Sentences with phrase «in a culture dish with»

The colonies were fixed in culture dishes with 4 % paraformaldehyde in phosphate - buffered saline (0.01 M PBS, pH 7.4) for 20 min at room temperature (RT) followed by washing with PBS (2 × 5 min).

Not exact matches

Smørrebrød — an open - faced rye sandwich piled high with toppings — is one of the most essential dishes in the Danish culture, most commonly eaten for...

Everyone brings a dish to share so lunch is always a beloved mish - mash of cultures — last year my mum's soya sauce duck with Chinese mushrooms fought for space on the overloaded table with my Auntie R's traditional roast turkey, my sister in law's clove - studded ham, our friend's Malaysian lamb curry, king prawns, oysters, my ex's German coleslaw and potato salad, noodles, smoked fish and quinoa salad brought by friends and my dad's famous lobster noodles.

Discovering dishes from different cultures, exploring new flavor combinations, and simply stuffing her face on some homemade comfort food are definitely past times that make her top five — along with scribbling about them in her notebook.

In addition to the delicious day - to - day options, guests can indulge in Wednesday Ethnic Inspirations tasting menus, which feature dishes that celebrate numerous cultures of cuisine, as well as afternoon tea times with dessertIn addition to the delicious day - to - day options, guests can indulge in Wednesday Ethnic Inspirations tasting menus, which feature dishes that celebrate numerous cultures of cuisine, as well as afternoon tea times with dessertin Wednesday Ethnic Inspirations tasting menus, which feature dishes that celebrate numerous cultures of cuisine, as well as afternoon tea times with desserts.

Family meals are an important part of the day in any culture, but with today's busy lifestyles, we are more often grabbing things on the go and rushing to finish the meal with the minimum of dishes to wash up afterward.

A California native, Christina grew up in a culture that placed emphasis on dishes created around a diversity of fruits and vegetables with a wide variety of flavors.

U.S. Attorney Preet Bharara, in his typical machine - gun staccato delivery, pop culture references dished out with an arched eyebrow and declarations best suited for a James Ellroy noir, seemed more disappointed than angry at yesterday's news conference that he keeps having to police Albany.

But T. auensis is particularly hard to culture in Petri dishes and study with the normal tools of molecular biology.

In a culture dish microglia that were modified to make a lot of TREM2 gobbled more amyloid and removed more dying neurons, compared with microglia having less of the protein.

To grow these microorganism cultures in the lab, researchers house samples in petri dishes lined with a nourishing gel mixture derived from algae called agar growth media.

They studied H. pylori bacteria in a culture dish and compared the effect of only the antibiotic metronidazole against the bacteria versus the prodrug system with metronidazole and carbon monoxide combined.

When Stamets cultured the mold in his lab, a white circle of mycelium spread over the petri dish from the point of inoculation; it was soon covered with green spores.

Zheng, together with Leah Boyer, then a researcher in Gage's lab and now director of Salk's Stem Cell Core, generated diseased neurons by taking skin cells from patients with Leigh syndrome, reprogramming them into stem cells in culture and then coaxing them to develop into brain cells in a dish.

In cells grown on flat culture dishes, the expression of thousands of genes didn't match up with their normal patterns, explaining why the cells from those dishes had been unable to generate new hair follicles.

Rather than stick the isolated cells on a flat culture dish, they mixed the cells with liquid, then let the mixture hang in tiny droplets from a plastic lid, like condensation on the roof of a container.

«We culture typical skin cell of the epidermis, such as human keratinocytes, in our dishes to form an artificial epidermis with all of its natural layers,» explained Sibylle Thude, the biologist who led the investigation into the accreditation.

Studies of individual neurons in dishes of cultured neurons and in brain slices by co-first author Robert E. Stanley, a graduate student in the lab, revealed that neurons in the mutant mice had decreased numbers of dendritic spines, an important part of the synapses that neurons use to communicate with one another.

The investigators studied application of various doses of a common PBDE flame retardant on human adrenal cells in culture dishes and compared the effects with those of only the vehicle, the inactive substance used to deliver the chemical.

They then combined these in a dish with testicular cells from newborn mice in various culture conditions — a process that took hundreds of trials, says author Xiao - Yang Zhao, a stem cell biologist now at Southern Medical University in Guangzhou, China.

They have spread lies about his methods, accused him of electrocuting monkeys when in fact he was working with cells in culture dishes, and limited his job opportunities.

