Sentences with phrase «immunoreactivity of»

By using sera from malaria - exposed immune adults, the immunoreactivity of 20 recombinant proteins was assessed, and most of the merozoite ligands were found to carry heat - labile epitopes.

The reduced immunoreactivity of immune sera to heat - denatured antigens (blue bar) demonstrates the presence of heat - labile (conformational) epitopes.

B, Histograms of GFP and P2X3 normalized immunoreactivity of individual cells showing that whereas the P2X3 staining shows a unimodal normal distribution (mean 0.58), the GFP population is bimodal suggestive of high - expressing (mean = 0.69) and low - or nonexpressing populations (mean 0.12).

Not exact matches

The most recent study report described in these same regions decreased tissue levels of 5 - HT and tryptophan hydroxylase, the synthesizing enzyme for serotonin, and no evidence of excessive serotonin degradation as assessed by levels of 5 - hydroxyindoleacetic acid (the main metabolite of serotonin) or ratios of 5 - hydroxyindoleacetic acid to serotonin.30 A recent article described a significant association between a decrease in medullary 5 - HT1A receptor immunoreactivity and specific SIDS risk factors, including tobacco smoking.40 These data confirm results from earlier studies in humans39, 41 and are also consistent with studies in piglets that revealed that postnatal exposure to nicotine decreases medullary 5 - HT1A receptor immunoreactivity.42 Animal studies have revealed that serotonergic neurons located in the medullary raphe and adjacent paragigantocellularis lateralis play important roles in many autonomic functions including the control of respiration, blood pressure, heart rate, thermoregulation, sleep and arousal, and upper airway patency.

MMP - 3 immunoreactivity was detected in cells within the lesion site, invading neutrophils, and blood vessel endothelial cells in the area outside of the initial injured area (the penumbra).

These GFP - labeled fibers were, however, only a subset of the total gustatory innervation of the taste buds, as revealed by immunoreactivity for P2X3 (Fig. 3E).

Regions of interest in the perikaryon of individual ganglion cells (as identified by P2X3 immunoreactivity) were outlined using ImageJ (RRID: nif -0000-30467).

GFP immunoreactivity was detected in a subset of geniculate ganglion neurons (169/682, 24.8 %; Fig. 2B, C), whereas the P2X3 signal was present in nearly all ganglion cells (664/682, 97.4 %; Fig. 2B, C).

(H) Cells expressing the rod photoreceptor marker XAP2 (rPR; red); BrdU - immunoreactivity (yellow) identifies mitotically active cells in the periphery of the same flank retina.

BrdU immunoreactivity is not conclusive evidence for the presence of retinal stem cells.

All muscle fibers exhibit complex II immunoreactivity, consistent with the nuclear DNA - encoded expression pattern of this and all other subunits of complex II.

Quantification of GFAP and Iba1 immunoreactivity revealed parallels in both mouse models in addition to distinct patterns of microglial activation.

Relationship of cannabinoid CB1 receptor and cholecystokinin immunoreactivity in monkey dorsolateral prefrontal cortex.

Isoform - dependent effects of apoE on doublecortin - positive cells and microtubule - associated protein 2 immunoreactivity following 137 Cs irradiation.

Ottersen OP, Storm - Mathisen J, Somogyi P (1988) Colocalization of glycine - like and GABA - like immunoreactivities in Golgi cell terminals in the rat cerebellum: a postembedding light and electron microscopic study Brain Res, 450 (1 - 2), 342 - 53 PubMed 2456823

Furthermore, in brains from APP transgenic mice conformation - specific antibodies have revealed the early appearance of intraneuronal fibrillar and oligomeric Aβ immunoreactivity, which declined as amyloid plaques appeared, and further became evident in the extracellular space [10].

Note the absence of McSA1 - immunoreactivity in non-transgenic animals (m).

The examination of the amyloid pathology with super-resolution microscopy permitted us to further reveal a clear separation of the subcellular location of Aβ - immunoreactivity from that of APP / CTFs.

Bicker G, Schäfer S, Ottersen OP, Storm - Mathisen J (1988) Glutamate - like immunoreactivity in identified neuronal populations of insect nervous systems J Neurosci, 8 (6), 2108 - 22 PubMed 2898517

Catecholaminergic neurons containing GABA - like and / or glutamic acid decarboxylase - like immunoreactivities in various brain regions of the rat

Note the distinct intracellular localization of McSA1 immunoreactivity (green) with that of pab27576 (red), denoting Aβ - amyloid peptides and APP / CTFs, respectively.

Our microscopy studies at high magnification and high resolution have revealed a clear segregation between Aβ - immunoreactivity and that of APP and its C - terminal fragments (CTFs).

