Sentences with phrase «human kinases»

There are over 500 known human kinases and 10s of thousands of possible proteins they can target.

No. 5,817,479, entitled Human Kinase Homologs, claims 44 ESTs which are fragments of genes encoding protein kinases.

Not exact matches

Cunningham and her postdoc at the time, Christine Kirvan, found that the antibodies literally bind to human neurons, activating an enzyme called calcium / calmodulin - dependent protein (CaM) kinase II.

The researchers observed the effect of the synthetically produced molecule, JK - 31, on the growth and proliferation of a model human breast cancer cell line and found that it effectively blocked the protein cyclin - dependent kinase 1 (CDK1), which plays a key part in the process of the division of cancer cells, and therefore inhibited the proliferation of the cells.

The results showed that, when applied, a long - term inhibition of Rho - kinase signaling increased the expressions of chondrocyte - specific genes and differentiation markers in human chondrosarcoma 2/8 cells.

«There are more than 500 kinases in the human body; and many of them are essential building - blocks of life,» Laufer explains.

The Structural Genomics Consortium at the University of North Carolina at Chapel Hill (SGC - UNC), in partnership with the DiscoverX Corporation, has reached the milestone halfway point in its development of the Kinase Chemogenomic Set, a potent group of inhibitors which allow deeper exploration of the human kinome, a family of enzymes critical to understanding human disease and developing new therapies.

There are an estimated 400 - 500 kinases in the human genome, with literally dozens being activated during disease processes.

This collaborative project between industrial and academic scientists will continue to expand the KCGS with the goal of fully covering all human protein kinases.

There are about 500 kinases in the human genome, each of which represents a potentially important drug target.

The sequence similarity between MAD - 3 and pp40 includes a casein kinase II and consensus tyrosine phosphorylation site, as well as five repeats of a sequence found in the human erythrocyte protein ankyrin.

«About 2 percent, or nearly 500, of all human genes are dedicated to coding protein kinases and over 50 percent of kinases are linked to various human diseases.»

It is no surprise, then, that having faulty protein kinases may lead to a number of human conditions, including cardiovascular diseases, cancer and diabetes.

In experiments with mouse and human hair follicles, Angela M. Christiano, PhD, and colleagues found that drugs that inhibit the Janus kinase (JAK) family of enzymes promote rapid and robust hair growth when directly applied to the skin.

Kim and colleagues are interested in studying the activation mechanism of protein kinase G I (PKG I) because a mutation of PKG I has been shown to cause thoracic aortic disease, as Kim and his collaborators showed in a previous publication in the American Journal of Human Genetics.

To explore this question, the researchers examined the neurological impact of two enzymes that phosphorylate eIF2alpha, kinases termed PERK and GCN2, in different populations of AD model mice — all of which expressed genetic mutations akin to those carried by humans with AD.

But whether p38 MAP kinase inhibitors could be used to treat arthritis in humans is unclear, she says.

Rho - kinase inhibitor Y - 27632 and hypoxia synergistically enhance chondrocytic phenotype and change the S100 protein profile in human chondrosarcoma cells

↵ 3 The abbreviations used are: HUVEC, human umbilical vein endothelial cell; DiI - Ac - LDL, 1,1 ′ - dioctadecyl - 3,3,3 ′, 3 ′ - tetramethyl - indocarbocyanine acetylated low - density lipoprotein; FACS, fluorescence - activated cell sorting; FGFR, fibroblast growth factor receptor; Flk, fetal liver kinase; ICAM, intercellular adhesion molecule; mAb, monoclonal antibody; PE, phycoerythrin; TNF, tumor necrosis factor; VCAM, vascular cell adhesion molecule.

For human controls these were Excision repair cross-complementing group 3 (ERCC3), Amplified in osteosarcoma (OS - 9), and Casein kinase - 1d (CSNK1D).

The Monash BDI researchers worked in collaboration with Canadian - based company Kinexus, and used an antibody microarray to simultaneously investigate hundreds of factors involved in cell signalling that were modulated by HCV replication, including human protein kinases.

Rho - kinase inhibitor Y - 27632 increases cellular proliferation and migration in human foreskin fibroblast cells

This thesis primarily investigates the effects of mechanical cyclic stretching, a 5 % low oxygen atmosphere and the Rho - kinase inhibitor, Y - 27632, on protein responses in chondrocytic human chondrosarcoma (HCS - 2 / 8) cells.

In 2005, Cagan's team created a general fly model of a human thyroid tumor caused by mutations in the Ret receptor tyrosine kinase gene, then screened a panel of drugs including a kinase inhibitor called vandetanib that suppressed the tumor (Cancer Res, 65:3538 - 41, 2005).

