Sentences with phrase «immunostaining of»

Therefore, immunostaining of this molecule was used to visualize vasculature in heterochronic old (Het - O) and isochronic old (Iso - O) mice.

(D) Immunostaining of H3K4me3 and H3K18ac in tumor samples of PTCL - NOS patients with or without KMT2D or EP300 mutations.

(G) Immunostaining of p - ERK in tumor samples of xenografted murine models bearing KMT2D V5486 mutants treated with CHID and / or DECI.

Age, tumor size, tumor site, LNM, VI and HER2 / neu immunopositive stain as well as the immunostaining of ER and PR for the malignant group were obtained from the Pathology Department, MRI.

They used exome sequencing and immunostaining of DNA damage response proteins in order to evaluate HRR, and findings were then validated in a cohort of breast cancer patients with BRCA1 / 2 mutations who were treated with PARP inhibitors.

Not exact matches

Using immunostaining, the researchers counted the remaining tumor cells; the mice treated with GD2 CAR - T cells had a few dozen remaining cancer cells per animal, while each control mouse had tens of thousands of cancer cells.

«Immunostaining conducted with skin samples collected from dogs with the disorder proved that the functional form of the protein, or collagen, was absent from the skin of these dogs,» says Kati Dillard, a veterinarian at the Finnish Food Safety Authority Evira.

A rare - stem like tumor cell, which plays a critical role in the spread of breast cancer, is identified with immunostaining for the?

Professor Zhang's team were able to visualize the pinning, stretching and rupturing of cervical cancer cells by immunostaining different parts of the cells and viewing them under a confocal fluorescent microscope and a high - resolution scanning electron microscope (SEM).

Representative images of P - PERK (green) in B - cells (red) immunostained for insulin in young undisturbed (YC), young SD (YSD), old undisturbed (OC) and old sleep deprived (OSD) mice.

To achieve this goal, her lab has developed technology platforms for large - scale immunostaining and systematic validation of antibody specificity, and has initiated citizen - science efforts to refine the Human Protein Atlas database.

Previously, kinetic analysis of GLUT4 was performed by using fluorescence proteins and an immunostaining method; however, it was impossible to precisely examine if GLUT4 in the cell had been transiently translocated to the cell membrane.

The distribution and morphology of neurons and axonal endings (puncta) immunostained with antibodies to gamma - aminobutyric acid (GABA) and glycine (Gly) were analyzed in auditory brainstem, thalamic,

Quantification of HNF4α immunostainings showed that over 90 % of hiPS cell - derived hepatocytes differentiated using our protocol express HNF4α [4].

HNF4α immunostaining showed over 90 % of hepatocytes differentiated with our protocol expressed HNF4α (Asplund et al. 2016).

Furthermore, there was a negative expression for Cyclin D1 protein in normal ductal epithelial cells, while the benign group revealed week positivity immunostaining Cyclin D1 in 60 %, whereas, the malignant group represented a variable positivity of immunostaining scores; they were classified as 3 (10 %) cases were weak, 9 (30 %) moderate cases and 12 (40 %) strong expression cases.

(A) Normal case: negative Cyclin D1 - immunostaining in the ductal epithelial cells, (B) Benign case: few nuclei with weak expression of Cyclin D1 of ductal epithelial cell, and negative one in stromal cells, (C) IDC GII case: a strong positive expression of Cyclin D1 in invasive ductal epithelial cells and stroma, (D) IDC GIII case: moderate positive expression of Cyclin D1 in trabeculae malignant cells, and absent in the vesiculated polymorphic nuclei.

According to the immunostaining results of hormonal status noticed 66.7 % of GII and GIII cases expressed positive ER immunostaining.

Persistence of B. burgdorferi was evaluated using xenodiagnosis, bioassays in mice, multiple methods of molecular detection, immunostaining with polyclonal and monoclonal antibodies and an in vivo culture system.

They then successfully generated custom antibodies to each of these proteins and confirmed that there was no cross-reactivity, opening the door for immunostaining analysis.

During sperm biogenesis, flagella appear tightly bundled, which makes it more challenging to get accurate measurements of protein levels from immunostained specimens.

Interestingly, RIPK2 has been demonstrated to be involved in the active growth of CD90 (+) intestinal stromal cells to suggest an «inflammatory» cross-talk between intestinal stromal cells and the epithelial cells (Owens et al., 2013), and we can observe robust activation of RIPK2 in IBD colon tissue sections immunostained with our proprietary pY474 RIPK2 antibody (Salla et al., unpublished observations).

Slides were imaged on an upright microscope (Nikon), and two images per brain region, per mouse were analyzed for density of GFAP or CD68 immunostaining or for the number of doublecortin - positive neurons.

The sections of day 14 EBs were doubly immunostained with anti-SSEA1 and anti-OCT-4 (C — H) antibodies, or anti-OCT-4 and anti-VASA (I — N) antibodies.

Sections of 3 - year - old (D, E) and 5 - year old testes (F — Q) were examined by immunostaining.

Analysis of CD68 immunostaining in the frontal cortex revealed an effect of genotype (ANOVA, p < 0.0001) and a strong trend for an exercise effect (ANOVA exercise effect, p = 0.051).

We assessed microgliosis by immunostaining for CD68 and found significantly elevated CD68 immunoreactivity in the frontal cortex, hippocampus, and thalamus of Grn − / − mice, as expected (Fig. 6A w - y).

To determine if there was any dopaminergic terminal degeneration in any of our mice, we immunostained sections through their striata for TH and quantified the stain intensity using an infrared imaging system.

(D) Sections of EBs grown under the same conditions as in part (C), showing that the outer layer consists of visceral endoderm as marked by Gata6, FoxA2, and Sox17 immunostaining (shown as red).

The presence of SSEA1 expressing cells in EBs was determined by immunostaining.

This may reflect the mixed population of germ cells in the EBs, which was demonstrated by the immunostaining analysis.

B, Representative images (20 ×) of CD68 immunostaining from the frontal cortex.

Epidermal stem cells from mouse tail skin visualized by wholemount 3D immunostaining: Left panel shows epidermal stem cells marked with BrDU (Label Retaining Cells); Middle and right panels Show expression of keratin - 15 and high expression of integrin alpha6, respectively.

Representative photomicrographs of TH - immunostained E14 rat VM DA neurons immunostained for BMPR2 (A, B) or Smad1 / 5 (C, D).

Methods: We performed immunostaining to assess the localization of PRCP in arcuate Neuropeptide Y / Agouti - related Peptide (NPY / AgRP) neurons.

A surprising finding of our newly constructed UAS - DTH1m is that it expresses DTH at an undetectable level when regulated by dopaminergic GAL4 drivers even when fully rescuing DA, indicating that DTH immunostaining is not necessarily a valid marker for DA expression.

Image shows strong immunopositivity at the membranes of cardiomyocytes (invaginations of immunostaining show points of branching of each myocyte).

(C and D) Average lesion number (C) and area (D) were quantified using images of immunostaining for RCAS - caErbb2 - HA.