses do not circulate in the human population and fail to carry neutralizing B cell epitopes that cross-react with the common serotypes of human adenoviruses.

Posttranscriptional block to synthesis of a human adenovirus capsid protein in abortively infected monkey cells

Synthesis of human adenovirus early RNA species is similar in productive and abortive infections of monkey and human cells

Normal translation of human adenovirus mRNA in cell - free lysates prepared from abortively as well as productively infected monkey cells

Characterization of a variant of human adenovirus type 2 which multiples efficiently in simian cells

Characterization of the translational defect to fiber synthesis in monkey cells abortively infected with human adenovirus: Role of ancillary leaders

Scale - Up of the Adenovirus Expression System for the Production of Recombinant Protein in Human 293S Cells.

Partial block to transcription of human adenovirus type 2 late genes in abortively infected monkey cells

Both transient transfection with Lipofectamine and transduction with adenovirus resulted in a subpopulation of cells that experessed human IgG, as shown by the increased fluorescence intensity.

Improving the oncolytic potency of agents has been hampered by the inability to study host - vector interactions in immune - competent systems, since human serotype adenoviruses do not productively replicate in animal tissues.

In group two, eight children with unexplained fevers were tested; standard testing found 11 viruses, the new test found an additional seven, including the respiratory virus human adenovirus B type 3A.