Sentences with phrase «human breast cell lines»

Ortiz C, Morales L, Sastre M, Haskins WE, Matta J. Cytotoxicity and genotoxicity assessment of sandalwood essential oil in human breast cell lines MCF - 7 and MCF - 10A.

For the study, the researchers analyzed gene expression from human breast cell lines lacking HOXA5.

Not exact matches

Potti and his colleagues began by testing chemotherapy drugs on cultured cell lines from human tumors, such as from the lung, breast, or ovary.

The researchers observed the effect of the synthetically produced molecule, JK - 31, on the growth and proliferation of a model human breast cancer cell line and found that it effectively blocked the protein cyclin - dependent kinase 1 (CDK1), which plays a key part in the process of the division of cancer cells, and therefore inhibited the proliferation of the cells.

Beyond lung cancer, TiY is able to target TICs in 28 types of human cell lines derived from the central nervous system, melanoma, breast, renal, ovarian, colon, and prostate cancer.

To see whether cancer stem cell renewal involves a chain of events similar to that used by embryonic stem cells, and whether the process was affected by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two human breast cancer cell lines that responded to low oxygen by ramping up production of the protein ALKBH5, which removes methyl groups from mRNAs.

Through these effects, the PERY peptide reduced the proliferation of several (but not all) cancer cell lines in culture and inhibited the growth of a human breast cancer xenograft in mice.

In earlier studies involving animal models and human cancer cell lines, researchers found that breast cancer spreads when three specific cells are in direct contact: an endothelial cell (a type of cell that lines the blood vessels), a perivascular macrophage (a type of immune cell found near blood vessels), and a tumor cell that produces high levels of Mena, a protein that enhances a cancer cell's ability to spread.

The resulting «map» of gene - drug interactions allowed the researchers to accurately predict the responses of multiple human cancer cell lines to different chemotherapy agents based on the cell lines» genetic profiles and also revealed new genetic factors that appear to determine the response of breast and ovarian tumor cells to common classes of chemotherapy treatment.

He is researching the functions and properties of human sulfotransferase (EST) enzymes in human breast cancer cell lines.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

Recent studies with human breast cancer cell lines have also shown that Shp2 mediates survival signals in cancer cells.

The example shown reveals that these estrogen agonists show the strongest connectivity to each other in MCF7, a human breast cancer cell line that expresses the estrogen receptor.

The researchers then injected AAV2 into human breast cancer cell line - derived tumors in mice without functioning immune systems.

Here, we show that a non-adherent, stem - like, and metastatic CSC - enriched subpopulation could be isolated by exposing human metastatic breast cancer cell lines to cycles of chronic hypoxia followed by reoxygenation.

Using cutting - edge techniques enabled by next - generation sequencing, the authors generated complete methylome maps at single nucleotide resolution in a low - passage breast cancer cell line and normal breast tissue (primary human mammary epithelial cells).

To study the effect of hypoxia / reoxygenation cycles on breast cancer, we exposed two metastatic human breast cancer cell lines (MDA - MB 231 and BCM2) to cycles of chronic hypoxia and nutrient deprivation.

Ab7291 staining alpha tubulin in human breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).

Conditions for hypoxia / reoxygenation cycles were optimized using two human metastatic breast cancer cell lines (MDA - MB 231 and BCM2).

The human breast cancer cell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotcell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotCell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotics.

Flavopiridol inhibits several cellular kinases and has demonstrated cytostatic and cytotoxic activity in vitro and in vivo in numerous human tumor cell lines and xenograft models (including human breast, prostate, and lung carcinoma) at clinically achievable concentrations.

Cultures of the human triple negative breast cancer cell line, HCC1806, were maintained in RPMI 1640 medium supplemented with 10 % FBS.

In collaboration with Nanyang Technological University, experiments were also conducted to co-deliver paclitaxel and small interfering RNA (siRNA) targeting a protein that prevents cell death (Bcl - 2) to MDA - MB - 231 human breast cancer cell line.

Recent reports show increased CSC activity following hypoxic exposure in breast cancer cell lines (18 — 20), but no reports have studied this rare population in primary human breast cancer samples.

To confirm specificity, supernatant from transfected or transduced HB1.F3 cells was incubated with MCF7, MCF7 / HER2, or BT474, a human breast cancer cell line that endogenously overexpresses HER2.

It can efficiently introduce DNA into a cell to be incorporated into its genetic make - up, i.e. induce high gene expression level, especially in both human and mouse breast cancer cell lines, and mouse breast cancer model.

ab1 staining HIF -1-alpha in MCF7 (Human breast adenocarcinoma cell line) cells treated with metformin hydrochloride ab12084, by ICC / IF.

Dissertation: «Epigenetic Modification of Vitamin D - induced Gene Expression in Human Colorectal and Breast Cancer Cell Lines.»

The curcumin metabolite tetrahydrocurcumin decreases the activity of the phase III transporters in human cervical carcinoma and breast cancer cell lines.

The Journal of Agricultural and Food Chemistry had on its December 2006 issue a report that found the ability of red raspberries to repress the human oral and breast, as well as colon and prostate tumour cell lines growths when they were tested in test tubes.

Bottom line While research has shown some benefits with regards to ER + breast cancer cell death and prevention of metastases within mice and cellular models, it is recommended that human intake should be through diet only, not supplementation.

A recent study out of Georgetown University showed that the metals mercury, copper, cobalt, nickel, lead, tin, chromium and vanadate activated estrogen receptor alpha sites in human breast cell cancer lines in ways similarly to the human estrogen estradiol.