Sentences with phrase «hybridization at»

Still, hybridization at AMG is something to look forward to, as Moers promises more power and better lap times from its cars.

(B — C) aplnrA; aplnrB double heterozygote parents were incrossed and their progeny examined by in situ hybridization at 50 % epiboly.

After acetylation by treatment with 0.1 m triethanolamine 0.25 % acetic anhydride, pH 8.0, the tissue was prehybridized (prehybridization solution: 0.75 m NaCl, 25 mm PIPES, 25 mm EDTA, 5 × Denhardt's reagent, 0.2 % SDS, 250 μg / ml Escherichia coli tRNA, and 500 μg / ml salmon testis DNA, pH 6.8) at room temperature for 5 h. Riboprobes were incubated for 10 min at 85 °C before application onto the sections and used for hybridization at a final concentration of 500 ng / ml.

Not exact matches

Although Aston Martin hasn't actually revealed official performance figures, power is expected to register at more than 1,000 horsepower thanks to a 6.5 - liter V - 12 engine and some form of hybridization.

«At last, we know there was a hybridization, possibly a chance cross» between Cabernet franc and Sauvignon blanc.

Yoamel Milián - García, a biologist at the University of Havana, first published data on the hybridization epidemic in 2015.

And the outcomes «may alter the view of hybridization in animals as some form of mistake,» comments Loeske Kruuk of the University of Edinburgh, «because it is now clear that we can not take the consequences for fitness at face value.»

«We weren't clear at the time if [hybridization] was something weird about swordtails,» she says, «but it's becoming clear that many species groups are that way.»

Therefore, the results achieved in this study have shown that nonlinear effects in graphene nano - mechanical resonators reveal a hybridization effect at high energies that, if controlled, could open up new possibilities to manipulate vibrational states, engineer hybrid states with mechanical modes at completely different frequencies, and to study the collective motion of highly tunable systems.

«The big question for conservation of polar bears is if hybridization occurs rapidly and in combination with other stressors, will that hybridization have more of a negative effect now than it did in the past,» says Andrew Whiteley, a geneticist at the University of Massachusetts, Amherst.

These celibate species resulted from the hybridization of different sexual species, a process that instills the parthenogenetic lizards with a great amount of genetic diversity at the outset.

Not only was intra-specific gene flow among populations seen to be minimal at that time, but, somewhat incongruously, inter-specific hybridization, or the movement of genes among species, was seen to be a much larger force in evolution than intra-specific allele movement.

In some cases, hybridization can lead to reduced genetic diversity in animals, according to David Tallmon, an associate professor of biology at the University of Alaska.

The authors also noted that continued hybridization may still occur, although at low levels.

The study showed at least three instances of introgressive hybridization between the two species.

This allowed the group led by Ole Seehausen (head of the Fish Ecology and Evolution department at Eawag and Professor of Aquatic Ecology at Bern University) to provide strong evidence for his theory that hybridization between divergent species, in conjunction with ecological opportunity, can facilitate rapid adaptive radiation.

In situ hybridization on sections of gustatory ganglia (geniculate, petrosal / nodose) and a somatosensory ganglion (trigeminal) were performed to reveal serotonin receptor gene expression at the cellular level.

Hybridization was performed overnight at 56 °C in a chamber humidified with 50 % formamide.

«Why is it that biologists in general who look at mammalian genomes haven't found hybridization?

We sampled the bees from the surface of the bee ball at 0, 30, and 60 min after formation of the bee ball and examined the Acks - expressing brain regions using in situ hybridization (n = 5, 7 and 7 for 0, 30 and 60 min, respectively).

RNA extraction, probe synthesis and purification, chip hybridization, and scanning were carried out at SUNY - MAC (Syracuse, New York).

(D — R) In situ hybridization of Acks in each brain area shown in (B) in the brains of workers at 0 (D, G, J, M, and P), 30 (E, H, K, N, and Q) and 60 min (F, I, L, O, and R) after the bee ball formation.

When in situ hybridization was performed with sections corresponding to the middle (Figure 4, Figure S1, and Figure S2), more rostral (Figure S3) and more caudal brain parts (Figure S4), the Acks signals were most densely detected in the Class II KCs at 30 and 60 min after the bee ball formation (Figure 4H, I, K, and L, Figure S3H, I, K, and L, and Figure S4H, I, K, and L), whereas they were scarcely detected at 0 min (Figure 4G and J, Figure S3G and J, and Figure S4G and J).

The riboprobes were diluted in hybridization buffer (50 % formamide, 10 mM Tris - HCl, 200 µg / ml tRNA, 1 × Denhardt's solution, 10 % dextran sulfate, 600 mM NaCl, 0.25 % SDS, 1 mM EDTA at pH 7.6), heat - denatured at 85 °C for 10 min, and then added to each slide.

Statistical analyses of the number of Acks signals detected in in situ hybridization experiments using sections corresponding to the middle brain parts (n = 5, 5, and 4 for 0, 30, and 60 min, respectively) revealed that there was a significant increase in the number of Acks signals at 30 and 60 min after the bee ball formation in both the Class I and the Class II KCs, and at 60 min after the bee ball formation in the brain area between the DLs and the OLs, and in the OLs (Figure 4S).

(D — R) In situ hybridization of Acks in each brain area shown in (B) in the brains of workers at 0 (D, G, J, M, and P), 30 (E, H, K, N, and Q), and 60 min (F, I, L, O, and R) after bee ball formation.