In their latest research, the Joslin team performed many experiments to explore the actions of these two proteins, called centromere protein A (CENP - A) and polo - like kinase - 1 (PLK1), in mice and in cells from humans and mice.

Clough BH, Zeitouni S, Krause U, et al., Rapid Osteogenic Enhancement of Stem Cells in Human Bone Marrow Using a Glycogen ‐ Synthease ‐ Kinase ‐ 3 ‐ Beta Inhibitor Improves Osteogenic Efficacy In Vitro and In Vivo.

Effect of Saturated Stearic Acid on MAP Kinase and ER Stress Signaling Pathways during Apoptosis Induction in Human Pancreatic ß - Cells Is Inhibited by Unsaturated Oleic Acid.

The human protein kinase HIPK2 phosphorylates and downregulates the methyl - binding transcription factor ZBTB4.

(h) Human phospho - receptor tyrosine kinase array for EphA2, EphB2 and EphB4 ephrin receptors from control BP (shCtr) and BP silenced for Tie2 (siTie2 I and siTie2 II).

Platelet - activating factor - mediated endosome formation causes membrane translocation of p67phox and p40phox that requires recruitment and activation of p38 MAPK, Rab5a, and phosphatidylinositol 3 - kinase in human neutrophils.

Miniaturizing each individual experiment allows us to print whole libraries on a single plate, for instance gRNA / siRNA targeting all kinases in the human genome are printed on approximately 2.06 cm2 (0.32 sq. in.).

Cellular entry of human papillomavirus type 16 involves activation of the phosphatidylinositol 3 - kinase / Akt / mTOR pathway and inhibition of autophagy.

Hunter and his colleague Gerard Manning discovered that the human genome contains quite a few dead kinases whose sequences have nonetheless been carefully preserved.

Human phospho - receptor tyrosine kinase array was performed according to the manufacturer's instructions (R&D, #ARY001B).

Molecular interaction and functional regulation of ClC - 3 by Ca2 + / calmodulin - dependent protein kinase II (CaMKII) in human malignant glioma.

Anastellin, a fragment of the first type III repeat of fibronectin, inhibits extracellular signal - regulated kinase and causes G1 arrest in human microvessel endothelial cells.

The protein kinase B - RAF is mutated in approximately 8 % of human cancers.

Pim - 3, a Proto - Oncogene with Serine / Threonine Kinase Activity, Is Aberrantly Expressed in Human Pancreatic Cancer and Phosphorylates Bad to Block Bad - Mediated Apoptosis in Human Pancreatic Cancer Cell Lines

Confocal images of P150 dystrophic retina transplanted with hNPCctx — GDNF and double stained with antibodies against human nuclear antigen (red) and either (A) recoverin, a photoreceptor and cone bipolar cell marker (green), or (B) protein kinase Cα (PKCα), a bipolar cell marker (green).

I wanted to inactivate the gene BRAF (a kinase implicated in several human cancers) in A549 cells (a human lung cancer cell line), armed only with viruses obtained through Addgene's viral service and the methods sections of scientific articles (gasp).

Currently, 31 tyrosine kinase inhibitors are FDA approved for human therapy, with many more in clinical trials.

(a kinase implicated in several human cancers) in A549 cells (a human lung cancer cell line), armed only with viruses obtained through Addgene's viral service and the methods sections of scientific articles (gasp).

Enhanced Expression of Myristoylated Alanine — Rich C Kinase Substrate (MARCKS) in Airway Epithelium of an Animal Model of Allergic Airway Inflammation and Human Patients with COPD

Inactivation of ABL kinases inhibited the expression of the TAZ target gene AXL, which shows increased expression in several human cancers and correlates with poor prognosis, increased invasiveness and metastasis, and enhanced drug resistance (53, 54).

A self - inactivating lentiviral construct containing a mouse phosphoglycerate kinase - 1 internal promoter driving the human gene encoding GDNF [44] was used to generate GDNF - secreting hNPCctx.

ABL tyrosine kinases play an oncogenic role in human leukemias (4, 5) and promote the progression of solid tumors (5, 6).

University of Wisconsin A chemical - genetic library of human cell lines for evaluating pharmacologic inhibition of protein kinases

Flavopiridol inhibits several cellular kinases and has demonstrated cytostatic and cytotoxic activity in vitro and in vivo in numerous human tumor cell lines and xenograft models (including human breast, prostate, and lung carcinoma) at clinically achievable concentrations.

Prediction of the structure of human Janus kinase 2 (JAK2) comprising the two carboxy - terminal domains reveals a mechanism for autoregulation

At that time researchers had only just begun to understand the 58 tyrosine kinases in the human proteome, of which EGFR is one.