Sentences with phrase «human cell lines with»

The work, funded by the US National Human Genome Research Institute, aims to create human cell lines with subtly different genomes in order to test ideas about which mutations cause disease and how.

Professor Ali Tavassoli, who led the study with colleague Dr. Ishna Mistry, explains: «In an effort to better understand the role of HIF - 1 in cancer, and to demonstrate the potential for inhibiting this protein in cancer therapy, we engineered a human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environment.

Thus, high ALKBH1 activity can globally decrease the amount of proteins made in a cell, as the researchers observed in a human cell line with abnormally high levels of ALKBH1.

Overall, making a human cell line with a gene knockout was straightforward, and is a testament to how far genome engineering has come.

Not exact matches

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

Cholesterol is carried in the blood and is infused into the cells lining the blood vessels, says Prof. Seneviratne dealing with the chemistry of the human body.

Before we established in - house human taste cell (HTC) technologies, it was not possible to use human tongue - derived cells as a model, due to the lack of homogenous, proliferating cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.

To create the effect of tobacco smoke on cells, Vaz, Baylin and their colleagues began their studies with human bronchial cells, which line the airways of the lungs, and grew them in a laboratory.

Introducing new drugs to plastic microchips lined with human cells should let researchers watch what happens without ever involving a living creature.

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«This model was trained on genetic data from human tumors in The Cancer Genome Atlas and was able to predict response to certain inhibitors that affect cancers with overactive Ras signaling in an encyclopedia of cancer cell lines,» Greene said.

Although the researchers emphasized that laboratory results involving cell lines and mice do not necessarily translate to human treatment, they say their findings show that new mTOR inhibitors combined with chemotherapy could become a new treatment strategy for T - ALL.

Human placental cell line Jeg - 3 can be readily infected by a circulating strain of Zika virus (left panel) and completely protected by treatment with nanchangmycin (right panel).

Using both fruit fly and human lung cancer cell lines, researchers targeted two of the most common genetic mutations associated with NSCLC — Ras and PTEN (P13K).

Cell lines from human tumours did exist, but were considered unsafe: what if cancerous cells were transferred along with the vaccine?

In May 2005, Hwang and his colleagues reported that it had produced 11 new human embryonic stem (ES) cell lines that carried the genetic signature of patients with diabetes, spinal cord injury, or a genetic blood disorder (Science, 20 May, p. 1096).

Tests with kidney cancer and two human uterine sarcoma cell lines, one with multidrug resistance, showed that 10 of these new compounds were impressively potent against all three cell lines, the researchers reported.

While he has been working with federally - approved human ES lines, he notes that those lines could be considered illegal until they get vetted by the stem cell working group NIH plans to create.

The final guidelines on research with human embryonic stem cells issued on Monday by the National Institutes of Health set out criteria for determining which ES cell lines can be used in federally funded experiments and give NIH discretion to approve old lines that don't meet stringent modern ethical requirements.

The bill was put forth to loosen the restrictions Bush placed on human embryonic stem cell research on August 9, 2001, when he banned federal funding for work with any stem cell line created after that date.

In the chamber, tubes about the thickness of a human hair were lined with endothelial cells as in natural blood vessels.

After exploiting a technology that allowed them to activate each of nearly 16,000 genes individually in human melanoma cell lines containing mutant BRAF, the authors then treated the panel of cells with the drugs and monitored which cells showed altered drug sensitivity.

During the last several years, research efforts with human cell lines revealed that the genomic loci encoding tRNAs give rise to shorter tRNA fragments («tRFs»).

In a statement put on the Web this morning, NIH reported that all 64 lines «show characteristics of stem cell morphology» and have undergone several population doublings, and most of them have demonstrated all the protein markers «known to be associated with human embryonic stem cells.»

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

Earlier this year, researchers in Germany published a scientific paper that described the first sequence of the full HeLa genome, comparing the DNA of HeLa cell lines with that of cells from healthy human tissues.

«With our sequencing and transcriptional data, we can figure out which of these cell lines most closely match the human tumors.

