Sentences with phrase «human cell lines which»

Not exact matches

Before we established in - house human taste cell (HTC) technologies, it was not possible to use human tongue - derived cells as a model, due to the lack of homogenous, proliferating cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.

To create the effect of tobacco smoke on cells, Vaz, Baylin and their colleagues began their studies with human bronchial cells, which line the airways of the lungs, and grew them in a laboratory.

The work, funded by the US National Human Genome Research Institute, aims to create human cell lines with subtly different genomes in order to test ideas about which mutations cause disease andHuman Genome Research Institute, aims to create human cell lines with subtly different genomes in order to test ideas about which mutations cause disease andhuman cell lines with subtly different genomes in order to test ideas about which mutations cause disease and how.

As those cells proliferated in laboratory dishes, the bits of human DNA were also copied, creating cell lines, each of which had a different fragment.

In experiments conducted on human lung endothelial cells and in mice, the researchers showed that NS1 caused permeability of the endothelium, which lines the walls of blood and lymph vessels.

The researchers observed the effect of the synthetically produced molecule, JK - 31, on the growth and proliferation of a model human breast cancer cell line and found that it effectively blocked the protein cyclin - dependent kinase 1 (CDK1), which plays a key part in the process of the division of cancer cells, and therefore inhibited the proliferation of the cells.

To see whether cancer stem cell renewal involves a chain of events similar to that used by embryonic stem cells, and whether the process was affected by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two human breast cancer cell lines that responded to low oxygen by ramping up production of the protein ALKBH5, which removes methyl groups from mRNAs.

«We compared the ability of RSV and parainfluenza virus (PIV3)-- another common virus in children that causes much less severe airway disease — to infect and cause inflammatory responses in a cell culture model of human epithelial cells, which compose the lining of the lung airway.

The final guidelines on research with human embryonic stem cells issued on Monday by the National Institutes of Health set out criteria for determining which ES cell lines can be used in federally funded experiments and give NIH discretion to approve old lines that don't meet stringent modern ethical requirements.

Studies in diabetic rodents suggest that diabetes may alter the intestine's mucosal lining, which is maintained and regenerated by intestinal stem cells; however, little is known about how this occurs and whether it is relevant to humans.

The finding potentially paves the way for scores of labs to generate new stem cell lines without cloned embryos, which had long been considered the only realistic way of making human stem cells in the short run.

They used the gene editing technology CRISPR to engineer a series of human embryonic stem cell lines, which were identical apart from the number of DNA repeats that occurred at the ends of their HTT genes.

After exploiting a technology that allowed them to activate each of nearly 16,000 genes individually in human melanoma cell lines containing mutant BRAF, the authors then treated the panel of cells with the drugs and monitored which cells showed altered drug sensitivity.

Where the second type of condensin, which is present in humans and other multicellular organisms, binds during cell division is another future line of inquiry.

In preclinical experiments using human prostate cancer cell lines, Fu's team showed that increased PLK1 expression activated an oncogene known as c - RAF, which has previously been shown to play a role in regulating cell growth and division.

Obama and Clinton both voted for the Stem Cell Research Enhancement Act, which would have broadly extended federal funding to human embryonic stem cell lines but was vetoed by Bush in 2006 and 2Cell Research Enhancement Act, which would have broadly extended federal funding to human embryonic stem cell lines but was vetoed by Bush in 2006 and 2cell lines but was vetoed by Bush in 2006 and 2007.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channel.

«With our sequencing and transcriptional data, we can figure out which of these cell lines most closely match the human tumors.

Upon excitation, the molecule, which was otherwise not harmful to the cells, killed the cell population of the human HeLa cell line to almost 100 percent.

To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer of human endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.

«We set out to test this in cell lines derived from mice and humans, and came up with a result we definitely did not expect — which then led us to dig deeper for answers.»

Nonetheless, passage of the measure marks a significant milestone in efforts to overturn the Bush policy, which limits government funding to research using 60 human ES cell lines derived before 9 August, 2001.

For while the new NIH guidelines explicitly permit funding for research on stem cell lines in which human embryos have already been destroyed, they also explicitly forbid funding for research on stem cell lines that have been produced by SCNT (see section V. part B).

Indeed, human pluripotent stem cell lines and derivatives, which express a disease - related mutated gene, represent a relevant and pertinent disease cell model to analyse pathological mechanisms through large - scale drug screenings.

He is part of the pioneering groups which established human ES cell lines and distributed them to other scientists free of charge.

To do so, they started with a human embryonic stem cell line, which they chemically nudged to become cells that form what's known as the primitive streak on the hollow ball of cells of the early embryo.

Peter and colleagues focused their initial studies on a standard panel, known as NCI60, of 60 human tumor cell lines that can genetically be divided into two large groups, which they called superclusters 1 and 2.

He added: «Truly naive human ES cell lines would not only help answer fundamental questions about how we are made, and be useful for drug screening and tissue therapy, but they would also provide a benchmark against which other types of stem cells could be measured in terms of their effectiveness in stem cell therapy and regenerative medicine.»

