Sentences with phrase «human cell lines for»

University of Wisconsin A chemical - genetic library of human cell lines for evaluating pharmacologic inhibition of protein kinases

To this end, they applied Paroxetine to human cell lines for three weeks — the time it takes for a clinical response to SSRIs.

Not exact matches

The National Institutes of Health (NIH) has allowed a contract for the dissemination of embryonic stem cell lines approved for US government funding to lapse, shuttering a key stem cell bank, and doubling the price researchers pay for samples of some human embryonic stem cell (hESC) line... from $ 500 per vial of frozen cells to $ 1,000.

Modern psychosomatic medicine has made some progress in analyzing along these lines; for example, it seems quite possible that the emotional tone of my soul may directly alter the patterns of physical feeling in my stomach.4 Still, we should not suppose too quickly that the aims of a human personality have any very effective direct influence on the molecules of body cells, other than those in the brain.

Although he never banned this research outright, President Bush limited federal funding for research to the embryonic stem cell lines that existed before August 2001, thus drawing a line at destroying human embryos created after that date.

As well as allowing the use of stem cells grown from established cell lines, the technology could enable the creation of improved human tissue models for drug testing and potentially even purpose - built replacement organs.

Introducing human prostate cancer cell lines into mice, Wu and his colleagues saw a particular enzyme called MAOA activate a cascade of signals that made it easier for tumor cells to invade and grow in bone.

That summer, President George W. Bush had approved federal funding for human ES - cell research, but only for a small number of cell lines that had already been created.

Although the researchers emphasized that laboratory results involving cell lines and mice do not necessarily translate to human treatment, they say their findings show that new mTOR inhibitors combined with chemotherapy could become a new treatment strategy for T - ALL.

Where permitted, ES cells should be drawn from sources in the following order: (1) existing ES cell lines, originating from ES cells derived from embryos less than 14 days old; and (2) surplus human embryos less than 14 days old that were created for fertility treatment.

Church is already adapting MAGE for genetically modifying human stem cell lines.

Another problem is that in its July 2009 Guidelines on Human Stem Cell Research, NIH spelled out specific requirements about embryo donation for newly derived lines, says Pilar Ossorio, a legal scholar who studies research ethics at the University of Wisconsin Law School.

Professor Ali Tavassoli, who led the study with colleague Dr. Ishna Mistry, explains: «In an effort to better understand the role of HIF - 1 in cancer, and to demonstrate the potential for inhibiting this protein in cancer therapy, we engineered a human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environment.

The paper not only seemed to validate the group's claim a year earlier that it had created a single cell line from a cloned human embryo, but it also reported a huge increase in efficiency for the technique.

The laboratory process, described in the journal Scientific Reports, entails genetically modifying a line of human embryonic stem cells to become fluorescent upon their differentiation to retinal ganglion cells, and then using that cell line for development of new differentiation methods and characterization of the resulting cells.

Coffin described how lab workers there had transplanted human prostate tumor cells into an immune - deficient lab mouse, a common procedure for procuring a colony of cells, or a human cell line, for further study.

«This is an important and fundamental paper for understanding human germ - line cells and finding the basic information about human germ - cell biology,» says reproductive biologist Evelyn Telfer of the University of Edinburgh, UK.

MERS CoV (CoV stands for coronavirus) has on its surface an array of spike - shaped proteins that bind to host cells — specifically to receptor proteins called DPP4 on the surface of cells that line human airways.

Stem cell advocates have been expressing serious worry that ethical requirements spelled out in the draft guidelines — in particular, informed consent procedures for embryo donors — will rule out the use of many existing human embryonic stem cell lines, including the 21 lines approved under the Bush Administration.

The final guidelines on research with human embryonic stem cells issued on Monday by the National Institutes of Health set out criteria for determining which ES cell lines can be used in federally funded experiments and give NIH discretion to approve old lines that don't meet stringent modern ethical requirements.

The bill was put forth to loosen the restrictions Bush placed on human embryonic stem cell research on August 9, 2001, when he banned federal funding for work with any stem cell line created after that date.

While conservatives in Congress took turns echoing George W. Bush's opposition to destroying human embryos for research, Lensch's colleague Paul Lerou stepped into a small room behind a heavy black curtain to check up on a line of nonpresidential embryonic stem cells.

These human cells were ineligible for federal research funds because they had been created after President Bush's August 9, 2001, announcement freezing the number of government - approved stem cell lines.

The finding potentially paves the way for scores of labs to generate new stem cell lines without cloned embryos, which had long been considered the only realistic way of making human stem cells in the short run.

To test their hypothesis, the researchers carried out experiments on human melanoma cells, a line of cancer cells they chose for their ability to grow easily and quickly.

Human tumor tissue or cell lines can be coengrafted into these mouse models, providing a powerful tool for studying the interactions between human immune cells and human canHuman tumor tissue or cell lines can be coengrafted into these mouse models, providing a powerful tool for studying the interactions between human immune cells and human canhuman immune cells and human canhuman cancers.

