Sentences with phrase «immunoprecipitated using»

HIF - 1 alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5 µg of Mouse monoclonal to HIF - 1 alpha and 50 µl of protein G magnetic beads (+).

HIF -1-alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5µg of Rabbit polyclonal to HIF1 alpha and 50µl of protein G magnetic beads (+).

Not exact matches

Nagaki et al. [6] used anti-CenH3 antibodies to immunoprecipitate chromatin (ChIP) comprising DNA bound to CenH3 - containing nucleosomes, confirming that CenH3 is associated with both the CentO repeats and the CRR family of retrotransposons.

Unpurified ab32518 used to immunoprecipitate IKB alpha from human HeLa whole cell lysate.

Unpurified ab32518 used to immunoprecipitate IKB alpha from rat PC12 whole cell lysate.

After extensive washing with immunoprecipitation lysis buffer, the immunoprecipitated proteins were analyzed by immunoblotting using specific antibodies against DDX3 and FLAG epitope (ab1257, Abcam, Cambridge, United Kingdom).

Tip 6: For detection of immunoprecipitated proteins by Western blotting, without or reduced detection of non-specific artifacts (such as the heavy and light chains of the immunoprecipitating antibody), detect primary antibodies using HRP - conjugated anti-rabbit light chain - specific (L) antibody and HRP - conjugated Protein A instead of traditional HRP - conjugated secondary antibodies.

It will make use of cutting - edge approaches in vivo and in vitro, including mouse genetic models and next - generation sequencing of RNA and immunoprecipitated chromatin (RNA - seq and ChIP - seq).

The immunoprecipitated chromatin by anti — IRF - 9 antibody was analyzed by PCR using primers specific for RIG - G promoter sequences (+11 to − 288).

For this protocol, samples were lysed in 1 × RIPK2 lysis buffer [50 mM Tris (pH 7.5), 0 mM MgCl2, 1 % Triton X-100, 1 mM dithiothreitol, 1 mM EDTA, 1 mM EGTA, with freshly added 1 mM β - glycerophosphate and protease inhibitors (phenylmethylsulfonyl fluoride and aprotinin)-RSB- and immunoprecipitated overnight using 1.5 μg rabbit anti-RIPK2 antibody.

METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.

When an antibody to endogenous SUV39H1 was used for immunoprecipitation, MeCP2 was effectively coimmunoprecipitated; conversely, aHA antibodies to HA - tagged MeCP2 could immunoprecipitate SUV39H1 (Fig. 2G).»