Sentences with phrase «human breast cancer cell lines»

Ab7291 staining alpha tubulin in human breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).

Two metastatic human breast cancer cell lines (MDA - MB 231 and BCM2) were used to optimize the conditions of hypoxia and reoxygenation cycles.

The researchers observed the effect of the synthetically produced molecule, JK - 31, on the growth and proliferation of a model human breast cancer cell line and found that it effectively blocked the protein cyclin - dependent kinase 1 (CDK1), which plays a key part in the process of the division of cancer cells, and therefore inhibited the proliferation of the cells.

In this new study, described Feb. 21 in Cell Reports, Semenza and his colleagues conducted gene expression analysis of multiple human breast cancer cell lines grown in the laboratory after exposure to chemotherapy drugs, like carboplatin, which stops tumor growth by damaging cancer cell DNA.

Recent studies with human breast cancer cell lines have also shown that Shp2 mediates survival signals in cancer cells.

The researchers then injected AAV2 into human breast cancer cell line - derived tumors in mice without functioning immune systems.

Using an optimized hypoxia and reoxygenation regimen, we identified a novel cycling hypoxia - selected subpopulation from human breast cancer cell lines and demonstrated that a stem - like breast cancer cell subpopulation could be expanded through repetitive hypoxia / reoxygenation cycles without genetic manipulation.

To study the effect of hypoxia / reoxygenation cycles on breast cancer, we exposed two metastatic human breast cancer cell lines (MDA - MB 231 and BCM2) to cycles of chronic hypoxia and nutrient deprivation.

He is researching the functions and properties of human sulfotransferase (EST) enzymes in human breast cancer cell lines.

To see whether cancer stem cell renewal involves a chain of events similar to that used by embryonic stem cells, and whether the process was affected by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two human breast cancer cell lines that responded to low oxygen by ramping up production of the protein ALKBH5, which removes methyl groups from mRNAs.

The example shown reveals that these estrogen agonists show the strongest connectivity to each other in MCF7, a human breast cancer cell line that expresses the estrogen receptor.

The human breast cancer cell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotics.

To confirm specificity, supernatant from transfected or transduced HB1.F3 cells was incubated with MCF7, MCF7 / HER2, or BT474, a human breast cancer cell line that endogenously overexpresses HER2.

In collaboration with Nanyang Technological University, experiments were also conducted to co-deliver paclitaxel and small interfering RNA (siRNA) targeting a protein that prevents cell death (Bcl - 2) to MDA - MB - 231 human breast cancer cell line.

Gene amplification of MELK occurs in both primary tumors and human breast cancer cell lines, especially in ER - negative samples (Figure 3 — figure supplement 1).

We further confirmed that consistent with the results of the human breast cancer cell lines (Figure 2), the protein level of APC was elevated in the human breast cancer cells isolated from the anti-miR-142-3p-expressing breast cancer xenograft (Figure 7B).