Chromosomal position of the genomic regions targeted with
the hybridization capture on microarrays.
For each patient, we designed a custom
hybridization capture array (Nimblegen) targeting all candidate somatic events from the primary tumor and relapse sample (median: 539 per case).
Therefore, in 2015, the DNA was extracted from the teeth and, following
hybridization capture of the mitochondrial and Y chromosome fractions, sequenced by a next generation method.
Here we show that
hybridization capture on microarrays can successfully recover more than a megabase of target regions from Neandertal DNA even in the presence of ~ 99.8 % microbial DNA.
Not exact matches
The Research Histology Core Laboratory includes specialized equipment for immunohistochemistry, fluorescent in situ
hybridization FISH, laser
capture microdissection, and tissue microarray construction.
It was a fortuitous time to be in his lab because the method of molecular
hybridization had just emerged from the work of Spiegelman, where radiative probes are hybridized to DNA
captured on nitrocellulose filters.
In
hybridization or
capture - based target enrichment of samples prior to next - generation sequencing (NGS), pools of biotinylated oligonucleotide probes specific for target regions of interest are hybridized to a sequencing library in solution.