We combine it with the use of
human tissue culture cells in which we validate some of the results obtained in egg extract.
Ralf Kittler (Buchholz, MPG)-- «Functional genomic analysis of cell cycle progression in
human tissue culture cells» (2006)
At the time, his varied interests — in the use of skin cell culture to treat burns, in
human tissue cultures, and in biopharmaceutical production — led him to do his final year, 6 - month project on culture in a bioreactor.
Lumsden and his team tested the new nanoparticles in
human tissue cultures, observing where the tPA arrived and exactly how long it took to destroy blood clots.
Not exact matches
Scientists are forced either to experiment on whole animals, which is expensive, raises ethical issues and may not predict effects in
humans, or to perform tests on microscopic
human cells found in
tissue cultures, which have been altered to live forever and bear little relation to actual living, breathing people.
Some of the leading non-animal tests are conducted on cell
cultures,
human and animal corneas from eye banks, corneal
tissue cultures, and frozen corneas supplied by hospitals.
Pseudomonas also will kill
human lung cells in
tissue culture dishes.
As it can take weeks to grow
human cells into intact differentiated and functional
tissues within Organ Chips, such as those that mimic the lung and intestine, and researchers seek to understand how drugs, toxins or other perturbations alter
tissue structure and function, the team at the Wyss Institute for Biologically Inspired Engineering led by Donald Ingber has been searching for ways to non-invasively monitor the health and maturity of cells
cultured within these microfluidic devices over extended times.
It is unclear whether shrinking telomeres will be problematic in
culturing human tissue transplants, but if it turns out they are, Geron has an answer for that too.
In rats and
tissue cultures of
human nerve cells, these «beta sheet breakers» not only prevent amyloid plaques from forming, but also dissolve existing plaques.
In studies with
human fibroblasts that make up connective
tissue, Boger's team tested whether NOD1 activity could affect CMV replication in
cultures of cells grown in the lab.
To test their hypothesis, the researchers harvested dermal papillae from seven
human donors and cloned the cells in
tissue culture; no additional growth factors were added to the
cultures.
The main reason that rodent hair is readily transplantable, the researchers suspected, is that their dermal papillae (unlike
human papillae) tend to spontaneously aggregate, or form clumps, in
tissue culture.
While there, he worked with cell
cultures derived from
human fetal
tissue.
But Stanford University neuroscientist Sergiu Paşca has crafted a method for making and maintaining 4 - millimeter - wide balls of
human cortex — like
tissue (he calls them spheroids) in 3 - D
culture for an extended time.
protected animals»); studies on in vitro systems (whole perfused organs,
tissue slices, cell and
tissue cultures, and subcellular fractions); and
human studies (including estimations of occupational and environmental exposure, postmarketing surveillance, epidemiology, and the ethical and strictly controlled use of
human volunteers).
One postdoc presents data on her efforts to develop an organoid model for small - cell lung cancer; another reports progress on
culturing hormone - secreting organoids from
human gut
tissue.
Prior research with
cultured tissue had shown that a mix of chemicals could change bone marrow stem cells from mice to those resembling brain cells, but when a team led by neurologist Lorraine Iacovitti of Thomas Jefferson University in Philadelphia tried the same brew on
human cells, the number altered was modest.
When they added these proteins to
cultures of brain
tissue from aborted
human fetuses, the
tissue formed folds, as it does in
human fetuses at about 20 weeks of gestation.
They confirmed low levels of miR - 184 expression in
human glioma
tissue samples and
cultured cell lines as well as an increase in the expression of SND1 compared to normal brain
tissue.
Then, by placing surface proteins of each HCV virus onto these pseudoviruses, the researchers were able to efficiently infect
human cells with the HCV strains in
tissue culture.
«Our group pioneered the development of cell
culture technology for harvesting large numbers of stem cells from
human bone marrow and
human umbilical cord blood,» Dr. Yeh said, noting that stem cells from these two sources are abundant and can be guided into different types of cells using
tissue engineering.
