The immunoprecipitated chromatin by anti — IRF - 9 antibody was analyzed by PCR using primers specific for RIG - G promoter sequences (+11 to − 288).
It will make use of cutting - edge approaches in vivo and in vitro, including mouse genetic models and next - generation sequencing of RNA and
immunoprecipitated chromatin (RNA - seq and ChIP - seq).
Nagaki et al. [6] used anti-CenH3 antibodies to
immunoprecipitate chromatin (ChIP) comprising DNA bound to CenH3 - containing nucleosomes, confirming that CenH3 is associated with both the CentO repeats and the CRR family of retrotransposons.
Not exact matches
The application of next - gen sequencing to RNA libraries (RNA - Seq),
chromatin immunoprecipitates (CHiP - Seq), bisulfite - treated DNA, and regions of open
chromatin (DNAse - Seq) makes it possible to interrogate many aspects of the genome in high - throughput fashion.