Not exact matches
Some of the researchers at the centre will study the
differentiation of
stem cells into other
cell types, one group by using
human embryonic stem cell biology and another by studying early embryo development.
The laboratory process, described in the journal Scientific Reports, entails genetically modifying a line of
human embryonic stem cells to become fluorescent upon their
differentiation to retinal ganglion
cells, and then using that
cell line for development of new
differentiation methods and characterization of the resulting
cells.
Human embryonic stem cells can exist in two different states that are termed naïve (the ground state) and primed (the state before
differentiation into a specialised
cell).
Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing
human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural
differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.
«We saw better germ -
cell differentiation in this transplantation model than we've ever seen,» said Renee Reijo Pera, PhD, former director of Stanford's Center for Human Embryonic Stem Cell Research and Educat
cell differentiation in this transplantation model than we've ever seen,» said Renee Reijo Pera, PhD, former director of Stanford's Center for
Human Embryonic Stem Cell Research and Educat
Cell Research and Education.
NeuroStemcell is focused on the identification and systematic comparison of progenitor
cell lines with the most favourable characteristics for mesDA and striatal GABAergic neuronal
differentiation, generated either directly from
human embryonic stem (ES) cells, from Neural Stem (NS) cells derived from ES cells or fetal brain, from induced Pluripotent Stem (iPS) cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate cells in appropriate animals mod
stem (ES)
cells, from Neural
Stem (NS) cells derived from ES cells or fetal brain, from induced Pluripotent Stem (iPS) cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate cells in appropriate animals mod
Stem (NS)
cells derived from ES
cells or fetal brain, from induced Pluripotent
Stem (iPS) cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate cells in appropriate animals mod
Stem (iPS)
cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate
cells in appropriate animals models.
Isolation and directed
differentiation of neural crest
stem cells derived from
human embryonic stem cells.
Synthetic peptide - acrylate surfaces for long - term self - renewal and cardiomyocyte
differentiation of
human embryonic stem cells.
Differentiation of
human pluripotent
stem cells (
embryonic stem cells (hESCs) and induced pluripotent
stem cells (hiPSCs)-RRB- may be a useful strategy to create the supply to meet the high demand, although this currently remains inefficient or includes the use of xenogeneic and undefined reagents.
Calder A, Roth - Albin I, Bhatia S, et al., Lengthened G1 phase indicates
differentiation status in
human embryonic stem cells.
By: Sadhana Agarwal, Katherine L. Holton, Robert Lanza
Differentiation of
human embryonic stem cells (hESCs) to specific functional
cell types can be achieved using methods that mimic in vivo
embryonic developmental programs.
Forced aggregation of defined numbers of
human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic
differentiation.
Non-cardiomyocytes influence the electrophysiological maturation of
human embryonic stem cell - derived cardiomyocytes during
differentiation.
Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), are known to be vulnerable to apoptosis upon various technical manipulation, such as single cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer, differentiation and FACS cell sor
Human pluripotent
stem cells (hPSCs), including
human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), are known to be vulnerable to apoptosis upon various technical manipulation, such as single cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer, differentiation and FACS cell sor
human embryonic stem cells (hESCs) and
human induced pluripotent stem cells (hiPSCs), are known to be vulnerable to apoptosis upon various technical manipulation, such as single cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer, differentiation and FACS cell sor
human induced pluripotent
stem cells (hiPSCs), are known to be vulnerable to apoptosis upon various technical manipulation, such as single
cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer,
differentiation and FACS
cell sorting.
Both mouse and
human iPSCs are similar to
embryonic stem cells (ESCs) with respect to their morphology,
cell behavior, gene expression, epigenetic status and
differentiation potential both in culture and in vivo.
Dynamic chromatin remodeling mediated by polycomb proteins orchestrates pancreatic
differentiation of
human embryonic stem cells.
