Sentences with phrase «immunofluorescence antibody»
Hemagglutination, ELISA, and immunofluorescence antibody tests are available, and PCR is highly sensitive and specific when used on fresh tissue and fluid samples.
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An immunofluorescence image of a melanoma cell stained with antibodies against the cytoskeleton components indicate their invasion into surrounding matrix.
«When you're doing immunofluorescence, for instance, if you're using the wrong fixation process or fixative you could really get the wrong results even with a great antibody,» he says.
Secondary antibodies play an important role in many research applications, including immunofluorescence, immunohistochemistry, Western blot, ELISA, flow cytometry, and more.
Choosing the correct secondary antibody is crucial for IHC and immunofluorescence.
ImmunoStar's 5 - HT6R Antibody demonstrates significant labeling of rat cortex, amygdala and hippocampus and other areas using immunohistochemistry and immunofluorescence.
Immunocytochemistry / Immunofluorescence - Anti-alpha Tubulin antibody [DM1A]- Loading Control (ab7291)
The immunofluorescence protocol is standardized through antibody concentration and incubation time are variable depending on protein abundance and antibody affinity determined during the test staining.
Information regarding the primary antibodies utilized in immunofluorescence analysis and primers utilized in RT - PCR, genotyping, and bisulfite sequencing analyses.
We further had to test a total of eight different antibodies against phospho - AKT and six different phospho - MAPK antibodies, before being able to detect BDNF signalling by immunofluorescence.
(c)--(f) Immunofluorescence staining using Sox2 antibody.
Antibodies used for immunofluorescence staining included YAP1 from Cell Signaling and TAZ from BD Biosciences.
The antibodies used in this study includes COX - 2 (Cayman Chemicals, Ann Arbor, MI, USA), STAT6 for Western blot (Cell Signaling Technology, Danvers, MA, USA), for immunofluorescence (IF)(BD Pharmingen, San Diego, CA, USA), for ChIP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho - STAT6 (Cell Signaling Technology), p300 for Western blot, (Upstate, Lake Placid, NY, USA), for ChIP (Santa Cruz Biotechnology), normal rabbit IgG (Santa Cruz Biotechnology), mouse immunoglobulin (Ig) G1, κ Ig (BD Pharmingen), glyceraldehyde -3-phosphate dehydrogenase (Advanced ImmunoChemical, Long Beach, CA, USA), Cy3 - conjugated AffiniPure Goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) and PARP (Cell Signaling Technology).
(B) Immunofluorescence staining with antibodies against Sox2, Nestin, Olig2 and Tuj1.
(d — f) Immunofluorescence staining of HFF cells with the antibody against VIMENTIN.
Frozen sections of lung and colon tissues from wild - type mice and Slc6a14 − / − mice were also used for immunofluorescence analysis of Slc6a14 protein expression with an antibody raised against a mouse peptide sequence [17].
Subsequently, immunofluorescence labeling is performed with a rat - anti-BrdU primary antibody and a goat - anti-rat-Alexa488 secondary antibody to detect BrdU.
Bulk - cultured PC3 or PC3 - GFP cells, or clone - derived cultures were plated on glass coverslips and used in immunofluorescence staining for CD44, α2β1 integrin, and β - catenin using monoclonal antibodies and protocols described previously (27 — 29).
By immunofluorescence microscopy, Thy1 - PE - labeled CD3ε + cells were observed in the SCS and in nearby lymphatic sinuses, and few labeled cells were detected within the LN parenchyma, confirming that footpad injection of PE - conjugated antibody led to preferential labeling of lymph - exposed LN cells (Figure 3E).
All cases were analysed for IgA anti-gliadin (AGA) by an ELISA test and those with positive results were tested for IgA anti-endomysium antibody (EMA) using immunofluorescence.
FIV antibodies can be detected using a number of techniques, including enzyme - linked immunosorbent assay (ELISA), western blot, and immunofluorescence (IFA) assays.
Key Highlight: • Handled flourochrome labeled antibody staining used for flowcytometry, cell proliferation assay, immunofluorescence cell staining and cytokine assays using ELISA.