To examine the mechanisms of this down - regulation, thin - section electron microscopy was combined with
immunolabeling by gold - conjugated monoclonal antibodies specific to apCAM.
While pre-adsorption with 5 - HIAA conjugate completely
eliminates immunolabeling, pre-adsorption with conjugates of 5 - HT, 5 - HTP and dopamine had no effect on staining intensity or distribution of stain.
We will construct a detailed 3 - dimensional (3D) map of the neuronal innervation of the AQH drainage structures by imaging either fluorescent protein - expressing neurons, or a subtype with specific
neuronal immunolabeling, using high - resolution confocal microscopy and 3D reconstruction of whole - mounts of the anterior segment of mouse eyes.
Immunolabeling is completely abolished by preadsorption with synthetic rat 5 - HT7 receptor (8 - 23).
Immunolabeling is completely abolished by preadsorption with synthetic rat 5 - HT5A receptor (17 - 34).