Characterization of McSA1 and pab27576 immunoreactivities by super-resolution microscopy.

Pre-adsorption of this antibody with synthetic Aβ42 completely abolished McSA1 immunoreactivity.

Corroborating our previous results with McSA1, we observed a differential pattern of immunoreactivity between MOAB - 2 and pab27576 in cells with detectable and reliable MOAB - 2 staining.

Note the absence of pab27576 immunoreactivity in amyloid plaques (a-c).

The cellular and subcellular location of the immunoreactivity is defined and a summarizing text is provided describing the general staining pattern.

The uptake and degradation of fibrils was analysed by quantitative light - and electron - microscopic immunocytochemistry and immunoreactivity was detectable in 86 + / -3 % cells within 6 h of culture.

Effects of neonatal treatment with valproic acid on vasopressin immunoreactivity and olfactory behaviour in mice.

The reduction in CD68 immunoreactivity between sedentary and exercised Grn − / − mice is consistent with the general anti-inflammatory effects of exercise, including those found in other mouse models of neurodegenerative disease (Gleeson et al., 2011; Stranahan et al., 2012).

We assessed microgliosis by immunostaining for CD68 and found significantly elevated CD68 immunoreactivity in the frontal cortex, hippocampus, and thalamus of Grn − / − mice, as expected (Fig. 6A w - y).

For 16 of the 20 proteins (all but MSRP4, SERA7, PFA0210c, and PFB0475c) the immunoreactivity decreased significantly when the proteins were heat inactivated, establishing that the antigens contain heat - labile epitopes.

B: Double - labeling of ZENK and the retrograde tracer BDA in sagittal brain sections at the level of Cluster N proves the correct placement of tracer into Cluster N: arrows point to examples of neurons displaying ZENK - immunoreactivity (shown in magenta) in the nucleus together with BDA (shown in green) in the somata.

ZENK protein immunoreactivity displayed a similar pattern as previously shown for the expression of ZENK mRNA [Fig. 3; (13)-RSB-.

Our quantitative assessment of doublecortin (DCX) immunoreactivity in the dentate gyrus showed a loss of DCX in aged animals (Supplemental Figure 1), consistent with a substantial decrease in the neurogenic capacity of the aged hippocampus.

Iodination of human thyroglobulin (Tg) alters its immunoreactivity.

These limitations have led to the development of assays for enzymes or markers considered pancreatic in origin such as trypsin - like immunoreactivity (TLI), trypsinogen activation peptide (TAP), and pancreatic lipase immunoreactivity (PLI).

Serum trypsin - like immunoreactivity, amylase and lipase in the diagnosis of feline acute pancreatitis.

Additional procedures such as ultrasonography, abdominal paracentesis or assay of trypsin - like immunoreactivity, TAP or immunoreactive canine pancreatic lipase are usually performed on the basis of these initial test results and help to distinguish pancreatitis from other intra-abdominal causes of vomiting.

Levels of total alpha - macroglobulin and trypsin - like immunoreactivity are poor indicators of clinical severity in spontaneous canine acute pancreatitis.

Recently, assays for measurement of pancreatic lipase immunoreactivity in dogs and cats (cPLI and fPLI, respectively) have been developed and validated.

The initial evaluation (A) comprises obtaining a thorough case history (A1), conducting a physical examination (A2), and taking the basic laboratory tests, including a complete blood count, a serum chemistry profile, and measurement of serum concentrations of trypsin - like immunoreactivity (TLI)(A3).

Trypsin - like immunoreactivity (TLI) tests are the most specific of the lab tests, but changes are not always present in affected cats.

Those obstacles led to the development of the pancreatic lipase immunoreactivity test (PLI) and trypsin - like immunoreactivity tests.

B12 supplementation: Since more than four out of five dogs with exocrine pancreatic insufficiency are estimated to have insufficient levels of vitamin B12, it's a good idea for the vet to test blood levels of that nutrient at the same time she orders a trypsin - like immunoreactivity blood test.

The trypsin - like immunoreactivity test (TLI) is the most common diagnostic tool used and requires only one sample of blood taken after a 12 - 18 hour fast.

The diagnosis is confirmed by demonstrating a subnormal serum concentration of trypsin - like immunoreactivity (TLI), recently reviewed by Westermarck & Wiberg (2003).

Interpretation of tyrosin - like immunoreactivity (TLI) values in fasting dogs.

Forman M. Evaluation of Feline Pancreatic Lipase Immunoreactivity and Helical CT versus Conventional Testing for the Diagnosis of Feline Pancreatitis.

After ruling out other possible causes of diarrhea, a diagnosis of EPI is confirmed with a blood test called a trypsin - like immunoreactivity test, or TLI.