Sentences with phrase «immunoprecipitation analysis»

Genome - Wide Methylated DNA Immunoprecipitation Analysis of Patients with Polycystic Ovary Syndrome Hao - ran Shen, 1 Li - hua Qiu, 1 Zhi - qing Zhang, 2 Yuan - yuan Qin, 1 Cong Cao, 2, * andWen Di1, *

For Western blot assay and immunoprecipitation analysis, CD4 + T cells were isolated from mice spleen on day 21 after transplantation using a Mouse CD4 + T Cell Isolation Kit (Miltenyi Biotec), and the procedures were performed as described previously (35).

(G) Immunoprecipitation analysis of the acetylation of Akt.

Previous whole - genome chromatin immunoprecipitation analyses have identified a wide set of Oct4 target genes [6], [28], as well as describing co-occupancy of Oct4 and other transcription factors at many of these genes [6].

Not exact matches

Chromatin immunoprecipitation sequencing analysis revealed enrichment of H3K27me3 at specific loci in KDM6A - null cells, including PRC2 / EZH2 and their downstream targets.

Services include global and gene - specific DNA methylation analysis, and chromatin immunoprecipitation (ChIP) followed by library preparation in a high - throughput format (HT ChIP - Seq).

(e, g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells.

Importantly, this is one of the first reports applying Aβ immunoprecipitation followed by MALDI - MS analysis in CSF from a transgenic AD model, providing a robust, novel platform for the assessment of Aβ expression profiles in biological fluids of transgenic models.

To evaluate whether loss of ABL kinases affected TAZ activity, we performed chromatin immunoprecipitation (ChIP) analysis using primers for TAZ targets identified by ChIP sequencing analysis (42).

Chromatin immunoprecipitation and chip analyses revealed that both active and silenced genes in ES cells are directly bound by one or more of these three proteins [17], [19].

Here we present fCCAC, an application of functional canonical correlation analysis to assess covariance of nucleic acid sequencing datasets such as chromatin immunoprecipitation followed by deep sequencing (ChIP - seq).

(D) Treatment with 1 mM 8 - Br - cAMP for four hours enhances Oct4 binding to genomic loci as determined by chromatin immunoprecipitation, followed by real - time PCR analysis at Nanog and fgf12 sites.

A detailed description of the methods and analyses of methylated DNA immunoprecipitation (MeDIP) and microarrays hybridization used in this study were previously described [43] and can be found in File S1.