Sentences with phrase «immunofluorescence cell»

Key Highlight: • Handled flourochrome labeled antibody staining used for flowcytometry, cell proliferation assay, immunofluorescence cell staining and cytokine assays using ELISA.

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An immunofluorescence image of a melanoma cell stained with antibodies against the cytoskeleton components indicate their invasion into surrounding matrix.

Learn about the latest approaches to assessing cancer - immune interactions («immune profiling») using FFPE tissue sections, multiplexed immunofluorescence, and cell phenotyping software

The researchers, who released their findings in Cancer Prevention Research Journal, published by the American Association of Cancer Research, used a visualization technique — immunofluorescence — to identify stem cells in the colon of pigs that were fed either a high calorie diet or a standard diet.

Additionally, researchers examined prostate cells — normal and cancerous — using immunofluorescence, allowing them to visualize the distribution of the target molecule and look specifically at centrosomal abnormalities and growth patterns.

Immunofluorescence analysis of prostate cancer cells treated with 15mM flutamide, 35?

Immunofluorescence microscopy reveals the different protein profiles of immature stem cells (coloured pink) and mature stem cells (coloured green).

Left: Immunofluorescence analysis of keratin 14 in keratinocytes derived from pluripotent stem cells.

Middle: Immunofluorescence analysis of TRP1 (melanosomes marker) in melanocytes derived from pluripotent stem cells.

We used immunofluorescence and in situ hybridization to detect specific retinal cell classes.

(E) Xpd dose - dependent reduction of TFIIH in homozygous XpdTTD / TTD, hemizygous XpdTTD / KO, and compound heterozygous XpdTTD / † XPCS and XpdTTD / † XP cells by comparative immunofluorescence of the p62 subunit of TFIIH.

Cells were washed once with PBS and immunofluorescence was documented.

A microscope and its parts, image formation, Köhler illumination, optical aberrations, types of lenses, phase contrast, interference contrast, polarization, fluorescence microscopy, laser confocal microscopy, two - photon confocal microscopy, superresolution microscopy, study of dynamic processes in living cells, immunofluorescence.

Immunofluorescence staining of HeLa cells with purified ab32518 at a working dilution of 1 in 50, counter-stained with DAPI.

ab134375 staining CD24 in human HeLa cells by ICC / IF (Immunocytochemistry / immunofluorescence).

Patients and Methods: A tissue microarray (TMA) consisting of four biopsy cores from 34 matched pairs of nephrectomy and metastatic sites of clear cell RCC was used to assess PD - L1 expression by quantitative immunofluorescence.

Ab7291 staining alpha tubulin in human breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).

Contribution of iPS cells to the gonad was analyzed by EGFP expression and immunofluorescence staining.

Antibodies used for immunofluorescence staining included YAP1 from Cell Signaling and TAZ from BD Biosciences.

The antibodies used in this study includes COX - 2 (Cayman Chemicals, Ann Arbor, MI, USA), STAT6 for Western blot (Cell Signaling Technology, Danvers, MA, USA), for immunofluorescence (IF)(BD Pharmingen, San Diego, CA, USA), for ChIP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho - STAT6 (Cell Signaling Technology), p300 for Western blot, (Upstate, Lake Placid, NY, USA), for ChIP (Santa Cruz Biotechnology), normal rabbit IgG (Santa Cruz Biotechnology), mouse immunoglobulin (Ig) G1, κ Ig (BD Pharmingen), glyceraldehyde -3-phosphate dehydrogenase (Advanced ImmunoChemical, Long Beach, CA, USA), Cy3 - conjugated AffiniPure Goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) and PARP (Cell Signaling Technology).

(d — f) Immunofluorescence staining of HFF cells with the antibody against VIMENTIN.

ab16667 staining Ki67 - Proliferation Marker in human HEp - 2 cells by ICC / IF (Immunocytochemistry / immunofluorescence).

The HepG2 cells were treated with ActD for 6 h, and the p53 expression level was examined by immunofluorescence imaging.