Plants used for scanning electron microscopy, RNA - seq, in situ hybridization, metabolic profiling, and PCZ treatment were grown in a high - light growth chamber under short - day conditions (31 °C / 22 °C [day / night], 12 h light / 12 h dark, 50 % relative humidity, chamber outfitted with 6 T8 fluorescent 5 - foot bulbs per 10 - square - foot canopy, light intensity at 200 µmol m − 2 s − 1 PAR) at the Danforth Center's growth facility.

One of the most striking aspects of this study is that hybridization between two distinct species led to the development of a new lineage that after only two generations behaved as any other species of Darwin's finches, explained Leif Andersson, a professor at Uppsala University who is also affiliated with the Swedish University of Agricultural Sciences and Texas A&M University.

The hybridization was performed at 50 °C overnight in 50 % formamide buffer [0.5 ng / mL probe, 1 mg / mL tRNA, 0.1 mg / mL poly (A), 30 mM DTT, 0.3 M NaCl, 10 mM Tris - HCl, 1 mM EDTA, 10 % dextran sulfate, and 1 × Denhardt's solution].

The team's work highlights this by presenting a comprehensive view of genome evolution on many different levels (e.g., differences in ploidy, aneuploidy, genetic variants, hybridization, and introgressions) that is difficult to obtain at the same scale and accuracy for other eukaryotic organisms.

Hybridizations were performed overnight at 58 °C using standard protocols [56].

Following hybridization, coverslips were removed, and slides were washed in 0.4 × SSC / 0.3 % NP - 40 (Abbott Molecular, Inc.) at 73 °C for 2 min and then in 2 × SSC / 0.1 % NP - 40 (Abbott Molecular, Inc.) for 1 min at room temperature.

In preparation for in situ hybridization, tissue specimen slides at room temperature were deparaffinized by soaking in three changes of Hemo - De solvent (Scientific Safety Solvents, Keller, TX) for 5 min each, followed by two 1 - minute rinses in 100 % ethanol, an incubation in a solution of 45 % formic acid (Fisher) / 0.3 % hydrogen peroxide (Calbiochem) for 15 min, and a rinse in water for 3 min.

Considering the many potential culprits at play, be it wheat hybridization, gluten, other wheat proteins, FODMAPs or glyphosate contamination, it's not surprising that wheat and other grains cause such problems for so many.

And it turns out, despite hundreds of hybridizations of durum wheat in the past century, its gluten content is still approximately the same as it was, at the turn of the century.

There are lots of other changes this represents too, such as the presence of chemicals in our food supply (pesticides, herbicides, xenoestrogens, etc) that didn't exist in the ancient diet, as well as the dramatic changes caused by hybridization of many of our foods, which creates versions of fruits and vegetables bred more for size, sweetness, and appearance as opposed to natural nutrition... this aspect has decreased micronutrient content in the modern day food supply (just look at wild blueberries vs cultivated blueberries as an example of that with wild berries coming in at more than DOUBLE the antioxidants from ORAC testing).

At the dawn of a new era devoted to downsizing, hybridization, and electromobility, V - 8s will soon be condemned as fossils from a wasteful past — so get one like the charismatic, turbocharged direct - injection, dry - sump 32 - valve 3,982 cc unit that is the essence of the AMG GT S while you can.

Anyway, speaking to Autocar, Andy Palmer — engineering, sales and marketing boss at Nissan — said that there is an «inevitability» about electrification of all future cars and that the new Nissan GT - R «will see some form of hybridization».

But at least the mileage with hybridization, is respectable.

According to Wikipedia, there are at least seven breeds of wolfdog hybridization.

The Pleasure Principle, the current exhibition at FARAGO, takes this hybridization to even further heights.

When he was twenty, he studied horticulture at the University of Versailles, where he began thinking about concepts such as grafting and hybridization, and became fascinated by the premise of conceptual art.

At a time when the genetic modification of foods is a genuine threat to human well being, Brooklyn - based artist Naomi Campbell has created a hybridization of her own, merging her scientific interests with a creative investigation of genetics and technology.

The dimensions of such a museum, in a state of permanent reconfiguration and hybridization, are impossible to measure, far greater than its actual floor plan or elevation, being at any one time a gallery, a laboratory, a studio, a workshop, an academy, a theater, a fiction.

Not formally trained in art, Lavier studied at the School of Horticulture in Versailles, where he discovered and deepened his interest in hybridization.

This exhibition looks at two facets of Pindell's practice that have remained consistent through five decades of artmaking: abstract paintings and constructions on canvas, paper, and board; and a body of work Pindell calls «video drawings», an ongoing series of photographic prints that arise from her unique hybridization of photography, video, and drawing.

Regardless of whether hybridization rates may increase, at least for polar bears any effect they might have on population welfare pale by comparison to the loss of habitat and subsequent loss of foraging ability.

But some time ago, we had this discussion, and the chemists described what they called s - p hybridization, and claimed that in fact the CO2 molecule looks like O = C =O or at right angles would be O - C - O, seeing the bond pair edge on.

Anyway it's linear, if you want to think of it in terms of sp hybridization the carbon has two sp orbitals (at 180º) forming sigma bonds with two pi bonds formed by the orthogonal p - orbitals.

Executive chairman Eric Schmidt appeared to hint at the hybridization in his keynote at the 2011 Mobile World Congress in Barcelona.