The explant model was incubated in
Human foetal RPE medium at 37 °C in 5 % CO2 for 3 or 12 hours.
Cells were cultured in
human foetal RPE medium [30] where, within 13 days, they formed a pigmented monolayer with characteristic RPE cobblestone appearance similar to that observed in human post-mortem RPE sheets and HESC - derived RPE cells [9](Fig. 1B).
Labelled outer segments were washed, resuspended in
human foetal RPE medium and seeded onto iPS - RPE cells cultured on gelatin - coated 35 mm dishes.
Not exact matches
The difficulties associated with obtaining nerve tissue at the correct stage of development and differentiation from aborted embryos means that
foetal tissue transplantation is no longer in favour, but the creation of
human embryos specifically as sources of stem cells, and the push to use «spare» embryos from IVF treatments is gatheringmomentum.
Pro-choice euphemisms like «pre-embryo,» «
foetal tissue,» and «ball of cells» are materialistic and reflect fully the diminishment of
human life as chance — easy come, easy go.
Various cell types have been examined for use in RPE cell replacement including immortalized cell lines, such as the
human RPE cell line, ARPE19 [2], sheets of adult RPE [3],
foetal RPE [4], RPE derived from
human embryonic stem cells (HESC - RPE)[5]--[10] and many non-RPE cells lines [11]--[15].
Exposure to low levels of Bisphenol A during
foetal development has also been shown to lead to a variety of reproductive problems in
humans, including a lowered sperm count and infertile sperm.