Sentences with phrase «human cell line»

2fTGH is a human cell line containing the selectable marker guanine phosphoribosyltransferase regulated by alpha interferon (IFN - alpha).

Defined human cell line cores containing positive and negative protein expressing cell lines on the same slide

Now Carette and co-workers have used a similar approach and used a human cell line, in which nearly all human chromosomes are present in a single copy.

Overall, making a human cell line with a gene knockout was straightforward, and is a testament to how far genome engineering has come.

IHC - P mouse tumor tissue (from lung) with human cell line injected, some muscle tissue attached as well sees high background for human cellswith priamry Ab as well as isotype ctrl, but also for muscle (does not contain any EGF) Ab: 1 ug /...

Thus, high ALKBH1 activity can globally decrease the amount of proteins made in a cell, as the researchers observed in a human cell line with abnormally high levels of ALKBH1.

Since their isolation in 1951, HeLa cells have been the most widely used human cell line in research.

Fourteen participating teams each engineered two chemotaxis models, the amoeba Dictyostelium discoideum (Dicty), and the human cell line HL60, to run a millimeter - long maze of interconnected, orthogonal channels designed to mimic the cell's natural environment.

Coffin described how lab workers there had transplanted human prostate tumor cells into an immune - deficient lab mouse, a common procedure for procuring a colony of cells, or a human cell line, for further study.

Professor Ali Tavassoli, who led the study with colleague Dr. Ishna Mistry, explains: «In an effort to better understand the role of HIF - 1 in cancer, and to demonstrate the potential for inhibiting this protein in cancer therapy, we engineered a human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environment.

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

Capsaicin additionally produced a significant deceleration of the development of prostate tumors created simply by those human cell lines grown in mouse models.

The work, funded by the US National Human Genome Research Institute, aims to create human cell lines with subtly different genomes in order to test ideas about which mutations cause disease and how.

Her group will focus on the biogenesis and homeostasis of mitochondrial proteins and add mammalian and human cell lines to current yeasts.

Beyond lung cancer, TiY is able to target TICs in 28 types of human cell lines derived from the central nervous system, melanoma, breast, renal, ovarian, colon, and prostate cancer.

The scientists used the new indexing method on several human cell lines and from a mouse brain to reveal the methylome of 3,282 single cells.

To this end, they applied Paroxetine to human cell lines for three weeks — the time it takes for a clinical response to SSRIs.

During the last several years, research efforts with human cell lines revealed that the genomic loci encoding tRNAs give rise to shorter tRNA fragments («tRFs»).

«We are now investigating chromosome fragile site formation in various human cell lines, including the chronic myeloid leukemia and Fragile X cells,» said Feng.

HeLa cells are the most widely used human cell lines in existence today.

All human cell lines growing in tissue culture dishes, and about 95 % of cells in a human being, split evenly, resulting in daughter cells of equal size.

They tried hundreds of different recipes; eventually they discovered that if they mixed liver precursor cells (derived from iPS cells) with two other types of standard human cell lines known to be important for embryonic liver development, then the cells would spontaneously form a 4 to 5 - millimeter 3D structure called a liver bud.

Our investigations on human cell lines in the laboratory have shown that synthetic cannabinoids, in the high concentrations found in cells in the oral cavity or in the lungs, for example, are likely to trigger damage to the DNA that may have significant consequences for the consumers of such substances.

Kim, Jung - Sik, et al. «Epitope tagging of endogenous genes in diverse human cell lines.»

Experimental models include yeast, mammalian and human cell lines, mutant mouse models and tumour samples.

Until now, scientists examining the causes and effects of insulin resistance have struggled with a general lack of human cell lines from tissues such as muscle, fat and liver that respond significantly to insulin, Kahn says.

