Sentences with phrase «immunoprecipitation as»

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Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

By using immunoprecipitation and mass spectrometry, we identified autoantibodies anti-Adenylate kinase 5 (AK5 - Abs) as specific marker in 10 patients with LE.

For Western blot assay and immunoprecipitation analysis, CD4 + T cells were isolated from mice spleen on day 21 after transplantation using a Mouse CD4 + T Cell Isolation Kit (Miltenyi Biotec), and the procedures were performed as described previously (35).

A diverse portfolio of high - quality primary and secondary antibodies is available for use in cancer research, epigenetics, immunology, neuroscience, and stem cell research as well as validated for multiple applications, including flow cytometry, western blotting, and immunoprecipitation.

His group uses high - throughput genomic methods — including DNA sequencing, genotyping, chromatin immunoprecipitation, mRNA expression profiling, transcriptional promoter and DNA methylation measurements — as well as computational and statistical tools to identify, characterize and understand the functional elements encoded in our genomes and how they work together at the molecular level in normal and pathological conditions.

Here we present fCCAC, an application of functional canonical correlation analysis to assess covariance of nucleic acid sequencing datasets such as chromatin immunoprecipitation followed by deep sequencing (ChIP - seq).

We present here the interactome of Nbeal2 with additional validation by reverse immunoprecipitation of Dock7, Sec16a and Vac14 as interactors of Nbeal2.

(B) Validation of fgf12 and PDE3A as bona fide Oct4 targets by chromatin immunoprecipitation with either serum (s, control) or monoclonal (1) or polyclonal (2) antibodies against Oct4.

Previous whole - genome chromatin immunoprecipitation analyses have identified a wide set of Oct4 target genes [6], [28], as well as describing co-occupancy of Oct4 and other transcription factors at many of these genes [6].

(D) Treatment with 1 mM 8 - Br - cAMP for four hours enhances Oct4 binding to genomic loci as determined by chromatin immunoprecipitation, followed by real - time PCR analysis at Nanog and fgf12 sites.

Chromatin immunoprecipitation was performed as previously described (Lee et al., 2006).

Quantitative serum AChR antibody concentrations were determined as previously described using an immunoprecipitation radioimmunoassay procedure [22].