To read and download the publication, see Enhanced Utilization of Induced Pluripotent Stem Cell — Derived
Human Intestinal Organoids Using Microengineered Chips
«We have shown that the hydrogel matrix helps
the human intestinal organoids (HIOs) engraft into the intestinal tissue, that they differentiate and accelerate the healing of the wound,» said Andrés J. García, Regents» Professor in Georgia Tech's Woodruff School of Mechanical Engineering.
«This work provides a proof of principle for using stem cell - derived
human intestinal organoids in a therapeutic setting.»
Not exact matches
To better understand the intestine in its normal and pathological states, researchers have created «
organoids» by isolating
intestinal stem cells from
human biopsy samples.
«Because the primary Small Intestine Chip recapitulates the physical microenvironment that cells experience inside the
human body, such as fluid flow and cyclic peristalsis - like stretching motions, it exhibits a genome - wide gene expression profile that comes closer to its in vivo counterpart than that of the same
intestinal cells grown as 3D
organoids,» said first - author Magdalena Kasendra, Ph.D., a former Postdoctoral Fellow on Ingber's team and now Principal Scientist at Emulate, Inc. in Boston.
The team then selected cells from these
organoids and placed them inside the Intestine - Chips, which are about the size of AA batteries and re-create the natural microenvironment of the
human intestine, including the
intestinal epithelium — the layer of cells that forms the lining of both the small and large intestines.
ANN ARBOR, Mich — By combining engineered polymeric materials known as hydrogels with complex
intestinal tissue known as
organoids — made from
human pluripotent stem cells — researchers have taken an important step toward creating a new technology for controlling the growth of these
organoids and using them for treating wounds in the gut that can be caused by disorders such as inflammatory bowel disease (IBD).
The primary epithelial cells are expanded as 3D
organoids, dissociated, and cultured on a porous membrane within a microfluidic device with
human intestinal microvascular endothelium cultured in a parallel microchannel under flow and cyclic deformation.
Objective:
Human intestinal epithelial
organoids (IEOs) are increasingly being recognised as a highly promising translational research tool.