TNA3: BPRC will offer access to its library of human cell reporter assays for the identification of innate immune receptor - induced signalling cascades, including human cell lines transfected with PAMPs such as TLR and / or engineered to express luciferase in response to NF - kB or IFRE - mediated signal transduction, and cell lines that provide insight into the intracellular signalling cascades of innate immune receptors.

It also works in cells taken directly from human patients, which are usually much more difficult to manipulate than human cell lines grown specifically for lab research.

Manandhar M, Lowery MG, Boulware KS, Lin KH, Lu Y, Wood RD. (2017) Transcriptional consequences of XPA disruption in human cell lines.

The first version of Project Discovery will include classification of patterns of protein subcellular distribution in human cell lines.

In addition to our computational work, we run a small wetlab where we use CRISPR - Cas genome editing in human cell lines to obtain a deeper understanding of how genetic variants affect the cell.

In MMEJ pathway, we achieve efficient gene disruption in human cell lines and animals by developing a computer program that assists the choice of nuclease target sites based on microhomology prediction.

With the new tool, researchers uncovered the location of «i - motifs» in a range of human cell lines.

University of Wisconsin A chemical - genetic library of human cell lines for evaluating pharmacologic inhibition of protein kinases

In addition to the human cell lines, the mouse cell line NIH 3T3 is stained.

Extensive use is made of our human brain bank and our stock of human cell lines.

Based on the resource of more than 47,000 antibodies and a panel of > 20 different human cell lines, this facility has a unique position to investigate subcellular spatial proteomics for human and rodent biology.

I have 2 abcam antibodies (ab1 and ab16066) and i am trying to blot for bovine, rat, pig, and human cell lines and tissues.

BPRC will offer access to its library of human cell reporter assays for the identification of innate immune receptor - induced signalling cascades, including human cell lines transfected with PAMPs such as TLR and / or engineered to express luciferase in response to NF - kB or IFRE - mediated signal transduction, and cell lines that provide insight into the intracellular signalling cascades of innate immune receptors.

The organizers of the project, called GP - write (for work in model organisms and plants) or sometimes HGP - write (for work in human cell lines), envision it as a successor to the Human Genome Project (retroactively termed HGP - read), which 25 years ago promoted rapid advances in DNA sequencing technology.

Drug ‐ induced transcriptional modules (biclusters) were identified and annotated in three human cell lines and rat liver.

Glyphosate - based herbicides are toxic and endocrine disruptors in human cell lines.

Gasnier, C.; Dumont, C.; Benachour, N.; Clair, E.; Chagnon, M.C.; Séralini, G.E. Glyphosate - based herbicides are toxic and endocrine disruptors in human cell lines.

Recent studies using animal and human cell lines and knockout mice have shown that low - energy sweeteners can stimulate intestinal enteroendocrine cells to release glucagon - like peptide - 1 and glucose - dependent insulinotropic peptide.

Not exact matches

The National Institutes of Health (NIH) has allowed a contract for the dissemination of embryonic stem cell lines approved for US government funding to lapse, shuttering a key stem cell bank, and doubling the price researchers pay for samples of some human embryonic stem cell (hESC) line... from $ 500 per vial of frozen cells to $ 1,000.

In August of last year, President Bush approved the use of federal funds to support research on a limited number of existing human embryonic stem cell lines.

Modern psychosomatic medicine has made some progress in analyzing along these lines; for example, it seems quite possible that the emotional tone of my soul may directly alter the patterns of physical feeling in my stomach.4 Still, we should not suppose too quickly that the aims of a human personality have any very effective direct influence on the molecules of body cells, other than those in the brain.

Although he never banned this research outright, President Bush limited federal funding for research to the embryonic stem cell lines that existed before August 2001, thus drawing a line at destroying human embryos created after that date.

Cholesterol is carried in the blood and is infused into the cells lining the blood vessels, says Prof. Seneviratne dealing with the chemistry of the human body.

«Brain scientists have invented, established and patented a method to obtain long - living cell lines derived from fungiform taste papillae of the human tongue.