In addition, knockdown of STAT6 by short hairpin RNAs results in apoptosis of
human colon cancer HT - 29 cells (Zhang et al., 2005).
For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h.
Human colon cancer cell line
HT - 29 and prostate
cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.