Sentences with phrase «human fibroblasts by»

Pluripotent stem cells can be generated from adult mouse - tail tip fibroblasts and adult human fibroblasts by the retrovirus - mediated transfection of four transcription factors, Oct3 / 4, Sox2, c - Myc, and Klf4.

Not exact matches

The researchers were also able to control the relative composition of different hormonal cell types simply by exposing human pluripotent stem cells to different ratios of two proteins: fibroblast growth factor 8 and bone morphogenetic protein 2.

Human embryonic stem cells derived from affected embryos during a pre-implantation diagnostic (PGD), as well as the conversion of somatic cells, such as skin fibroblasts, into induced pluripotent stem cells by genetic manipulation, offer the unique opportunity to have access to a large spectrum of disease - specific cell models.

Despite overexpression of mRNA from the XpdTTD allele relative to the wt allele (Figure 1E), TFIIH protein levels were reduced by 50 % in primary mouse XpdTTD / TTD fibroblasts (Figure 4E and 4F), thereby mimicking the situation in human patients with TTD.

It has recently been demonstrated that mouse and human fibroblasts can be reprogrammed into an embryonic stem cell - like state by introducing combinations of four transcription factors.

Yamanaka's group used human adult dermal fibroblasts and induced them to become iPSCs, appearing and functioning like hESCs, by having them express the same proteins as he used with mouse cells: Oct - 4, Sox2, Klf4, and c - Myc (Takahashi et al., 2007).

His research is focused on retinal regeneration by reprogramming human fibroblasts either into induced pluripotent stem cells or directly into photoreceptors.

HuH - 7 (human HCC), HepG2 (human hepatocellular blastoma), 293T (human embryonic kidney fibroblast), HeLa (human cervical cancer cells), NIH 3T3 (murine fibroblast), and HCT116 (human colon cancer) and its derived cell line HCT116 p21 − / − (kindly provided by Dr. B. Vogelstein, Howard Hughes Medical Institute, Chevy Chase and the Sydney Kimmel Comprehensive Cancer Center of the Johns Hopkins University School of Medicine, Baltimore, MD; ref.

The researchers then successfully prodded what they call keratinocyte - derived iPS cells or KiPS cells to distinguish them from fibroblast - derived iPS cells into becoming all the cell types in the human body, including heart muscle cells and dopamine - producing neurons, which are affected by Parkinson's disease.

Formic acid is a product of the α - oxidation of fatty acids by human skin fibroblasts: deficiency of formic acid production in peroxisome - deficient fibroblasts

W. Hu et al., «Direct conversion of normal and Alzheimer's disease human fibroblasts into neuronal cells by small molecules,» Cell Stem Cell, doi: 10.1016 / j.stem.2015.07.006, 2015.

In their study, published alongside Deng's in Cell Stem Cell, the researchers converted human fibroblasts to neurons using a combination of seven molecules applied to the cells for one week, followed by a three - week maturation period in a medium containing two of the reprogramming molecules and one other molecule.

The research team began by injecting all candidate genes into the human fibroblasts.