Antibodies to env, gag and pol are seen
in an immunoblot
In this webinar we will explore the issues of reproducibility
in immunoblotting in more depth and discuss how to obtain higher quality data, with a focus on those key factors necessary to create consistent, quantifiable results.
Reacts with p66 / p51
in immunoblots and inhibits the reaction of RT enzyme in a Biological assay.
Not exact matches
Protein
immunoblot and histochemical analysis with antiserum to type I NO synthase suggest that the formation of NO
in pancreatic B cells is catalyzed by an NADPH -(reduced form of nicotinamide adenine dinucleotide phosphate), Ca2 + / calmodulin - dependent type I NO synthase of about 150 kilodaltons.
Quantification of rapid Myosin regulatory light chain phosphorylation using high - throughput
in - cell Western assays: comparison to Western
immunoblots.
Western Blotting is an analytical
Immunoblotting Technique to detect specific proteins
in a cell extract or tissue homogenate.
Immunoblots showed almost complete inhibition of mTOR expression (as shown
in Fig. 4B) that resulted
in decreased cell proliferation as assessed by MTS assay (A).
Equal amounts of protein were solubilized
in 2.5 x SDS - sample buffer, separated on 12 % SDS - polyacrylamide gels, transferred to Immobulin P and
immunoblotted with the antibodies indicated
in the Materials section.
Immunoblots showed strong expression of HA tag
in infected ALCL cells but not
in control (uninfected or infected with adeno - β - Gal) cells (A).
Immunoblots showed a concentration - dependent decrease
in the antiapoptotic proteins BCL - 2, BCL - XL, MCL - 1, and c - FLIP (long and short) with increasing concentrations of rapamycin (C, left).
Immunoblot analysis using mouse monoclonal antibody to human IgM detected IgM to cytomegalovirus (CMV)- specific proteins (150, 42, 38, 32, and 28 kDa)
in 74 (38 %) of 197 seropositive serum samples from 197 individuals
in three subject groups: 43 surgical patients, 31 patients with solid tumors, and 123 healthy individuals.
(A)
Immunoblot illustration of the use of RIPK2 phospho - antibodies
in two Hodgkin's lymphoma cells that have constitutive active RIPK2.
2 days after release of UO or at the corresponding time point
in the non-UO sham - operated mice, the left kidneys were harvested and subjected to
immunoblot analyses of HIF - 2α and co-detection of TBP as a loading control