Sentences with phrase «immunoprecipitation experiments»

For example, the 2015 Nature scientific report, Important biological information uncovered in previously unaligned reads from chromatin immunoprecipitation experiments (ChIP - Seq) discusses how various computational tools can find new information that can assist in the construction of gene regulatory grids.

Not exact matches

For the coimmunoprecipitation experiments, HuH - 7 cells transfected with pcDNA - SRα / FLAG - Sp 1 were harvested and cell lysates were prepared using immunoprecipitation lysis buffer [20 mmol / L Tris - Cl (pH 7.5), 150 mmol / L NaCl, 10 % glycerol, and 1 % Triton X-100].