Susan Amara, USA - «Regulation of transporter function and trafficking by amphetamines, Structure - function relationships in excitatory amino acid transporters (EAATs), Modulation of dopamine transporters (DAT) by GPCRs, Genetics and functional analyses of
human trace amine receptors» Tom I. Bonner, USA (Past Core Member)- Genomics, G protein coupled receptors Michel Bouvier, Canada - Molecular Pharmacology of G protein - Coupled Receptors; Molecular mechanisms controlling the selectivity and efficacy of GPCR signalling Thomas Burris, USA - Nuclear Receptor Pharmacology and Drug Discovery William A. Catterall, USA (Past Core Member)- The Molecular Basis of Electrical Excitability Steven Charlton, UK - Molecular Pharmacology and Drug Discovery Moses Chao, USA - Mechanisms of Neurotophin Receptor Signaling Mark Coles, UK - Cellular
differentiation,
human embryonic stem cells, stromal
cells, haematopoietic
stem cells, organogenesis, lymphoid microenvironments, develomental immunology Steven L. Colletti, USA Graham L Collingridge, UK Philippe Delerive, France - Metabolic Research (diabetes, obesity, non-alcoholic fatty liver, cardio - vascular diseases, nuclear hormone receptor, GPCRs, kinases) Sir Colin T. Dollery, UK (Founder and Past Core Member) Richard M. Eglen, UK Stephen M. Foord, UK David Gloriam, Denmark - GPCRs, databases, computational drug design, orphan recetpors Gillian Gray, UK Debbie Hay, New Zealand - G protein - coupled receptors, peptide receptors, CGRP, Amylin, Adrenomedullin, Migraine, Diabetes / obesity Allyn C. Howlett, USA Franz Hofmann, Germany - Voltage dependent calcium channels and the positive inotropic effect of beta adrenergic stimulation; cardiovascular function of cGMP protein kinase Yu Huang, Hong Kong - Endothelial and Metabolic Dysfunction, and Novel Biomarkers in Diabetes, Hypertension, Dyslipidemia and Estrogen Deficiency, Endothelium - derived Contracting Factors in the Regulation of Vascular Tone, Adipose Tissue Regulation of Vascular Function in Obesity, Diabetes and Hypertension, Pharmacological Characterization of New Anti-diabetic and Anti-hypertensive Drugs, Hypotensive and antioxidant Actions of Biologically Active Components of Traditional Chinese Herbs and Natural Plants including Polypehnols and Ginsenosides Adriaan P. IJzerman, The Netherlands - G protein - coupled receptors; allosteric modulation; binding kinetics Michael F Jarvis, USA - Purines and Purinergic Receptors and Voltage-gated ion channel (sodium and calcium) pharmacology Pain mechanisms Research Reproducibility Bong - Kiun Kaang, Korea - G protein - coupled receptors; Glutamate receptors; Neuropsychiatric disorders Eamonn Kelly, Prof, UK - Molecular Pharmacology of G protein - coupled receptors, in particular opioid receptors, regulation of GPCRs by kinasis and arrestins Terry Kenakin, USA - Drug receptor pharmacodynamics, receptor theory Janos Kiss, Hungary - Neurodegenerative disorders, Alzheimer's disease Stefan Knapp, Germany - Rational design of highly selective inhibitors (so call chemical probes) targeting protein kinases as well as protein interaction inhibitors of the bromodomain family Andrew Knight, UK Chris Langmead, Australia - Drug discovery, GPCRs, neuroscience and analytical pharmacology Vincent Laudet, France (Past Core Member)- Evolution of the Nuclear Receptor / Ligand couple Margaret R. MacLean, UK - Serotonin, endothelin, estrogen, microRNAs and pulmonary hyperten Neil Marrion, UK - Calcium - activated potassium channels, neuronal excitability Fiona Marshall, UK - GPCR molecular pharmacology, structure and drug discovery Alistair Mathie, UK - Ion channel structure, function and regulation, pain and the nervous system Ian McGrath, UK - Adrenoceptors; autonomic transmission; vascular pharmacology Graeme Milligan, UK - Structure, function and regulation of G protein - coupled receptors Richard Neubig, USA (Past Core Member)- G protein signaling; academic drug discovery Stefan Offermanns, Germany - G protein - coupled receptors, vascular / metabolic signaling Richard Olsen, USA - Structure and function of GABA - A receptors; mode of action of GABAergic drugs including general anesthetics and ethanol Jean - Philippe Pin, France (Past Core Member)- GPCR - mGLuR - GABAB - structure function relationship - pharmacology - biophysics Helgi Schiöth, Sweden David Searls, USA - Bioinformatics Graeme Semple, USA - GPCR Medicinal Chemistry Patrick M. Sexton, Australia - G protein - coupled receptors Roland Staal, USA - Microglia and neuroinflammation in neuropathic pain and neurological disorders Bart Staels, France - Nuclear receptor signaling in metabolic and cardiovascular diseases Katerina Tiligada, Greece - Immunopharmacology, histamine, histamine receptors, hypersensitivity, drug allergy, inflammation Georg Terstappen, Germany - Drug discovery for neurodegenerative diseases with a focus on AD Mary Vore, USA - Activity and regulation of expression and function of the ATP - binding cassette (ABC) transporters
Accelerated Three - Dimensional Neuroepithelium Formation from
Human Embryonic Stem Cells and Its Use for Quantitative
Differentiation to
Human Retinal Pigment Epithelium.
Currently,
stem cell research focuses on renewal and
differentiation of
stem cells and the molecular mechanisms of its pluripotency - or their ability to develop into any type of
cell - using
human embryonic stem cells, induced pluripotent
stem cells, and
stem cells in simpler organisms.