(B) Validation of fgf12 and PDE3A as bona fide Oct4 targets by chromatin
immunoprecipitation with either serum (s, control) or monoclonal (1) or polyclonal (2) antibodies against Oct4.
Immunoprecipitation with antibodies against dimethyl - K4 H3 was limited to the edges of the contig flanking the dimethyl - K9 H3 region [6].
To measure bits of amyloid coursing through the bloodstream, Yanagisawa and colleagues used a technique called
immunoprecipitation with mass spectrometry, which deploysantibodies to bind and identify proteins.
Not exact matches
One current method commonly used today is called chromatin
immunoprecipitation, combined
with next - generation gene sequencing (ChIP - seq).
At ABclonal, we have nearly 100 existing chromatin
immunoprecipitation (ChIP)- validated antibodies
with a higher specificity than most commercial antibodies, which are used for a wide range of epigenetics studies, from those investigating developmental processes to those researching various diseases.
To this end, using knockout cells in combination
with siRNA and
immunoprecipitation approaches, our results revealed that Pen - 2 together
with presenilin are sufficient... to form a functionally active enzyme to process Notch.
(e, g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2
immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation
with recombinant human (rh) Ang1 compared to unstimulated (us) cells.
After extensive washing
with immunoprecipitation lysis buffer, the immunoprecipitated proteins were analyzed by immunoblotting using specific antibodies against DDX3 and FLAG epitope (ab1257, Abcam, Cambridge, United Kingdom).
BRCTx binds to the C terminus of hRAD18 in yeast two - hybrid and
immunoprecipitation assays and colocalizes
with this protein in the nucleus.
For the coimmunoprecipitation experiments, HuH - 7 cells transfected
with pcDNA - SRα / FLAG - Sp 1 were harvested and cell lysates were prepared using
immunoprecipitation lysis buffer [20 mmol / L Tris - Cl (pH 7.5), 150 mmol / L NaCl, 10 % glycerol, and 1 % Triton X-100].
By using
immunoprecipitation and mass spectrometry, we identified autoantibodies anti-Adenylate kinase 5 (AK5 - Abs) as specific marker in 10 patients
with LE.
(D) Cells transfected
with either Keap1 or control siRNA were subjected to chromatin
immunoprecipitation after treatment
with either 10 µM HPP - 1014, 10 µM CoPP, or 1 µM HPP - 4382 for 6 hours.
STAT6 co-immunoprecipitated
with the transcriptional co-activator, p300, and chromatin
immunoprecipitation assays demonstrated that these proteins bind a consensus STAT6 binding site located within the COX - 2 promoter.
Furthermore, by chromatin
immunoprecipitation and whole - genome scanning approaches, we show that the chromosome ends of senescent cells directly contribute to the DNA damage response, and that uncapped telomeres directly associate
with many, but not all, DNA damage response proteins.
We present here the interactome of Nbeal2
with additional validation by reverse
immunoprecipitation of Dock7, Sec16a and Vac14 as interactors of Nbeal2.
(D) Treatment
with 1 mM 8 - Br - cAMP for four hours enhances Oct4 binding to genomic loci as determined by chromatin
immunoprecipitation, followed by real - time PCR analysis at Nanog and fgf12 sites.
Genome - Wide Methylated DNA
Immunoprecipitation Analysis of Patients
with Polycystic Ovary Syndrome Hao - ran Shen, 1 Li - hua Qiu, 1 Zhi - qing Zhang, 2 Yuan - yuan Qin, 1 Cong Cao, 2, * andWen Di1, *