In a paper published online May 17, 2016 in the journal Nature Communications, de Jong and co-first author Koji Taniguchi, MD, PhD, and colleagues, report that treating both ERK1 / 2 and the compensatory pathway, ERK5, concurrently with a combination of drug inhibitors halted colorectal cancer growth more effectively in both mouse models and human colorectal cancer cell lines.

His team have begun recolonising the primate scaffolds with human cells that line blood vessels, the first step towards human - scale biolimb development, and have started experiments using human myoblasts in rats instead of the mice ones.

To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer of human endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.

«We set out to test this in cell lines derived from mice and humans, and came up with a result we definitely did not expect — which then led us to dig deeper for answers.»

Dr. Bauman and her colleagues treated human head and neck cancer cells in the laboratory with varying doses of sulforaphane and a control, and compared them to normal, healthy cells that line the throat and mouth.

They tried hundreds of different recipes; eventually they discovered that if they mixed liver precursor cells (derived from iPS cells) with two other types of standard human cell lines known to be important for embryonic liver development, then the cells would spontaneously form a 4 to 5 - millimeter 3D structure called a liver bud.

«If there are 60 cell lines, that's news to me — and good news,» says cell biologist Douglas Melton of Harvard University, who has been working with both animal and human ES cells.

Attempting to find middle ground in the contentious debate over human embryonic stem cells, President George W. Bush announced 9 August that the National Institutes of Health (NIH) will be allowed to fund work with embryonic stem (ES) cell lines — but only those lines that have already been derived.

The human airway is lined with two kinds of epithelial cells — those that possess hairlike fringes called cilia and those that don't.

Despite the ability of R3A to utilize both CCR5 and CXCR4 to infect cell lines, in human CD4 + T cells stimulated with anti-CD3 / anti-CD28 coated magnetic beads, CCR5 is downregulated causing transient resistance to R5 HIV [47].

Recent studies with human breast cancer cell lines have also shown that Shp2 mediates survival signals in cancer cells.

For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.

Pharmacology of human dopamine D3 receptor expressed in a mammalian cell line: Comparison with D2 receptor

Establishment of a human embryonic germ cell line and comparison with mouse and human embryonic stem cells.

Until now, scientists examining the causes and effects of insulin resistance have struggled with a general lack of human cell lines from tissues such as muscle, fat and liver that respond significantly to insulin, Kahn says.

To do so, they started with a human embryonic stem cell line, which they chemically nudged to become cells that form what's known as the primitive streak on the hollow ball of cells of the early embryo.

TNA3: BPRC will offer access to its library of human cell reporter assays for the identification of innate immune receptor - induced signalling cascades, including human cell lines transfected with PAMPs such as TLR and / or engineered to express luciferase in response to NF - kB or IFRE - mediated signal transduction, and cell lines that provide insight into the intracellular signalling cascades of innate immune receptors.

In cultured human cells and in rabbits with implanted stents, Finn and colleagues showed that metformin augmented the effect of mTOR inhibitors on regrowth of the blood vessel lining.

IHC - P mouse tumor tissue (from lung) with human cell line injected, some muscle tissue attached as well sees high background for human cellswith priamry Ab as well as isotype ctrl, but also for muscle (does not contain any EGF) Ab: 1 ug /...

NeuroStemcell is focused on the identification and systematic comparison of progenitor cell lines with the most favourable characteristics for mesDA and striatal GABAergic neuronal differentiation, generated either directly from human embryonic stem (ES) cells, from Neural Stem (NS) cells derived from ES cells or fetal brain, from induced Pluripotent Stem (iPS) cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate cells in appropriate animals models.

The findings from this one cell line are also inconsistent with clinical data from preimplantation genetic diagnosis, further suggesting that the stem cell assay may not wholly reflect what happens during human development.

Human cord - blood T - cells infected with co-cultivation with HTLV - positive T - cell lines.

HAT sensitive derivative of CEM, a human T - cell line derived from the peripheral blood buffy coat of a four - year old Caucasian female with acute lymphoblastic leaukemia.