One of the most popular models is
the immunodeficient nonobese diabetic severe combined immunodeficiency (NOD scid) gamma (NSG) transgenic mouse line, which can be endowed with a humanized immune system using CD34 + hematopoietic stem cells.
To further validate our result in clinical samples, we obtained primary tumor from patients with advanced breast cancer and the tissue was passaged once in
nonobese diabetic / severe combined
immunodeficient mouse without in vitro culture.