In telomeric DNA mutants of Tetrahymena thermophila, created by expression of a telomerase RNA with an altered template sequence, division of the germline nucleus was severely delayed or blocked
in anaphase.
Not exact matches
In the majority of cells arrested after S phase but before
anaphase, sister minichromosome molecules are not topologically interlocked with each other.
The role of DNA topology
in holding sister chromatids together before
anaphase was investigated by analyzing the structure of a small circular minichromosome
in cell cycle (cdc) mutants of the yeast Saccharomyces cerevisiae.
The close temporal association of the [Ca2 +] i transient with the onset of
anaphase suggests that calcium may have a signaling role
in this event.
Prior studies by Kerr el al, showed that PP2ACdc55 played an essential role
in controlling the timing of metaphase to
anaphase during meiosis,
in other words preventing cells from prematurely exiting meiosis.
Despite this hard work from dynein,
in roughly one - quarter of the cells observed, the spindle was misaligned, even after the paired chromosomes were pulled to opposite sides of the cell
in late
anaphase.
In approximately 43 % of the dividing human cells Kiyomitsu observed, the middle of the mitotic spindle was properly centered at the beginning of
anaphase.
Kiyomitsu's latest work focuses on the next step of mitosis, called
anaphase, when the microtubules tear the paired chromosomes apart so that one copy of each chromosome ends up
in each of the new daughter cells.
As seen
in this merged fluorescent image (early
anaphase marked with magenta; late
anaphase marked with green), such cells correct this problem by adjusting their cell membranes relative to the spindle (right), instead of shifting their spindles within the cell.
Kim, Jung - Sik, et al. «Intact cohesion,
anaphase, and chromosome segregation
in human cells harboring tumor - derived mutations
in STAG2.»
Martin Schwickart (Zachariae, MPG)-- «Molecular analysis of novel subunits of the
anaphase - promoting complex (APC / C)
in yeast» (2005)
The END network couples spindle pole assembly to inhibition of the
anaphase - promoting complex / cyclosome
in early mitosis.
DNA damage leads to a Cyclin A — dependent delay
in metaphase -
anaphase transition
in the < i > Drosophila gastrula.
Mutual antagonism between the
anaphase promoting complex and the spindle assembly checkpoint contributes to mitotic timing
in Caenorhabditis elegans
Left: Heidi Norton,
Anaphase Mutated, 2014, glass, resin, plants, paint, wood, 61 1/2 x 52
in.