Sentences with word «imidazole»

The pellet was resuspended in 10 ml of resuspension buffer (20 mM NaHPO4, 300 mM NaCl, 20 mM Imidazole, pH 7.8), and cell lysis was induced by freezing at − 80 °C with PMSF, Leupeptin and Pepstatin added to inhibit protease activity.

This screening tool led to the discovery that a small molecule with a long name, 2 - acetyl4 (5)- tetrahydroxybutyl imidazole, or THI for short, blocks an enzyme that breaks down sphingosine 1 - phosphate.

Lithographically patterned Pd nanowires were simply overcoated with a Zn - based zeolite imidazole framework (ZIF - 8) layer composed of Zn ions and organic ligands.

The imido - acid salts are a version of the organic molecules patented by Bara and UA in August 2013 to perform the same function as imidazoles, but with an anionic, meaning negatively charged, group tethered to them.

The harvested tissue culture supernatants (~ 200 mL for each protein) were supplemented with imidazole (10 mM) before loading each well at 1 mL / min.

The plate was washed with 1.6 mL of binding buffer and proteins eluted with 0.2 mL of elution buffer (20 mM sodium phosphate, 0.4 M imidazole, 0.5 M NaCl, pH 7.4).

Johnson JC, Martinez O, Honko AN, Hensley LE, Olinger GG, Basler CF. Pyridinyl imidazole inhibitors of p38 MAP kinase impair viral entry and reduce cytokine induction by Zaire ebolavirus in human dendritic cells.

Briefly, a 96 - well His MultiTrap HP filter plate (GE Healthcare) was pre-equilibrated with binding buffer (20 mM sodium phosphate, 40 mM imidazole, 0.5 M NaCl, pH 7.4) at a flow rate of 1 mL / min.

Electrochemical Investigation of Corrosion on AISI 316 Stainless Steel and AISI 1010 Carbon Steel: Study of the Behaviour of Imidazole and Benzimidazole as Corrosion Inhibitors

And the company has teamed up with industrial giant 3M to produce swaths of their imidazole membranes in a reel - to - reel process.

Incorporation of the imidazole ring into compound 152 resulted in a weakly active compound.

The researchers developed a new methodology for synthesizing this imidazole form that requires only eight steps.

However, the presence of an HN13 — HC8 ROE coupled with the absence of an HN13 — CH10 ROE suggests that rotation about the C9 — N13 bond is more restricted, which may force the imidazole ring of RIPK2 inhibitor 1 into a position opposite to the corresponding CF3 group of ponatinib - RIPK2 (Fig. 1B, panel iii), which would place it in a more solvent - exposed position than the buried position of the CF3 group.

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).

It has an imidazole functional group, which is typical for an aromatic amino acid.