Sentences with phrase «immunoprecipitation assays»

STAT6 co-immunoprecipitated with the transcriptional co-activator, p300, and chromatin immunoprecipitation assays demonstrated that these proteins bind a consensus STAT6 binding site located within the COX - 2 promoter.

Hanner and Rusche used chromatin immunoprecipitation assays to show that this chimeric protein did not make it to the HMRa or HMRα loci or to other repressed genes.

BRCTx binds to the C terminus of hRAD18 in yeast two - hybrid and immunoprecipitation assays and colocalizes with this protein in the nucleus.

Chromatin immunoprecipitation assay.

Not exact matches

Also works in immunoprecipitation of transfected cells, but reacts poorly in WB assays.

For Western blot assay and immunoprecipitation analysis, CD4 + T cells were isolated from mice spleen on day 21 after transplantation using a Mouse CD4 + T Cell Isolation Kit (Miltenyi Biotec), and the procedures were performed as described previously (35).

We confirmed that these compounds modulate Bach1 directly using chromatin immunoprecipitation and reporter assays.

To investigate whether unphosphorylated STAT6 binds to this site, chromatin immunoprecipitation (ChIP) assays using anti-STAT6 antibody were performed and demonstrated that unphosphorylated STAT6 bound to this consensus site (Figure 5a).

Chromatin immunoprecipitation (ChIP) assay was performed using EZ ChIP kit (Millipore) according to the instruction of the manufacturer.

Conducted cell based assays and techniques including SDS - PAGE, ELISA and immunoprecipitation.