Sentences with phrase «immunofluorescence microscopy»

After binding, cells were washed once (carefully using a 200 μl pipette to remove the medium) with RPMI with 0.5 % BSA solution and adherent cells were analyzed by immunofluorescence microscopy or bright field microscopy for quantification.
By immunofluorescence microscopy, Thy1 - PE - labeled CD3ε + cells were observed in the SCS and in nearby lymphatic sinuses, and few labeled cells were detected within the LN parenchyma, confirming that footpad injection of PE - conjugated antibody led to preferential labeling of lymph - exposed LN cells (Figure 3E).
Immunofluorescence microscopy for localization of Arabidopsis chloroplast proteins.
Immunofluorescence microscopy was performed using a Zeiss Axioplan fluorescence microscope (Carl Zeiss, Inc., Thornwood, NY) equipped with a 100 - W Hg lamp and narrow band pass excitation filters (Chroma Technology Corp., Brattleboro, VT).
Immunofluorescence microscopy reveals the different protein profiles of immature stem cells (coloured pink) and mature stem cells (coloured green).

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A microscope and its parts, image formation, Köhler illumination, optical aberrations, types of lenses, phase contrast, interference contrast, polarization, fluorescence microscopy, laser confocal microscopy, two - photon confocal microscopy, superresolution microscopy, study of dynamic processes in living cells, immunofluorescence.
Overlaying phase contrast and SSEA3 immunofluorescence images revealed that the SSEA3 expression was detected across the entire cell surface (Figure 1E) and using confocal microscopy we observed that the SSEA3 expression was primarily localized to the cellular membrane (Figure 1F).
The microscope and its components, image formation, microscopy in both transmitted and fluorescent light, Kohler illumination, optical aberrations, objective lens types, phase contrast, interference contrast, polarization, fluorescence microscopy, laser confocal microscopy, two - photon confocal microscopy, super-resolution microscopy, study of dynamic processes in living cells, immunofluorescence.
Other diagnostic methods are available including culture, microscopic agglutination test (MAT), immunofluorescence, darkfield microscopy, other serologic tests, and real - time polymerase chain reaction (PCR).
In cases of neonatal mortality, the diagnosis typically is made postmortem with virus isolation from fresh lung, liver, kidney, and spleen by cell culture techniques and subsequent identification by PCR and sequencing, transmission electron microscopy, immunofluorescence, or fluorescence in situ hybridization.
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