Sentences with phrase «immunoblot analysis»
"Immunoblot analysis" refers to a laboratory technique used to detect and identify specific proteins in a sample. It involves separating proteins based on their size using a gel and then transferring them onto a membrane. This membrane is then exposed to antibodies that bind to the specific protein of interest, making it visible and allowing scientists to study and analyze the protein. Full definition
Immunoblot analysis of recombinant antigens and B. burgdorferi lysates with representative sera from mice inoculated by i.p. / s.c. routes with B31 - A3 WT, ospC7 mutant, and ospC7 / ospC +4 complemented B. burgdorferi clones.
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Levels of phosphorylated JAK2 (p - JAK2), total JAK2, phosphorylated STAT3 (p - STAT3), total STAT3 and actin were analyzed by immunoblot analysis.
Immunoblot analysis using mouse monoclonal antibody to human IgM detected IgM to cytomegalovirus (CMV)- specific proteins (150, 42, 38, 32, and 28 kDa) in 74 (38 %) of 197 seropositive serum samples from 197 individuals in three subject groups: 43 surgical patients, 31 patients with solid tumors, and 123 healthy individuals.
For immunoblot analysis, 50 μg protein from each total cell lysate was electrophoresed through 10 % SDS - polyacrylamide gels, then transferred to PVDF membranes (Bio-Rad).
Cell lysates were analyzed for MEK1, phosphorylated ERK1 / 2 (p - ERK1 / 2), total ERK1 / 2 and actin by immunoblot analysis.
2 days after release of UO or at the corresponding time point in the non-UO sham - operated mice, the left kidneys were harvested and subjected to immunoblot analyses of HIF - 2α and co-detection of TBP as a loading control
Protein was abolished as shown by Northern blot and immunoblot analysis.
Cell lysates were harvested and subjected to immunoblot analysis for MEK1, MEK2, MKK3, MKK6, phosphorylated ERK1 / 2 (p - ERK1 / 2), total ERK1 / 2 (t - ERK1 / 2), phosphorylated p38 (p - p38), total p38 (t - p38) and actin.
Cell lysates were subjected to immunoblot analysis for phosphorylated ERK1 / 2 (p - ERK1 / 2), total ERK1 / 2, phosphorylated p38 (p - p38), total p38 and actin.
Levels of MEK1, MEK2, MKK3, MKK6 and actin were analyzed by immunoblot analysis.
(A) Immunoblotting analysis of Rat1 - DD cells expressing vector, wild - type (WT) allele of human MELK, or an oncogenic allele of PIK3CA (H1047R).