Sentences with phrase «immunoprecipitation sequencing»

Chromatin immunoprecipitation sequencing analysis revealed enrichment of H3K27me3 at specific loci in KDM6A - null cells, including PRC2 / EZH2 and their downstream targets.

Not exact matches

Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

One current method commonly used today is called chromatin immunoprecipitation, combined with next - generation gene sequencing (ChIP - seq).

«fCLIP - seq stands for formaldehyde crosslinking, immunoprecipitation, and sequencing.

To evaluate whether loss of ABL kinases affected TAZ activity, we performed chromatin immunoprecipitation (ChIP) analysis using primers for TAZ targets identified by ChIP sequencing analysis (42).

This unique technology was launched in December 2017, and integrates automated chromatin immunoprecipitation and next generation sequencing library preparation within the same protocol, eliminating the traditional tedious steps, purifications, and inefficiencies that can result from standard ChIP - seq protocols.

We will map the mRNA interactome of DDX6 and mutant variants using protein - RNA crosslinking, immunoprecipitation, and next - generation sequencing to identify mRNAs where DDX6 binds, the region within the mRNA, and how the repertoire of putative targets changes.

His group uses high - throughput genomic methods — including DNA sequencing, genotyping, chromatin immunoprecipitation, mRNA expression profiling, transcriptional promoter and DNA methylation measurements — as well as computational and statistical tools to identify, characterize and understand the functional elements encoded in our genomes and how they work together at the molecular level in normal and pathological conditions.

Enter HITS - CLIP, a new approach that applies high throughput sequencing of RNAs isolated by crosslinking immunoprecipitation.

Here we present fCCAC, an application of functional canonical correlation analysis to assess covariance of nucleic acid sequencing datasets such as chromatin immunoprecipitation followed by deep sequencing (ChIP - seq).