We're now exploiting these ideas in the construction of high performance electronic scaffolds for actively guiding and monitoring growth of
tissue cultures, and networks for 3D electronic systems that can bend and shape themselves to the organs of the
human body.
In short, this technique involved
culturing human fibroblasts from scar
tissues in a petri dish.
All
human cell lines growing in
tissue culture dishes, and about 95 % of cells in a
human being, split evenly, resulting in daughter cells of equal size.
«The region selective - state of these stem cells is entirely novel for laboratory -
cultured stem cells and offers important insight into how
human stem cells might be differentiated into derivatives that give rise to a wide range of
tissues and organs,» says Jun Wu, a postdoctoral researcher in Izpisua Belmonte's lab and first author of the new paper.
Comparison of
Human alveolar osteoblasts
cultured on polymer - ceramic composite scaffolds and
tissue culture plates.
Because of the lack of suitable
tissue culture or animal models, studies to track their environmental sources and their pathogenesis in
humans and identify emergent strains have been difficult.
The team found that DHEA speeds up the circadian rhythm in
human cells, as well as in
cultured cells and
tissues of mice.
7/16/2008 Improved
Culture System for Hepatitis C Virus Infection A University of California, San Diego School of Medicine researcher has developed the first tissue culture of normal, human liver cells that can model infection with the Hepatitis C virus (HCV) and provide a realistic environment to evaluate possible...
Culture System for Hepatitis C Virus Infection A University of California, San Diego School of Medicine researcher has developed the first
tissue culture of normal, human liver cells that can model infection with the Hepatitis C virus (HCV) and provide a realistic environment to evaluate possible...
culture of normal,
human liver cells that can model infection with the Hepatitis C virus (HCV) and provide a realistic environment to evaluate possible... More...
Researchers had developed the technologies needed to create organoids years before — how to grow cells in
culture, how to isolate stem cells from
human tissue, and how to coax the stem cells, undifferentiated and immature, to become specific types of cells at later stages of development.
Now, instead of dropping a dollop of shampoo in a rabbit's eye to check for an allergic reaction, the shampoo goes into a dish containing
cultured human cells or artificial skin
tissue.
Human astrocytes were purchased from Sciencell Research Laboratories (isolated from human cerebral cortex, Cat # 1800, Carlsbad, CA) and were cultured in human astrocyte medium (Sciencell, Cat # 1801) on poly - L - lysine coated tissue culture di
Human astrocytes were purchased from Sciencell Research Laboratories (isolated from
human cerebral cortex, Cat # 1800, Carlsbad, CA) and were cultured in human astrocyte medium (Sciencell, Cat # 1801) on poly - L - lysine coated tissue culture di
human cerebral cortex, Cat # 1800, Carlsbad, CA) and were
cultured in
human astrocyte medium (Sciencell, Cat # 1801) on poly - L - lysine coated tissue culture di
human astrocyte medium (Sciencell, Cat # 1801) on poly - L - lysine coated
tissue culture dishes.
In
tissue culture the wild oregano oil virtually completely destroyed the
human coronavirus, a 99.9 % kill, in a mere 20 minutes.
A
tissue culture study of
human breast - cancer cells found that epigallocatechin gallate, or EGCG, an active compound in green tea, protects against some forms of breast cancer by regulating estrogen receptors on breast cells and inhibiting growth and reproduction of estrogen - dependant breast - cancer cells.
A flavorful component of cinnamon oil, called cinnamaldehyde, may help prevent colon cancer, according to a
tissue culture study of
human colon cancer cells published in the May 2010 issue of the journal «Molecules.»
Microscopy
Tissue Culture Multi-Color Flow Cytometry Assay Development High - Content Imaging Protein Expression & Purification Techniques Molecular Genetics Techniques Western Blot ELISA Immunofluorescence Microscopy
Human Primary Cell
Culture Data Analysis