Sentences with phrase «immunoprecipitation of»

We present here the interactome of Nbeal2 with additional validation by reverse immunoprecipitation of Dock7, Sec16a and Vac14 as interactors of Nbeal2.

Normal rabbit IgG (Millipore) was used to control the nonspecific immunoprecipitation of chromatin by immunoglobulins.

Also works in immunoprecipitation of transfected cells, but reacts poorly in WB assays.

Not exact matches

To measure bits of amyloid coursing through the bloodstream, Yanagisawa and colleagues used a technique called immunoprecipitation with mass spectrometry, which deploysantibodies to bind and identify proteins.

Chromatin immunoprecipitation sequencing analysis revealed enrichment of H3K27me3 at specific loci in KDM6A - null cells, including PRC2 / EZH2 and their downstream targets.

At ABclonal, we have nearly 100 existing chromatin immunoprecipitation (ChIP)- validated antibodies with a higher specificity than most commercial antibodies, which are used for a wide range of epigenetics studies, from those investigating developmental processes to those researching various diseases.

Immunoprecipitation with antibodies against dimethyl - K4 H3 was limited to the edges of the contig flanking the dimethyl - K9 H3 region [6].

For example, the 2015 Nature scientific report, Important biological information uncovered in previously unaligned reads from chromatin immunoprecipitation experiments (ChIP - Seq) discusses how various computational tools can find new information that can assist in the construction of gene regulatory grids.

(e, g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells.

BRCTx binds to the C terminus of hRAD18 in yeast two - hybrid and immunoprecipitation assays and colocalizes with this protein in the nucleus.

Importantly, this is one of the first reports applying Aβ immunoprecipitation followed by MALDI - MS analysis in CSF from a transgenic AD model, providing a robust, novel platform for the assessment of Aβ expression profiles in biological fluids of transgenic models.

Chromatin immunoprecipitation verified that HPP - 4382 treatment of NHLF cells reciprocally coordinated a decrease in binding of Bach1 and an increase of Nrf2 binding to the HMOX1 E2 enhancer.

To evaluate whether loss of ABL kinases affected TAZ activity, we performed chromatin immunoprecipitation (ChIP) analysis using primers for TAZ targets identified by ChIP sequencing analysis (42).

(G) Immunoprecipitation analysis of the acetylation of Akt.

We will map the mRNA interactome of DDX6 and mutant variants using protein - RNA crosslinking, immunoprecipitation, and next - generation sequencing to identify mRNAs where DDX6 binds, the region within the mRNA, and how the repertoire of putative targets changes.

A diverse portfolio of high - quality primary and secondary antibodies is available for use in cancer research, epigenetics, immunology, neuroscience, and stem cell research as well as validated for multiple applications, including flow cytometry, western blotting, and immunoprecipitation.

Enter HITS - CLIP, a new approach that applies high throughput sequencing of RNAs isolated by crosslinking immunoprecipitation.

Chromatin immunoprecipitation (ChIP) assay was performed using EZ ChIP kit (Millipore) according to the instruction of the manufacturer.

Furthermore, genome - wide studies, combining chromatin immunoprecipitation (ChIP) and array hybridization (ChIP - on - chip), have revealed that both active and silenced genes are directly bound in ES cells by one or more of the core pluripotency factors Oct4, Sox2 and Nanog [17], [19], [63].

Chromatin immunoprecipitation and chip analyses revealed that both active and silenced genes in ES cells are directly bound by one or more of these three proteins [17], [19].

Furthermore, by chromatin immunoprecipitation and whole - genome scanning approaches, we show that the chromosome ends of senescent cells directly contribute to the DNA damage response, and that uncapped telomeres directly associate with many, but not all, DNA damage response proteins.

Binding of Oct4 to several predicted PORE targets was verified via chromatin immunoprecipitation (ChIP, Figure 3B).

Here we present fCCAC, an application of functional canonical correlation analysis to assess covariance of nucleic acid sequencing datasets such as chromatin immunoprecipitation followed by deep sequencing (ChIP - seq).

June 4, 2015 New tool brings standards to epigenetic studies One of the most widely used tools in epigenetics research — the study of how DNA packaging affects gene expression — is chromatin immunoprecipitation (ChIP), a technique that allows researchers to examine interactions between specific proteins and genomic regions.

(B) Validation of fgf12 and PDE3A as bona fide Oct4 targets by chromatin immunoprecipitation with either serum (s, control) or monoclonal (1) or polyclonal (2) antibodies against Oct4.

Previous whole - genome chromatin immunoprecipitation analyses have identified a wide set of Oct4 target genes [6], [28], as well as describing co-occupancy of Oct4 and other transcription factors at many of these genes [6].

Genome - Wide Methylated DNA Immunoprecipitation Analysis of Patients with Polycystic Ovary Syndrome Hao - ran Shen, 1 Li - hua Qiu, 1 Zhi - qing Zhang, 2 Yuan - yuan Qin, 1 Cong Cao, 2, * andWen Di1, *

A detailed description of the methods and analyses of methylated DNA immunoprecipitation (MeDIP) and microarrays hybridization used in this study were previously described [43] and can be found in File S1.