Sentences with phrase «human cell lines»

Extensive use is made of our human brain bank and our stock of human cell lines.

It also works in cells taken directly from human patients, which are usually much more difficult to manipulate than human cell lines grown specifically for lab research.

In the rare human cell line used for this genetic screen, the cells have only one copy of each chromosome, except for chromosome 8, which has two copies.

To verify that the small change could have a noticeable effect, the researchers examined IL - 12 production in human cell lines with and without the altered base pair.

The work, funded by the US National Human Genome Research Institute, aims to create human cell lines with subtly different genomes in order to test ideas about which mutations cause disease and how.

Since their isolation in 1951, HeLa cells have been the most widely used human cell line in research.

Our investigations on human cell lines in the laboratory have shown that synthetic cannabinoids, in the high concentrations found in cells in the oral cavity or in the lungs, for example, are likely to trigger damage to the DNA that may have significant consequences for the consumers of such substances.

It is gratifying that Justice Mrs. Dobbs recognized that the science behind the creation of hybrid embryos was always about creating unique cloned human cell lines that could accelerate the development of therapies for a number of important neurodegenerative conditions.

The scientists scanned three different human cell lines for ERVs in their DNA that could bind to innate immunity transcription factors, which turn on genes to ramp up the immune system's attack against pathogens.

University of Wisconsin A chemical - genetic library of human cell lines for evaluating pharmacologic inhibition of protein kinases

Her cancer cells, kept alive in the lab, became the first immortal human cell line, growing and dividing indefinitely.

Schweppe started searching the American Tissue Culture Collection (ATCC) database of nearly 1,200 fingerprinted human cell lines, both normal and malignant, from tissues, organs and tumors.

Beyond lung cancer, TiY is able to target TICs in 28 types of human cell lines derived from the central nervous system, melanoma, breast, renal, ovarian, colon, and prostate cancer.

The scientists used the new indexing method on several human cell lines and from a mouse brain to reveal the methylome of 3,282 single cells.

During the last several years, research efforts with human cell lines revealed that the genomic loci encoding tRNAs give rise to shorter tRNA fragments («tRFs»).

«We are now investigating chromosome fragile site formation in various human cell lines, including the chronic myeloid leukemia and Fragile X cells,» said Feng.

They tried hundreds of different recipes; eventually they discovered that if they mixed liver precursor cells (derived from iPS cells) with two other types of standard human cell lines known to be important for embryonic liver development, then the cells would spontaneously form a 4 to 5 - millimeter 3D structure called a liver bud.

Kim, Jung - Sik, et al. «Epitope tagging of endogenous genes in diverse human cell lines.»

IHC - P mouse tumor tissue (from lung) with human cell line injected, some muscle tissue attached as well sees high background for human cellswith priamry Ab as well as isotype ctrl, but also for muscle (does not contain any EGF) Ab: 1 ug /...

Defined human cell line cores containing positive and negative protein expressing cell lines on the same slide

Construction of human cell lines which contain and express the adenovirus DNA binding protein gene by cotransformation with the HSV - 1 tk gene

It was also tested using the human gastric epithelial cell line GES - 1 (more here on how human cell lines are commonly used in research) which was incubated with GABA and then treated with ethanol to induce cell injury.

HeLa cells are the most widely used human cell lines in existence today.

Based on the resource of more than 47,000 antibodies and a panel of > 20 different human cell lines, this facility has a unique position to investigate subcellular spatial proteomics for human and rodent biology.

Manandhar M, Lowery MG, Boulware KS, Lin KH, Lu Y, Wood RD. (2017) Transcriptional consequences of XPA disruption in human cell lines.

To this end, they applied Paroxetine to human cell lines for three weeks — the time it takes for a clinical response to SSRIs.

Effect of predatory bacteria on human cell lines.

Until now, scientists examining the causes and effects of insulin resistance have struggled with a general lack of human cell lines from tissues such as muscle, fat and liver that respond significantly to insulin, Kahn says.

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

Capsaicin additionally produced a significant deceleration of the development of prostate tumors created simply by those human cell lines grown in mouse models.

The UAB team next showed that knocking out YME1L1 in a human cell line strikingly increased the amount of mitochondrial DNA in the nuclear fraction of YME1L1 knockout cells.

The study tested phytic acid in an Alzheimer's mouse model and in a human cell line.

Professor Ali Tavassoli, who led the study with colleague Dr. Ishna Mistry, explains: «In an effort to better understand the role of HIF - 1 in cancer, and to demonstrate the potential for inhibiting this protein in cancer therapy, we engineered a human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environment.

The genetic circuit is incorporated onto the chromosome of a human cell line, which encodes the protein machinery required for the production of their cyclic peptide HIF - 1 inhibitor.

Her group will focus on the biogenesis and homeostasis of mitochondrial proteins and add mammalian and human cell lines to current yeasts.

One human cell line they studied displays the receptors for estrogen and progesterone, and one, known as triple negative, displays none.)

Coffin described how lab workers there had transplanted human prostate tumor cells into an immune - deficient lab mouse, a common procedure for procuring a colony of cells, or a human cell line, for further study.

Fourteen participating teams each engineered two chemotaxis models, the amoeba Dictyostelium discoideum (Dicty), and the human cell line HL60, to run a millimeter - long maze of interconnected, orthogonal channels designed to mimic the cell's natural environment.

The researchers first tested 142 natural compounds on mouse and human cell lines to see which inhibited prostate cancer cell growth when administered alone or in combination with another nutrient.

In the new work, carried out in a human cell line, they found that two elements, a protein called Kir4.2 (made by gene KCNJ15) and molecules within the cell called polyamines, were needed for signaling to occur.

One set of examples involves «ultrasafe» human cell lines: Cells used to secrete proteins used in drug treatments could be engineered to be resistant to viruses that might contaminate the product.

Zhao and colleagues have been studying these «electric senses» in cells from both larger animals (fish skin cells, human cell lines) and in the soil - dwelling amoeba Dictyostelium.

All human cell lines growing in tissue culture dishes, and about 95 % of cells in a human being, split evenly, resulting in daughter cells of equal size.

Okairos, a small company that produces the GSK vaccine at a facility near Rome,, grows its virus in a human cell line in up to 200 - liter wave bags, so - called because they rock back and forth on a platform.

In the new work in BioMed Research International, Séralini and three co-authors affiliated with the University of Caen and a private organization founded by Séralini, the Committee for Research and Independent Information on Genetic Engineering, examine the effects of nine pesticides on three human cell lines.

In particular, this research showed that one lncRNA, called LINC01116, is upregulated in a human cell line of prostate cancer, but can be decreased by treatment with sulforaphane.

She said genetic tests on the cell line described in Hwang's 2004 paper in Science, purportedly describing creation of the first — and so far the only — human cell line cloned from a healthy oocyte donor, are ongoing.

Thus, high ALKBH1 activity can globally decrease the amount of proteins made in a cell, as the researchers observed in a human cell line with abnormally high levels of ALKBH1.