The CAM and zebrafish in vivo assays and the ex vivo mouse aortic ring assay clearly
identify kahweol as a new anti-angiogenic compound, but gives no information on which specific steps of angiogenesis are targeted by this compound.
Our results in the zymographic assays for gelatinase and urokinase activities clearly showed that, in fact,
kahweol was able to inhibit the expression of both MMP - 2 and uPA,
identifying them as two relevant molecular targets for
kahweol.