Sentences with phrase «human fibroblast cells»

Proline is needed to build collagen, to increase collagen synthesis in human fibroblast cells.

They have already shown that they can transform human fibroblast cells into pluripotent stem cells, and now plan to start working on delivering the proteins needed to differentiate stem cells into specialized tissues.

Hamlyn, working with Richard Schmidt of the School of Pharmacy at the University of Wales in Cardiff, exposed human fibroblast cells to six fungi strains.

The «target» cells on the other side of the BeWo barrier to the nanoparticles were human fibroblast cells, found in skin and connective tissue.

Not exact matches

Working with human breast tissue, the new study's authors attempted to induce EMT in normal cells; they figured they would just get fibroblasts, a type of connective tissue that is important in wound healing.

The team also tested nanoparticle absorption for other kinds of human cells, including fibroblasts from donors of older ages and found significant differences.

Avivi's team has found out that fibroblast skin cells from the armpits of the rats can kill human cancer cells in a dish.

In studies with human fibroblasts that make up connective tissue, Boger's team tested whether NOD1 activity could affect CMV replication in cultures of cells grown in the lab.

Departing from prior studies, the team collected epithelial cells and fibroblasts from human sources: one cadaver and four living people who had their healthy voice boxes removed during unrelated surgeries.

«We also showed for the first time that AHR signaling in primary human orbital fibroblasts disrupts the pro-fibrotic Wnt / β - catenin pathway, which is important for scar cell formation,» noted Dr. Phipps.

Now Yamanaka and his colleagues report in the journal Cell that the same combination of genes induced pluripotency in commercially available human fibroblasts (connective tissue cells that play a crucial role in healing) derived from the facial skin of a 36 - year - old woman, the joint tissue of a man, aged 69, and a newborn, respectively.

And when he checked, Avivi found that the fibroblasts and their secretions didn't harm healthy human cells.

As controls, fibroblasts and secretions from normal lab rats, mice, and another rodent called the spiny mouse were powerless to stop the human cancer cells growing.

The fibroblasts rapidly killed the human cancer cells.

In vitro experiments used human colon carcinoma cells with and without MMP9 and mouse embryonic fibroblasts, which are connective tissue cells that make the extracellular matrix and collagen and play an important role in tissue repair.

Researchers used the CRISPR / Cas9 gene editing technique to introduce a segment of a human gene causing Huntington's, with a very long glutamine repeat region, into pig fibroblast cells.

The study carried out at the University of Eastern Finland used the induced pluripotent stem cell technology, which enables the generation of pluripotent stem cells from human skin fibroblasts.

In a study published recently in the journal Carbon, the team grew human and mouse fibroblast cells (cells involved in wound healing) on flat graphene sheets and on wrinkled ones.

For the new study, the team used a cell - reprogramming technique (similar to those used to reprogram skin cells into stem cells) to generate human DRG - type sensory neurons from ordinary skin cells called fibroblasts.

The researchers were also able to control the relative composition of different hormonal cell types simply by exposing human pluripotent stem cells to different ratios of two proteins: fibroblast growth factor 8 and bone morphogenetic protein 2.

«The resulting hydrogel composed of physically self - assembled CarHC polymers exhibited a rapid gel - sol transition on light exposure, which enabled the facile release / recovery of 3T3 fibroblasts and human mesenchymal stem cells (hMSCs) from 3D cultures while maintaining their viability.»

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

↵ 3 The abbreviations used are: HUVEC, human umbilical vein endothelial cell; DiI - Ac - LDL, 1,1 ′ - dioctadecyl - 3,3,3 ′, 3 ′ - tetramethyl - indocarbocyanine acetylated low - density lipoprotein; FACS, fluorescence - activated cell sorting; FGFR, fibroblast growth factor receptor; Flk, fetal liver kinase; ICAM, intercellular adhesion molecule; mAb, monoclonal antibody; PE, phycoerythrin; TNF, tumor necrosis factor; VCAM, vascular cell adhesion molecule.

Rho - kinase inhibitor Y - 27632 increases cellular proliferation and migration in human foreskin fibroblast cells

Xu and his team, which includes researchers from Penn's departments of Dermatology and Biology, as well as the New Jersey Institute of Technology, started with human skin cells called dermal fibroblasts.

Human embryonic stem cells derived from affected embryos during a pre-implantation diagnostic (PGD), as well as the conversion of somatic cells, such as skin fibroblasts, into induced pluripotent stem cells by genetic manipulation, offer the unique opportunity to have access to a large spectrum of disease - specific cell models.