Immunocytochemistry / Immunofluorescence analysis of human cardiac stem cells labeling Ki67 with ab16667 at 1/250 dilution.

Overlaying phase contrast and SSEA3 immunofluorescence images revealed that the SSEA3 expression was detected across the entire cell surface (Figure 1E) and using confocal microscopy we observed that the SSEA3 expression was primarily localized to the cellular membrane (Figure 1F).

Apoptosis and necrosis were quantified using immunofluorescence assays; bioconjugate - treated cells were early apoptotic after 4 hours, proving effective initiation of programmed cell death.

ab1 at 1/200 dilution staining HIF - 1 alpha in human 293FT cells by Immunocytochemistry / Immunofluorescence.

For immunofluorescence, the cells were fixed in 4 % paraformaldehyde / PBS for 20 minutes, washed twice with PBS, and blocked with 4 % goat serum in PBS for 30 min, with all procedures performed at room temperature.

Bulk - cultured PC3 or PC3 - GFP cells, or clone - derived cultures were plated on glass coverslips and used in immunofluorescence staining for CD44, α2β1 integrin, and β - catenin using monoclonal antibodies and protocols described previously (27 — 29).

Immunofluorescence analysis of the two populations, following overnight adherence to exclude analysis of non-viable cells, revealed that > 97 % of the SSEA3 - positive population expressed detectable SSEA3 / 488 fluorescence and 0 % of the SSEA3 - negative population expressed detectable SSEA3 / 488 fluorescence (Figure 2C), demonstrating that the fluorescence activated cell sorting process can purify viable subpopulations of cells from a heterogeneous somatic population.

(C — D) Immunofluorescence staining for (C) TRA -1-60 and (D) TRA -1-81 on HUF1 cells.

High - throughput profiling of protein targets in single cells using multiplexed immunofluorescence and fluorescent bioimaging.

To detect regulatory T cells, we co-stained our sections by immunofluorescence for FoxP3 and CD4 - expressing cells.

The microscope and its components, image formation, microscopy in both transmitted and fluorescent light, Kohler illumination, optical aberrations, objective lens types, phase contrast, interference contrast, polarization, fluorescence microscopy, laser confocal microscopy, two - photon confocal microscopy, super-resolution microscopy, study of dynamic processes in living cells, immunofluorescence.

Cells used for immunofluorescence analysis were fixed immediately following overnight adherence to remove dead and non-viable cells and cells used for transcriptional analysis were cultured for 6 days prior to analCells used for immunofluorescence analysis were fixed immediately following overnight adherence to remove dead and non-viable cells and cells used for transcriptional analysis were cultured for 6 days prior to analcells and cells used for transcriptional analysis were cultured for 6 days prior to analcells used for transcriptional analysis were cultured for 6 days prior to analysis.

By immunofluorescence microscopy, Thy1 - PE - labeled CD3ε + cells were observed in the SCS and in nearby lymphatic sinuses, and few labeled cells were detected within the LN parenchyma, confirming that footpad injection of PE - conjugated antibody led to preferential labeling of lymph - exposed LN cells (Figure 3E).

After binding, cells were washed once (carefully using a 200 μl pipette to remove the medium) with RPMI with 0.5 % BSA solution and adherent cells were analyzed by immunofluorescence microscopy or bright field microscopy for quantification.

In cases of neonatal mortality, the diagnosis typically is made postmortem with virus isolation from fresh lung, liver, kidney, and spleen by cell culture techniques and subsequent identification by PCR and sequencing, transmission electron microscopy, immunofluorescence, or fluorescence in situ hybridization.

Microscopy Tissue Culture Multi-Color Flow Cytometry Assay Development High - Content Imaging Protein Expression & Purification Techniques Molecular Genetics Techniques Western Blot ELISA Immunofluorescence Microscopy Human Primary Cell Culture Data Analysis

Tags for this Online Resume: drug discovery, drug screening, FACS, immunofluorescence, western blotting, ELISA, cell based assay, MSD, cell toxicity assays, protain expression, target validation, cAMP assay, FRET, FLIPR