Sentences with word «immunocytochemistry»

Additional confirmation of knockout and pluripotency was performed using immunocytochemistry (Figure 2, Panel C).
Right eyes were investigated by electron microscopy, left eyes were used for immunocytochemistry and fluorescence light microscopy.
Mitochondrial localization of complex II visualized by immunocytochemistry using ab14715.
Schmidt et al. used high - resolution proteomics, electrophysiology, biochemistry, and immunocytochemistry on wild - type and knockout cells and animals to study PMCA - interacting proteins.
During the 1980s, Marder led studies using antibodies and the technique of immunocytochemistry to reveal the presence of many different neuromodulators in nerve fibres around the STG of crabs, including serotonin, proctolin and GABA.
Transmission electron microscopy with colloidal gold immunocytochemistry showed structural damage in young and aged microvessel endothelium of ApoE4 animals extended to the cytoplasm of perivascular cells, perivascular nerve terminals and hippocampal neurons and glial cells.
To rule out whether this might be due to low targeting efficiencies, we performed immunocytochemistry against OCT3 / 4 and observed an 80 % infection rate (data not shown), suggesting this was not an inability to deliver OCT3 / 4 to hNSCs.
This workshop will showcase a range of real - time, live - cell assays for various cell types and co-culture models, and will introduce brand new reagents and software tools that will enable you to perform live - cell immunocytochemistry and immunophenotyping.
Following perfusions, eyes were either excised, cryoprotected, frozen and cut as described for immunocytochemistry above or the retina was removed intact as a whole - mount preparation.
Immunocytochemistry now indicates that SR is expressed primarily in forebrain glutamatergic neurons with the remainder in GABAergic interneurons.
e) Representative Immunocytochemistry (ICC) images of γH2Ax foci showing dose - dependent increase in DSB foci post treatment with Bleomycin treatment.
d) Immunocytochemistry analysis showing reduced CD44 expression in siTRF2 silenced HCT116 post treatment with Bleomycin sulphate when compared to scrambled transfected HCT116 post treatment with Bleomycin.
Beginning in October 2017, Bio-Techne began to distribute Vector Laboratories products to complement the over 40,000 primary antibodies from Novus Biologicals ® validated for immunocytochemistry and immunohistochemistry.
Immunocytochemistry on the localization of 5 - hydroxytryptamine in monkey and rabbit taste buds
Intracellular expression of human immunoglobulin in HB1.F3.H2IgG NSCs was visualized using immunocytochemistry.
After editing, cells were analyzed for AcGFP1 expression and pluripotency via flow cytometry (Panel B) and immunocytochemistry (Panel C).
After, cells were analyzed by flow cytometry or immunocytochemistry for AcGFP1 and pluripotency markers.
ab134375 staining CD24 in human HeLa cells by ICC / IF (Immunocytochemistry / immunofluorescence).
Ab7291 staining alpha tubulin in human breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).
Immunocytochemistry / Immunofluorescence - Anti-alpha Tubulin antibody [DM1A]- Loading Control (ab7291)
Catecholaminergic abnormalities in the brainstems of infants with SIDS are controversial, with reports of positive and negative findings using immunocytochemistry and tissue autoradiography.26 - 29 Our study does not support a major abnormality in SIDS cases in medullary 5 - HT nuclei that receive projections from rostral catecholaminergic cell bodies in the pons and midbrain.
Immunocytochemistry was performed using standard methods as described previously [27,43].
Immunocytochemistry / Immunofluorescence of mixed neuron - glial cultures labelling rabbit GFAP (red channel) and chicken vimentin CPCA - Vim (green channel).
ab7260 staining GFAP in cells from mouse brain tissue sections by ICC / IF (Immunocytochemistry / immunofluorescence).
We used immunocytochemistry to examine whether the validated miR - 181a target genes were expressed on mDA neurons.
Immunocytochemistry was performed as previously described [17].
Immunocytochemistry of HV cells in 2i culture show the persistence of some Venus cells that have lower levels of Nanog expression, whereas Venus and Oct4 co-express.
Immunocytochemistry was performed essentially as described above for cells.
ab16667 staining Ki67 - Proliferation Marker in human HEp - 2 cells by ICC / IF (Immunocytochemistry / immunofluorescence).
For immunocytochemistry and neurite growth assays, a plating density of 5 × 104 cells / well of a 24 - well plate was used.
ab7260 at 1 / 10000 dilution staining Mouse cortical astrocytes by Immunocytochemistry.
Immunocytochemistry / Immunofluorescence analysis of human cardiac stem cells labeling Ki67 with ab16667 at 1/250 dilution.
The in vivo expression of RPE65 was not detectable by immunocytochemistry.
The antibodies listed above in the immunocytochemistry section were also used on eye sections together with a cocktail of antibodies generated against human - specific markers (HSM) to identify human cells (the Oka blood group antigen, mouse TRA -1-85, 1 ∶ 10 (a kind gift from Peter Andrews, University of Sheffield, Sheffield, UK) together with mouse human nuclear antigen, 1 ∶ 1000, Millipore).
ab2910 staining Caveolin - 1 - Caveolae Marker in HeLa cells by ICC / IF (Immunocytochemistry / immunofluorescence).
ab51608 staining of HIF -1-alpha in untreated HeLa cell line by ICC / IF (Immunocytochemistry / immunofluorescence).
ab51608 staining HIF -1-alpha in HeLa cell line treated with Cocl2 by ICC / IF (Immunocytochemistry / immunofluorescence).
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