It has recently been demonstrated that mouse and human fibroblasts can be reprogrammed into an embryonic stem cell - like state by introducing combinations of four transcription factors.

Immortalized Fibroblast - Like Cells Derived from Human Embryonic Stem Cells Support Undifferentiated Cell Growth.

Induction of stem cell gene expression in adult human fibroblasts without transgenes.

Autocrine fibroblast growth factor 2 increases the multipotentiality of human adipose - derived mesenchymal stem cells.

Lung fibroblasts accelerate wound closure in human alveolar epithelial cells through hepatocyte growth factor / c - Met signaling.

Yamanaka's group used human adult dermal fibroblasts and induced them to become iPSCs, appearing and functioning like hESCs, by having them express the same proteins as he used with mouse cells: Oct - 4, Sox2, Klf4, and c - Myc (Takahashi et al., 2007).

His research is focused on retinal regeneration by reprogramming human fibroblasts either into induced pluripotent stem cells or directly into photoreceptors.

SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells.

Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts.

HuH - 7 (human HCC), HepG2 (human hepatocellular blastoma), 293T (human embryonic kidney fibroblast), HeLa (human cervical cancer cells), NIH 3T3 (murine fibroblast), and HCT116 (human colon cancer) and its derived cell line HCT116 p21 − / − (kindly provided by Dr. B. Vogelstein, Howard Hughes Medical Institute, Chevy Chase and the Sydney Kimmel Comprehensive Cancer Center of the Johns Hopkins University School of Medicine, Baltimore, MD; ref.

Oct4, Sox2, c - Myc, and Klf4 were the four original factors that were capable of converting mouse and human fibroblasts into iPs cells [1], [3], [5], [11], [12].

Citation: Li C, Yu H, Ma Y, Shi G, Jiang J, Gu J, et al. (2009) Germline - Competent Mouse - Induced Pluripotent Stem Cell Lines Generated on Human Fibroblasts without Exogenous Leukemia Inhibitory Factor.

Normal human lung fibroblasts (NHLF) cells grown in 384 - well Optilux plates were treated with candidate compounds and incubated for 18 hours prior to fixation and staining with Hoescht dye and anti-HMOX1 antibody as described in Materials and Methods.

With current methodologies, mouse embryonic fibroblast (MEF) cells have been used as a feeder layer to derive both mouse and human iPS cells.

In this study, we develop a reproducible protocol for efficient reprogramming mouse neural progenitor cells (NPCs) on human foreskin fibroblast (HFF) cells via retroviral transfer of human transcriptional factors OCT4 / SOX2 / KLF4 / C - MYC.

While scientists have successfully reprogrammed different types of mouse cells (fibroblasts, liver and intestinal cells), skin fibroblasts were the only human cell type they had ever tried their hands on.

The hESC colonies were mechanically divided and seeded as small aggregates onto 12 - well plates (CellBIND Surface, Corning, Inc., Corning, NY), containing gamma - irradiated human foreskin fibroblasts (CRL - 2429, ATCC, Manassas, VA) as feeder cells.

The researchers then successfully prodded what they call keratinocyte - derived iPS cells or KiPS cells to distinguish them from fibroblast - derived iPS cells into becoming all the cell types in the human body, including heart muscle cells and dopamine - producing neurons, which are affected by Parkinson's disease.

While this manuscript was in preparation, a similar episomal strategy was used to reprogram human primary fibroblasts using a distinct pluripotent cocktail of factors, validating our methods, but with significant lower efficiency (only 3 to 6 colonies / 106 input cells)[42].

Pluripotent stem cells can be generated from adult mouse - tail tip fibroblasts and adult human fibroblasts by the retrovirus - mediated transfection of four transcription factors, Oct3 / 4, Sox2, c - Myc, and Klf4.

The adult human heart is only half muscle; the other half is made of support cells called fibroblasts that help repair wounds and form scars around damaged tissue.

Using human colon cancer cells and primary human fibroblasts isolated from tumors and adjacent normal tissues, Alexandros Glentis and colleagues at the Institut Curie addressed the question of whether the cancer cells or the CAF cells were responsible for the breakdown of the basement membrane that leads to cancer progression.

W. Hu et al., «Direct conversion of normal and Alzheimer's disease human fibroblasts into neuronal cells by small molecules,» Cell Stem Cell, doi: 10.1016 / j.stem.2015.07.006, 2015.

Citation: Byrne JA, Nguyen HN, Reijo Pera RA (2009) Enhanced Generation of Induced Pluripotent Stem Cells from a Subpopulation of Human Fibroblasts.