Sentences with phrase «human cell lines derived»

Beyond lung cancer, TiY is able to target TICs in 28 types of human cell lines derived from the central nervous system, melanoma, breast, renal, ovarian, colon, and prostate cancer.

Not exact matches

«Brain scientists have invented, established and patented a method to obtain long - living cell lines derived from fungiform taste papillae of the human tongue.

Before we established in - house human taste cell (HTC) technologies, it was not possible to use human tongue - derived cells as a model, due to the lack of homogenous, proliferating cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.

Where permitted, ES cells should be drawn from sources in the following order: (1) existing ES cell lines, originating from ES cells derived from embryos less than 14 days old; and (2) surplus human embryos less than 14 days old that were created for fertility treatment.

Another problem is that in its July 2009 Guidelines on Human Stem Cell Research, NIH spelled out specific requirements about embryo donation for newly derived lines, says Pilar Ossorio, a legal scholar who studies research ethics at the University of Wisconsin Law School.

They found out that TiY is capable of distinguishing TICs from non-TICs in various human lung cancer cell lines and patient - derived lung tumors.

In an advance touted as a way around current political logjams, scientists have said they can derive human embryonic stem (ES) cell lines without destroying an embryo.

Proponents of ESC research counter that most of the new cell lines could be derived from donated, unused human embryos created by couples seeking fertility treatment, and that more than a third of zygotes fail to implant after conception, so those would be lost by chance anyway.

Researchers working to understand how to manipulate this powerful tool get their supply from stem cell lines, groups of stem cells derived from animal or human tissue that can reproduce themselves indefinitely.

In addition to the afatinib - resistant NSCLC cells, the researchers tested the neratinib and valproic acid combination on cell lines derived from human pancreatic and ovarian cancers containing K - Ras mutations and N - Ras mutations, respectively.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gcell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gCell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

This rhinovirus reliably kills HeLa cells, a line of human - derived cells that have long been used in studies of viral infection.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channel.

In February 2004 Hwang and his research group reported the first embryonic stem cell line derived from a cloned human embryo.

The researchers tested honokiol on cell lines derived from human cancers of the oral cavity, larynx, tongue, and pharynx.

«We set out to test this in cell lines derived from mice and humans, and came up with a result we definitely did not expect — which then led us to dig deeper for answers.»

They tried hundreds of different recipes; eventually they discovered that if they mixed liver precursor cells (derived from iPS cells) with two other types of standard human cell lines known to be important for embryonic liver development, then the cells would spontaneously form a 4 to 5 - millimeter 3D structure called a liver bud.

In four cell lines derived from human and monkey, siRNAs substantially lowered the expression of three of four test genes — by as much as 90 %.

Neural stem cell lines derived from human fetal brain tissue achieved the same feats, the researchers report in the October issue of Nature Neuroscience.

Attempting to find middle ground in the contentious debate over human embryonic stem cells, President George W. Bush announced 9 August that the National Institutes of Health (NIH) will be allowed to fund work with embryonic stem (ES) cell lines — but only those lines that have already been derived.

Since last year, teams in Italy, Scotland, and the United States have reported deriving ES cell lines from human parthenotes, although none of the lines is well studied.

In the PNAS paper, the scientists demonstrated their method on two genomics problems, the role of gene enhancers in the fruit fly embryo and alternative splicing in a human - derived cell line.

Palanichamy, Chakravarti and their colleagues conducted this study using 13 primary GBM cell lines derived from patient tumors, four commercially available GBM cell lines and normal human astrocyte cells.

We assayed from 6000 to 20,000 shRNAs simultaneously to identify genes important for the proliferation and survival of five cell lines derived from human mammary tissue.

«The use of nonhuman oocytes for SCNT is currently the only ethically justifiable option given the large numbers of eggs required to derive cloned human stem cell lines,» he said.

Nonetheless, passage of the measure marks a significant milestone in efforts to overturn the Bush policy, which limits government funding to research using 60 human ES cell lines derived before 9 August, 2001.

Though Hwang Woo - suk, a South Korean scientist claimed to have created the first human embryonic clone and derived a stem - cell line from it in 2004 his work was later shown to be fraudulent.

Twenty - seven human embryonic stem cell (hESC) lines are ethically derived and should be approved for use in research funded by the US linkurl: National Institutes of Health; http://www.nih.gov/ (NIH), a committee advising the NIH director linkurl: recommended today (December 4).

Only a few laboratories in the world are attempting this technique in human stem cell research and, thus far, no human stem cell lines have been derived using this method.