The researchers hope their new cell lines will be a useful resource for studying the cellular and molecular intricacies of Huntington's further, and suggest they may provide a model for examining other diseases of the brain that are specific to humans.

For the study, the researchers analyzed gene expression from human breast cell lines lacking HOXA5.

Although Kato called human reproductive cloning directly from iPS cell lines «very hypothetical,» he pointed out progress for that possibility when he noted that three teams had produced mouse clones from iPS cells.

Furthermore, stem - cell lines from mice begin to lose their ability to produce many types of cells after as few as 20 generations, and researchers assume the same will prove true for stem cells from humans.

Salk scientists and colleagues have proposed new molecular criteria for judging just how close any line of laboratory - generated stem cells comes to mimicking embryonic cells seen in the very earliest stages of human development, known as naïve stem cells.

Obama and Clinton both voted for the Stem Cell Research Enhancement Act, which would have broadly extended federal funding to human embryonic stem cell lines but was vetoed by Bush in 2006 and 2Cell Research Enhancement Act, which would have broadly extended federal funding to human embryonic stem cell lines but was vetoed by Bush in 2006 and 2cell lines but was vetoed by Bush in 2006 and 2007.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channel.

The high quality of the new lines should make them appealing for clinical trials, says Braude, but it could take several years before the cells make their way into humans — if they ever do.

«We set out to test this in cell lines derived from mice and humans, and came up with a result we definitely did not expect — which then led us to dig deeper for answers.»

They tried hundreds of different recipes; eventually they discovered that if they mixed liver precursor cells (derived from iPS cells) with two other types of standard human cell lines known to be important for embryonic liver development, then the cells would spontaneously form a 4 to 5 - millimeter 3D structure called a liver bud.

For this reason, the National Cancer Institute maintains a library of 60 authenticated human cancer cell lines for the purposes of research, called the NCI - For this reason, the National Cancer Institute maintains a library of 60 authenticated human cancer cell lines for the purposes of research, called the NCI - for the purposes of research, called the NCI - 60.

Our investigations on human cell lines in the laboratory have shown that synthetic cannabinoids, in the high concentrations found in cells in the oral cavity or in the lungs, for example, are likely to trigger damage to the DNA that may have significant consequences for the consumers of such substances.

A bill that would allow federal funding for derivation of new lines of human embryonic stem (ES) cells passed the U.S. House of Representatives yesterday 238 to 194 after more than four hours of debate.

But a computer game that puts those kids on the front line of a battlefield inside the human body — where cancer cells and chemotherapy drugs are slogging it out for supremacy — just might persuade them that it's important to keep taking the drugs.

We assayed from 6000 to 20,000 shRNAs simultaneously to identify genes important for the proliferation and survival of five cell lines derived from human mammary tissue.

«The use of nonhuman oocytes for SCNT is currently the only ethically justifiable option given the large numbers of eggs required to derive cloned human stem cell lines,» he said.

However, Professor Auguste's team, discovered the overexpression of intercellular adhesion molecule - 1 (ICAM - 1) in human TNBC cell lines and tissues, and demonstrated that it is a potential molecular target and biomarker for TNBC therapy and diagnosis.

To overcome this, researchers from the University of Texas Southwestern Medical Center and Johns Hopkins University stabilized pri - miRNAs by expressing a dominant negative form of DROSHA, the RNase III enzyme responsible for pri - miRNA cleavage, in a variety of human and mouse cell lines.

For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectiveFor the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectivefor 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.

Twenty - seven human embryonic stem cell (hESC) lines are ethically derived and should be approved for use in research funded by the US linkurl: National Institutes of Health; http://www.nih.gov/ (NIH), a committee advising the NIH director linkurl: recommended today (December 4).

For while the new NIH guidelines explicitly permit funding for research on stem cell lines in which human embryos have already been destroyed, they also explicitly forbid funding for research on stem cell lines that have been produced by SCNT (see section V. part For while the new NIH guidelines explicitly permit funding for research on stem cell lines in which human embryos have already been destroyed, they also explicitly forbid funding for research on stem cell lines that have been produced by SCNT (see section V. part for research on stem cell lines in which human embryos have already been destroyed, they also explicitly forbid funding for research on stem cell lines that have been produced by SCNT (see section V. part for research on stem cell lines that have been produced by SCNT (see section V. part B).

However, we have found a fluorescent marker for nestin, a neural crest stem cell marker, in the posterior limbal region of the human anterior segment in the vicinity of both the trabecular meshwork and Schwalbe's line (unpublished).

What Collins does not say, however, is that the new NIH guidelines also allow for federal funds to be used in studying new human embryonic stem cell lines that are created (by private entities, of course) beyond the 700 currently in existence.

The new NIH guidelines do not permit the use of federal funds for creating new human embryonic stem cell lines.