They found an average quantum efficiency of approximately 30 per cent — very close to that of
human rod cells estimated from behavioral experiments.
Not exact matches
As it turns out, the missing link was a previously unknown type of light - sensitive
cell in the
human eye, distinct from the familiar
rods and cones that are responsible, respectively, for night and color vision.
The research, published this week in the Proceedings of the National Academy of Sciences USA (PNAS), indicates that
rod - shaped particles (150 nanometers in diameter by 450 nanometers long) penetrated
human cells about four times faster and traveled farther into the
cells than particles with more balanced dimensions (such as 200 nanometers by 200 nanometers).
Krivitsky notes that
rod -
cell efficiency is comparable to the quantum efficiencies of state - of - the - art
human - made single - photon detectors such as photomultipliers (40 per cent) and avalanche photodiodes (50 per cent); remarkably,
rod cells occupy an area of only 5 by 50 micrometers and contain their own power supply.
Rod and cone
cells in the
human retina contain proteins called opsins that change shape when light strikes them.
The location of transplanted
human cells, their expression profile and ability to phagocytose
rod photoreceptor material was examined in vivo using immunohistochemistry.
To further examine the morphology of
cells and the localization of protein expression within the retina, immunohistochemical staining of both paraffin and OCT retinal sections was performed with the following antibodies (Table S1):
human cone arrestin (for cone photoreceptors), rhodopsin (for
rod photoreceptors), RPE65 (for the retinal pigment epithelium, RPE), glial fibrillary acidic protein (GFAP, for astrocytes and Müller
cells), glutamine synthetase (for Müller
cells) and G0alpha (for ON bipolar
cells).