Novel cell lines derived from adult human ventricular cardiomyocytes.

NeuroStemcell is focused on the identification and systematic comparison of progenitor cell lines with the most favourable characteristics for mesDA and striatal GABAergic neuronal differentiation, generated either directly from human embryonic stem (ES) cells, from Neural Stem (NS) cells derived from ES cells or fetal brain, from induced Pluripotent Stem (iPS) cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate cells in appropriate animals models.

NCI's efforts to develop new laboratory models of human cancer includes vastly increasing the number of human cancer cell lines (grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.

HAT sensitive derivative of CEM, a human T - cell line derived from the peripheral blood buffy coat of a four - year old Caucasian female with acute lymphoblastic leaukemia.

«Finally, to assess the potential fate of mtDNA under condition when the cells can replicate extensively (longer than allowed by UK law), they derived human embryonic stem cell (hESC) lines from five ePNT blastocysts.

Using pre-implantation genetic diagnosis derived human embryonic stem cell lines (hES) or human induced pluripotent stem cells carrying the causal mutation for neuro - muscular diseases, our objective is double: identify new physiopathogical mechanisms and develop new therapeutic strategies.

Eliminating these constraints, hepatocytes differentiated from human induced pluripotent stem (hiPS) cells provide an unlimited supply of functional hepatocytes from each parental cell line, which can be derived noninvasively from a diseased or healthy person.

The researchers then injected AAV2 into human breast cancer cell line - derived tumors in mice without functioning immune systems.

Subclone of A3.01, a CD4 + CEM derived human T - cell line.

ScienceDaily «A human stem cell line derived from embryos that were identified by preimplantation genetic diagnosis (PGD) to carry the mutation for fragile X syndrome has...

HuH - 7 (human HCC), HepG2 (human hepatocellular blastoma), 293T (human embryonic kidney fibroblast), HeLa (human cervical cancer cells), NIH 3T3 (murine fibroblast), and HCT116 (human colon cancer) and its derived cell line HCT116 p21 − / − (kindly provided by Dr. B. Vogelstein, Howard Hughes Medical Institute, Chevy Chase and the Sydney Kimmel Comprehensive Cancer Center of the Johns Hopkins University School of Medicine, Baltimore, MD; ref.

Labs that derive new human embryonic stem cell lines are few, partly because they can not get financial support from federal sources.

The UC06 (HSF6) human ES cell line (P62) was obtained from the National Stem Cell Bank (NSCB), and the adipose derived mesenchymal stem cell (AD - MSC) line was generously obtained from Dr. Jeffrey Gimble from the Penington Biomedical Research Institcell line (P62) was obtained from the National Stem Cell Bank (NSCB), and the adipose derived mesenchymal stem cell (AD - MSC) line was generously obtained from Dr. Jeffrey Gimble from the Penington Biomedical Research InstitCell Bank (NSCB), and the adipose derived mesenchymal stem cell (AD - MSC) line was generously obtained from Dr. Jeffrey Gimble from the Penington Biomedical Research Institcell (AD - MSC) line was generously obtained from Dr. Jeffrey Gimble from the Penington Biomedical Research Institute.

To determine DAXX binding sites in the PCa genome, specifically in the PC3 cell line (derived from bone metastasis of a human PCa case [19]-RRB-, we generated a stable shRNA knockdown (K / D) PC3 cell line and a control shRNA counterpart [Submitted], and performed a ChIP - Seq study.

Thus, all research on lines of human embryonic stem cells as the cells of various phenotypes derived from these lines should aim to lexploration mechanisms or the development and validation of therapeutic applicable to serious diseases.

However, some consider human embryonic stem cell research controversial because, in some cases, the new stem cell lines are derived frozen human embryos that have been donated for research.

A glomerulus - on - a-chip lined by human stem cell - derived kidney cells could help model patient - specific kidney diseases and guide therapeutic discovery

The regulations for working with human materials, i.e. DNA, RNA, tissue and human - derived cell - lines, can be complex.

Various cell types have been examined for use in RPE cell replacement including immortalized cell lines, such as the human RPE cell line, ARPE19 [2], sheets of adult RPE [3], foetal RPE [4], RPE derived from human embryonic stem cells (HESC - RPE)[5]--[10] and many non-RPE cells lines [11]--[15].

The authors transplanted human cortical ‐ derived neural progenitor cells engineered to secrete glial cell line ‐ derived neurotrophic factor (GDNF) into the cortex of a rat model of ALS, where the cells migrated, matured into astrocytes, and released GDNF.