Sentences with phrase «hybridoma supernatant»

Cells were incubated with GCTM2 hybridoma supernatant (mouse IgM) for 30 min at room temperature following by three washes with 100 mM Tris - HCl, pH 8.5.

1 ml of antibody - containing hybridoma supernatant is only $ 40, significantly cheaper than commercially available antibodies.

After blocking for 1 hour with 10 % normal goat serum (NGS), the slides were incubated with 200 μl primary antibody, either a polyclonal rabbit anti-Borrelia (Accurate chemicals, Westbury, NY) at 1:200 or the anti-OspA monoclonal, CB10, with a 1:30 dilution of hybridoma supernatant for 1 hour at room temperature (RT).