Sentences with phrase «immunoprecipitating orrm1»

ab32518 (purified) at 1/20 immunoprecipitating IKB alpha in HeLa cell lysate (Lane 1).

We have identified another family required for RNA editing by immunoprecipitating ORRM1 and performing proteomic analysis in collaboration with the Van Wijk lab.

Furthermore, the 60S splicing complex contains C proteins, and it can be immunoprecipitated with 4F4.

Significant binding by miR -124-3p to two of the potential target genes was seen, as measured from immunoprecipitated RNA - induced silencing complexes.

Nagaki et al. [6] used anti-CenH3 antibodies to immunoprecipitate chromatin (ChIP) comprising DNA bound to CenH3 - containing nucleosomes, confirming that CenH3 is associated with both the CentO repeats and the CRR family of retrotransposons.

The application of next - gen sequencing to RNA libraries (RNA - Seq), chromatin immunoprecipitates (CHiP - Seq), bisulfite - treated DNA, and regions of open chromatin (DNAse - Seq) makes it possible to interrogate many aspects of the genome in high - throughput fashion.

Immunoprecipitates and cell lysates were separated by SDS — PAGE followed by western blotting.

Unpurified ab32518 used to immunoprecipitate IKB alpha from human HeLa whole cell lysate.

Unpurified ab32518 used to immunoprecipitate IKB alpha from rat PC12 whole cell lysate.

Aβ peptides were immunoprecipitated from rat CSF with the W0 - 2 antibody and further subjected to MALDI - MS analysis.

Immunoprecipitated samples were resuspended in 10 μl of TA (33 % acetonitrile, 0.1 % trifluoroacetic acid) and sonicated in a water bath for 10 min.

HuH - 7 cell extracts (lane 3) or FLAG - Sp1 - containing cell extracts (lane 4; 1.5 mg each) were immunoprecipitated with anti-FLAG antibody — conjugated beads and the immunoprecipitates were analyzed by SDS - PAGE followed by immunoblotting with anti-FLAG antibody or anti-DDX3 antibody.

After extensive washing with immunoprecipitation lysis buffer, the immunoprecipitated proteins were analyzed by immunoblotting using specific antibodies against DDX3 and FLAG epitope (ab1257, Abcam, Cambridge, United Kingdom).

MALDI - MS spectra of immunoprecipitated Aβ peptides from rat CSF.

Tip 6: For detection of immunoprecipitated proteins by Western blotting, without or reduced detection of non-specific artifacts (such as the heavy and light chains of the immunoprecipitating antibody), detect primary antibodies using HRP - conjugated anti-rabbit light chain - specific (L) antibody and HRP - conjugated Protein A instead of traditional HRP - conjugated secondary antibodies.

It will make use of cutting - edge approaches in vivo and in vitro, including mouse genetic models and next - generation sequencing of RNA and immunoprecipitated chromatin (RNA - seq and ChIP - seq).

The immunoprecipitated chromatin by anti — IRF - 9 antibody was analyzed by PCR using primers specific for RIG - G promoter sequences (+11 to − 288).

For this protocol, samples were lysed in 1 × RIPK2 lysis buffer [50 mM Tris (pH 7.5), 0 mM MgCl2, 1 % Triton X-100, 1 mM dithiothreitol, 1 mM EDTA, 1 mM EGTA, with freshly added 1 mM β - glycerophosphate and protease inhibitors (phenylmethylsulfonyl fluoride and aprotinin)-RSB- and immunoprecipitated overnight using 1.5 μg rabbit anti-RIPK2 antibody.

Twelve hours after transfection, cells were treated with or without IFNα for another 24 h. Cell lysates (Input) were then immunoprecipitated (IP) with anti — IRF - 9 antibody followed by Western blot (WB) analysis with anti-STAT2 antibody.

Protein - DNA complexes were then immunoprecipitated overnight with polyclonal Oct4 antibody (sc - 9081, Santa Cruz Biotechnology), a mix of monoclonal Oct4 antibodies (BD Transduction Labs, Santa Cruz Biotechnology, Chemicon), or normal serum controls (Pierce) on a rotator at 4 °C.

HIF -1-alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5µg of Rabbit polyclonal to HIF1 alpha and 50µl of protein G magnetic beads (+).

HIF - 1 alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5 µg of Mouse monoclonal to HIF - 1 alpha and 50 µl of protein G magnetic beads (+).

Lysate of cross-linked HEK293T cells was immunoprecipitated by an anti-β-catenin antibody or mouse IgG.

After 24 h, cells were incubated with or without PP2, lysed, and immunoprecipitated with anti-HA (left) or anti-myc (right) or control Abs (ctr Ab) followed by Western blotting with the indicated Abs.

METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.

When an antibody to endogenous SUV39H1 was used for immunoprecipitation, MeCP2 was effectively coimmunoprecipitated; conversely, aHA antibodies to HA - tagged MeCP2 could immunoprecipitate SUV39H1 (Fig. 2G).»