Sentences with word «immunofluorescence»

Olfactory bulb tissue sections of isochronic (control) and heterochronic old (Het - O) mice were stained by immunofluorescence for BrdU (replicating cell marker) and neuronal neurons (NeuN) to detect newborn neurons.

Frozen sections of lung and colon tissues from wild - type mice and Slc6a14 − / − mice were also used for immunofluorescence analysis of Slc6a14 protein expression with an antibody raised against a mouse peptide sequence [17].

Information regarding the primary antibodies utilized in immunofluorescence analysis and primers utilized in RT - PCR, genotyping, and bisulfite sequencing analyses.

Apoptosis and necrosis were quantified using immunofluorescence assays; bioconjugate - treated cells were early apoptotic after 4 hours, proving effective initiation of programmed cell death.

Bulk - cultured PC3 or PC3 - GFP cells, or clone - derived cultures were plated on glass coverslips and used in immunofluorescence staining for CD44, α2β1 integrin, and β - catenin using monoclonal antibodies and protocols described previously (27 — 29).

Examination of tissue sections by immunofluorescence microscopy showed a loss of Cxcr6GFP / + cells from the SCS following FTY720 treatment (Figure 5F).

As a service and in collaboration with Swedish researchers we successfully established multiplex immunofluorescence staining protocols for > 20 different human and rodent tissue types and delivered terabytes of image data to our collaborators.

For a proof of principle test, Deosarkar grew the rat brain blood vessel cells on the vascular side of the chip and tested the barrier they formed under a variety of flow conditions with a range of tests including immunofluorescence staining of tight junctions that interconnected the endothelial cells as well as the electrical resistance across the barrier, and by tracking various sized molecules as they crossed to the tissue side of the model.

The Human Protein Atlas displays high resolution, multicolor images of proteins labeled with immunofluorescence at the single cell level.

Positive FHV - 1 test results (virus isolation, immunofluorescence techniques, and conventional PCR assays) do not differentiate subclinically infected cats from those with clinical disease as a result of FHV - 1 infection.

The image shows bottom side a live Ixodes tick as seen under a confocal immunofluorescence microscope.

Figure 5: Effects of kinase inhibitors on p53 expression as revealed by immunofluorescence imaging.

Latex bead labelling and comparative immunofluorescence analysis of the p62 subunit of the TFIIH was performed as described previously [16,17] using primary mouse embryonic fibroblasts at passages 2 — 5.

Microscopy Tissue Culture Multi-Color Flow Cytometry Assay Development High - Content Imaging Protein Expression & Purification Techniques Molecular Genetics Techniques Western Blot ELISA Immunofluorescence Microscopy Human Primary Cell Culture Data Analysis

Following compound treatment, HMOX1 protein was detected using indirect immunofluorescence.

Subsequently, immunofluorescence labeling is performed with a rat - anti-BrdU primary antibody and a goat - anti-rat-Alexa488 secondary antibody to detect BrdU.

Key Highlight: • Handled flourochrome labeled antibody staining used for flowcytometry, cell proliferation assay, immunofluorescence cell staining and cytokine assays using ELISA.

It is a detail of an image made through immunofluorescence, a technique that attaches fluorescent dye to proteins to make them visible.

«When you're doing immunofluorescence, for instance, if you're using the wrong fixation process or fixative you could really get the wrong results even with a great antibody,» he says.

This multicolor immunofluorescence image shows human endothelial cells being infected by cytomegalovirus.

Following vaccination serum will be taken and an assessment of Plasmodium falciparum (Pf) f - specific antibodies against specific malaria proteins such as the circumsporozoite protein (CSP) PfCSP and whole Pf sporozoites (SPZ) will be determined by enzyme - linked immunosorbent assay (ELISA) and automated immunofluorescence assay (aIFA).

The facility utilizes various immunohistochemical techniques and has developed protocols for complex multiplex immunofluorescence on cryosections (fixed and unfixed) and paraffin embedded human and nonhuman tissue samples.

Optimisation of immunofluorescence methods to determine MCT1 and MCT4 expression in circulating tumour cells.

I would like to perform some study about Immunofluorescence staining of lysosome.

Our 9 tech tips will help you achieve successful Immunofluorescence staining

Treatment with the positive control CoPP results in induction of HMOX1 protein as measured by specific immunofluorescence.

This corroborated with the absence of Slc6a14 protein in colon and lung from knockout mice as evident from immunofluorescence (Figure 1B).

The Western blotting results were further confirmed by in situ immunofluorescence staining.

(A) H&E staining and Ki67, phospho - mTOR and HIF1α immunofluorescence (40 × magnification).

We use structural approaches (i.e. X-ray crystallography, hydrogen - deuterium exchange, multi-angle light scattering), biochemical and biophysical methods (i.e. recombinant assays and reconstitutions, in vitro binding studies, fluorescence - based assays), and cell biology techniques (i.e. immunofluorescence, immunoprecipitations) to study the mechanism of chromatin duplication, and we aim to understand how chromatin duplication affects cellular life and disease development.

Hemagglutination, ELISA, and immunofluorescence antibody tests are available, and PCR is highly sensitive and specific when used on fresh tissue and fluid samples.

Direct immunofluorescence for presence of antigen from smears of conjunctival, tonsillar and genital epithelium, and buffy coat.

I have extensive experience in microbiology, biochemistry, molecular biology, protein interaction and purification, western blotting, immuno - blotting, microscopy [which includes immunofluorescence microscopy (single label and double label), epi - fluorescence, phase contrast, single molecule fluorescence microscopy, PALM FRAP].

Secondary antibodies play an important role in many research applications, including immunofluorescence, immunohistochemistry, Western blot, ELISA, flow cytometry, and more.

When using FPs containing two cysteine residues and a sequence intended to localize them to the secretory pathway, a dark pool that is mislocalized to the ER can be detected with immunofluorescence.

The HepG2 cells were treated with ActD for 6 h, and the p53 expression level was examined by immunofluorescence imaging.

High - throughput profiling of protein targets in single cells using multiplexed immunofluorescence and fluorescent bioimaging.

Patients and Methods: A tissue microarray (TMA) consisting of four biopsy cores from 34 matched pairs of nephrectomy and metastatic sites of clear cell RCC was used to assess PD - L1 expression by quantitative immunofluorescence.