Eliminating these constraints, hepatocytes differentiated from human induced pluripotent stem (hiPS) cells provide an unlimited supply of functional hepatocytes from each parental cell line, which can be derived noninvasively from a diseased or healthy person.

It also works in cells taken directly from human patients, which are usually much more difficult to manipulate than human cell lines grown specifically for lab research.

The team then selected cells from these organoids and placed them inside the Intestine - Chips, which are about the size of AA batteries and re-create the natural microenvironment of the human intestine, including the intestinal epithelium — the layer of cells that forms the lining of both the small and large intestines.

The DNA in our cells, which contains the genetic instructions that make us human, is passed on from parent to child along an unending line of generations.

The use of higher resolution techniques, such as array CGH and, soon, whole genome sequencing, will enhance the ability of researchers to examine stem cell lines to determine which are best — least likely to result in diseases and other problems — to use in creating therapies for use in humans.

We are the home of the UK Stem Cell Bank which provides a world - leading resource of ethically - sourced, quality - controlled human embryonic and other stem cell lines for research applicatiCell Bank which provides a world - leading resource of ethically - sourced, quality - controlled human embryonic and other stem cell lines for research applicaticell lines for research applications.

We developed and implemented such a nomenclature for the European human pluripotent stem cell registry (hPSCreg), which needs to: 1) unambiguously identify a registered cell line; 2) allow tracing of subclones of a particular line; and 3) enable the assignment of different lines to a specific donor origin.

Finally, he opened the door to funding research involving stem cell lines created by producing human embryos by somatic cell nuclear transfer or other means specifically for research in which they are killed.

Each chip, which is approximately the size of an AA battery, features tiny channels lined with tens of thousands of living human cells, recreating the smallest functional unit of an organ.

Now Carette and co-workers have used a similar approach and used a human cell line, in which nearly all human chromosomes are present in a single copy.

A team led by Young - sup Yoon, MD, PhD developed a new method for generating endothelial cells, which make up the lining of blood vessels, from human induced pluripotent stem cells..

Having established the importance of the miR - 181a target genes, Smad1 and Smad5, in BMP / GDF ligand - induced neurite growth, we next determined whether miR - 181a - modulated Smad phosphorylation / activation and neurite growth in SH - SY5Y cells, which are a widely used cell line model of human mDA neurons which also express the miR - 181a targets, BMPR2 and Smad1 / 5 [18,38].

Adenoviral vectors can be produced by human embryonic kidney (HEK293) and human embryonic retinal (PER.C6) cell lines (31), both of which also can be used for lentiviral vector production.

In this study, published in Stem Cell Reports, the researchers used both murine and human pluripotent stem cell lines with airway secretory lineage reporters, which enables their tracking, purification and profilCell Reports, the researchers used both murine and human pluripotent stem cell lines with airway secretory lineage reporters, which enables their tracking, purification and profilcell lines with airway secretory lineage reporters, which enables their tracking, purification and profiling.

The organizers of the project, called GP - write (for work in model organisms and plants) or sometimes HGP - write (for work in human cell lines), envision it as a successor to the Human Genome Project (retroactively termed HGP - read), which 25 years ago promoted rapid advances in DNA sequencing technohuman cell lines), envision it as a successor to the Human Genome Project (retroactively termed HGP - read), which 25 years ago promoted rapid advances in DNA sequencing technoHuman Genome Project (retroactively termed HGP - read), which 25 years ago promoted rapid advances in DNA sequencing technology.

It was discovered the amount of enzymes which damage cartilage is limited when a compound known as diallyl disulphide is introduced to a laboratory human cartilage cell - line.

In another study, 11 human cancer cell lines (carcinomas and glioblastomas) were exposed to ascorbic acid in which 55 % of the cell lines were more susceptible (EC50 ≤ 20 mmol / L) and 45 % were more resistant (EC50 > 20 mmol / L) to incubation.

Year 6 Science Assessments and Tracking Objectives covered: Describe how living things are classified into broad groups according to common observable characteristics and based on similarities and differences, including micro-organisms, plants and animals Give reasons for classifying plants and animals based on specific characteristics Identify and name the main parts of the human circulatory system, and describe the functions of the heart, blood vessels and blood Recognise the impact of diet, exercise, drugs and lifestyle on the way their bodies function Describe the ways in which nutrients and water are transported within animals, including humans Recognise that living things have changed over time and that fossils provide information about living things that inhabited the Earth millions of years ago Recognise that living things produce offspring of the same kind, but normally offspring vary and are not identical to their parents Identify how animals and plants are adapted to suit their environment in different ways and that adaptation may lead to evolution Recognise that light appears to travel in straight lines Use the idea that light travels in straight lines to explain that objects are seen because they give out or reflect light into the eye Explain that we see things because light travels from light sources to our eyes or from light sources to objects and then to our eyes Use the idea that light travels in straight lines to explain why shadows have the same shape as the objects that cast them Associate the brightness of a lamp or the volume of a buzzer with the number and voltage of cells used in the circuit Compare and give reasons for variations in how components function, including the brightness of bulbs, the loudness of buzzers and the on / off position of switches Use recognised symbols when representing a simple circuit in a diagram