Sentences with word «immunoblotting»

Synaptophysin in (f) by immunoblot by in brain homogenates; for regional effects on synaptophysin and MAP2, analyzed in serial sections by immunohistochemistry, see Fig. 8 in [22]-RRB-.
HuH - 7 cell extracts (lane 3) or FLAG - Sp1 - containing cell extracts (lane 4; 1.5 mg each) were immunoprecipitated with anti-FLAG antibody — conjugated beads and the immunoprecipitates were analyzed by SDS - PAGE followed by immunoblotting with anti-FLAG antibody or anti-DDX3 antibody.
For immunoblot analysis, 50 μg protein from each total cell lysate was electrophoresed through 10 % SDS - polyacrylamide gels, then transferred to PVDF membranes (Bio-Rad).
Reacts in Immunoblot with monkey antiserum to SIV and with Mabs to SIV p27
Immunoblots showed almost complete inhibition of mTOR expression (as shown in Fig. 4B) that resulted in decreased cell proliferation as assessed by MTS assay (A).
After extensive washing with immunoprecipitation lysis buffer, the immunoprecipitated proteins were analyzed by immunoblotting using specific antibodies against DDX3 and FLAG epitope (ab1257, Abcam, Cambridge, United Kingdom).
These Lipoprint data were complemented by the 2 - dimensional apoA - I immunoblot (eFigure 3 in the Supplement) showing elevated levels of prebeta - 1 HDL.
Protein immunoblot and histochemical analysis with antiserum to type I NO synthase suggest that the formation of NO in pancreatic B cells is catalyzed by an NADPH -(reduced form of nicotinamide adenine dinucleotide phosphate), Ca2 + / calmodulin - dependent type I NO synthase of about 150 kilodaltons.
Immunoblotting confirmed all these staining (Fig. 2e and SI Figs. 7c and 13c).
Read the article: An analysis of critical factors for quantitative immunoblotting.
Learn how different detection technologies such as chemiluminescence and fluorescence can affect the quality of immunoblotting data
Western Blotting is an analytical Immunoblotting Technique to detect specific proteins in a cell extract or tissue homogenate.
Distribution of Fc gamma receptors on trophoblast during human placental development: an immunohistochemical and immunoblotting study.
Immunoblotting assays were carried out by standard procedures using Snail (Cell Signaling Technology, Inc.) and vimentin antibodies (Novus Biologicals, Littleton, CO, USA).
(K) Representative immunoblots of 1833 cells transfected with control shRNA, shRNA against ABL1 (shABL1), ABL2 (shABL2), and shRNA # 2 against both ABL1 and ABL2 (shAA # 2) and ABL1 / ABL2 knockdown cells with overexpression of mouse Abl1 / Abl2 (shAA + mAbl1 / Abl2).
(A) Immunoblot illustration of the use of RIPK2 phospho - antibodies in two Hodgkin's lymphoma cells that have constitutive active RIPK2.
(D) Protein carbonyls were detected at different concentrations (2.5, 5, 10 pM) as (DNP) hydrazone adducts via immunoblots with a DNP antibody.
Levels of phosphorylated JAK2 (p - JAK2), total JAK2, phosphorylated STAT3 (p - STAT3), total STAT3 and actin were analyzed by immunoblot analysis.
Cell lysates of hippocampal tissue from the aged huAPP / PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E).
Immunoblots showed decreased levels of p - mTOR and p - p70S6K after treatment with increasing concentrations of rapamycin.
Nondenaturing 2 - dimensional gradient gel electrophoresis followed by apoA - I immunoblotting was performed on plasma from the patient and 17 control participants.20
Proteins from nuclear or cytoplasmic fractions (40 μg) were resolved by 4 % to 20 % SDS - PAGE and transferred onto nitrocellulose membranes for immunoblotting.
In this webinar we will explore the issues of reproducibility in immunoblotting in more depth and discuss how to obtain higher quality data, with a focus on those key factors necessary to create consistent, quantifiable results.
Lysates of primary human tumors were subjected to immunoblotting using the indicated antibodies.
The article summarizes potential inaccuracies with immunoblotting and outlines diagnostic tools to improve precision.
6 May 2015: A Science Signaling paper by Dr. Kevin A. Janes on the challenges of immunoblotting (Western blotting) recently received praise from faulty at Johns Hopkins School of Medicine.
As further evidence of BMP signaling activation, proteins isolated from zebrafish embryos were examined by immunoblotting for phosphorylated Smad1 / 5.
Previous experience with molecular biology techniques (PCR, vector construction, nucleic acid extraction), protein expression, biochemistry (protein purification, enzyme assays, immunoblotting), protein crystallization are highly desirable.
Equal amounts of protein were solubilized in 2.5 x SDS - sample buffer, separated on 12 % SDS - polyacrylamide gels, transferred to Immobulin P and immunoblotted with the antibodies indicated in the Materials section.
Total cell lysates of HuH - 7 cells were incubated with GST or GST - Sp1 fusion protein — prebound glutathione - Sepharose 4B beads and the bound fractions were subjected to immunoblotting with antibody against DDX3 (top).
Immunoblots showed strong expression of HA tag in infected ALCL cells but not in control (uninfected or infected with adeno - β - Gal) cells (A).
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