An initial library of 15 biodegradable particle formulations was tested for their ability to carry siRNAs into
human glioblastoma cells that were genetically engineered to make green fluorescent protein (GFP).
Human glioblastoma cell line.
Not exact matches
In
human cells and in mice, the virus infected and killed the stem
cells that become a
glioblastoma, an aggressive brain tumor, but left healthy brain
cells alone.
Using
human - derived
glioblastoma cells in a mouse models, researchers found that the modified high - fat, low - carbohydrate diet increased life expectancy by 50 percent while also reducing tumor progression by a similar amount.
The investigators report that trapping virus - loaded stem
cells in a gel and applying them to tumors significantly improved survival in mice with
glioblastoma multiforme, the most common brain tumor in
human adults and also the most difficult to treat.
The team found that exposing samples of
human glioblastoma tumours grown in a dish to the Zika virus destroyed the cancer stem
cells.
Shah and his team loaded the herpes virus into
human MSCs and injected the
cells into
glioblastoma tumors developed in mice.
In a series of experiments, the researchers first identified a set of 19 transcription factors that were expressed at significantly greater levels in cultured
human glioblastoma stem
cells capable of tumor propagation than in differentiated tumor
cells.
Several studies have supported a role for cancer stem
cells in the aggressive brain tumors called
glioblastoma, but those studies involved inducing
human tumors to grow in mice, and as such their relevance to cancer in
humans has been questioned.
Glioblastomas in lab dishes and mouse brains are fakes, little Potemkin villages that everyone thought were faithful replicas of human glioblastomas but which, lacking tumor stem cells, were nothing
Glioblastomas in lab dishes and mouse brains are fakes, little Potemkin villages that everyone thought were faithful replicas of
human glioblastomas but which, lacking tumor stem cells, were nothing
glioblastomas but which, lacking tumor stem
cells, were nothing of the kind.
Another is that the transplanted bits of tumor act nothing like cancers in actual
human brains, Fine and colleagues reported in 2006: Real - life
glioblastomas grow and spread and resist treatment because they contain what are called tumor stem
cells, but tumor stem
cells don't grow well in the lab, so they don't get transplanted into those mouse brains.
Shah next plans to rationally combine the toxin - secreting stem
cells with a number of different therapeutic stem
cells developed by his team to further enhance their positive results in mouse models of
glioblastoma, the most common brain tumor in
human adults.
Into the cerebral cortex of mice with these light - sensitive proteins, the team implanted cancer
cells from a
human pediatric cortical
glioblastoma.
VIRUS VICTORY Zika virus (green) infects and kills stem
cells (red) in
human glioblastoma tissue, without infecting healthy brain
cells.
It is a continuation of previous research, published in 2011, that focused on the effect of decitabine on
glioblastoma human cell cultures.
Figure 2: Abnormal accumulation of the FGFR - TACC fusion protein (red) in
glioblastoma stem
cells isolated from a primary
human glioblastoma with fused FGFR - TACC genes.
Son et al., SSEA - 1 is an enrichment marker for tumor - initiating
cells in
human glioblastoma.
In another study, 11
human cancer
cell lines (carcinomas and
glioblastomas) were exposed to ascorbic acid in which 55 % of the
cell lines were more susceptible (EC50 ≤ 20 mmol / L) and 45 % were more resistant (EC50 > 20 mmol / L) to incubation.