The research builds on the team's previous work with a technique called three - dimensional culture, which involves incubating stem cells in a floating ball - shaped aggregate, unlike traditional cell culture in which cells grow in a flat layer on the surface of a culture dish.

Normally, CD4 cells will start dividing when they are treated with certain proteins in a culture dish.

COS - 7 cells, plated at 70 % confluence in 100 - mm tissue culture dishes, were transfected with vector alone or pcDNA3 - ACVR1 (c. 617G) or pcDNA3 - ACVR1 (c. 617A).

In initial work with cell cultures in Petri dishes, the researchers methodically ruled out the possibility that HIV / gp120 would be inducing the death of stem cells and determined instead that HIV / gp120 was acting by inhibiting stem cell proliferatioIn initial work with cell cultures in Petri dishes, the researchers methodically ruled out the possibility that HIV / gp120 would be inducing the death of stem cells and determined instead that HIV / gp120 was acting by inhibiting stem cell proliferatioin Petri dishes, the researchers methodically ruled out the possibility that HIV / gp120 would be inducing the death of stem cells and determined instead that HIV / gp120 was acting by inhibiting stem cell proliferation.

Your culture dishes could be infected with Mycoplasma, tiny bacteria that thrive in cell culture environments.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Dissected brains were immersed in 200 μl of PBS and tethered with tungsten wire (0.125 mm diameter, A-M Systems, Inc.) to the bottom of a culture dish (35x10 mm, Falcon).

HepG2 cells in 100 mm cell culture dishes were transfected with pGL - MARE - Luc plasmid DNA along with plasmid carrying the human Bach1 gene or the empty vector pFLAG - CMV - 6c using Fugene6 (Promega).

The cells were enzymatically removed from the culture dish with Tryple ™ Select (Invitrogen) for 15 min at 37 °C, resuspended in 1 ml FACS buffer I (2 % FBS, 0.01 % sodium azide in PBS), and counted with Neubauer cell counter chambers.

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

Neuronal outgrowths in 24 - well tissue culture dishes were fixed, permeabilized and blocked as above prior to staining with anti-β-III tubulin, 1 ∶ 200 (Chemicon mab1637 followed by secondary labeling with AF488 goat anti-mouse IgG1 (A21121, Invitrogen).

SCR055, Millipore, Inc) in 24 - well culture dishes coated with poly - L - ornithine (20 µg / ml) and laminin (5 µg / ml).

Manipulating genes and then sorting through many cells to find the desired alterations wasn't possible with these cells because scientists couldn't grow them in the culture dishes where such maneuvers would take place.

This Korean braised short ribs recipe is one of the tastiest meat dishes in Korean culture with the perfect fall off the bone flavor.

Our passion is discovering culinary gems in the sprawling metropolis of Bangkok and our mission is to share them with you, dishing up culture, tradition and people behind the savory treats along the way.

In their quest to innovate, the Roca brothers, co-owners of El Celler De Can Roca (recently named the World's Best Restaurant) closed the restaurant for five weeks to tour the world with the entire staff, and design 57 new dishes paying tribute to local food traditions and ingredients and the power of global food culture.

Most people tend to equate Japanese cuisine with raw fish dishes such as sashimi or sushi but in reality Japan's culinary culture is far more extensive.

As with many immigrant populations, it's the food and drinks of the culture that has had the biggest impact: western Sydney in particular is dotted with restaurants serving traditional Lebanese banquets and signature dishes like fattoush and flatbreads.

Our passion is discovering culinary gems in the sprawling metropolis of Bangkok and our mission is to share them with you, dishing up culture, tradition and people behind the savory treats along the way.

Don't look at the culture you're visiting as an outsider, but inside dive in headfirst by interacting with locals, taking cultural classes, sampling typical dishes, attending community events and partaking in local life as much as possible.

After the visit to Chichen Itza delve into the mayan culture, you will taste typical dishes of the region in a mexican buffet restaurant, with option for vegetarians.

For those who are keen to immerse themselves in the Spanish culture we also offer accommodation with host families, which is a great way to practice Spanish with native speakers and try local homemade dishes.

This taste of Colombian culture goes a step further in the form of the amazing food served in the beautiful dining area, where they serve fresh, local seafood and other authentic dishes — and they also cater to those with vegan or vegetarian inclinations!

One thing that I think could be woven in is «practical art» — like pottery dishes with coordinating decorative pieces and that are very decorative by themselves; Specific «rite of passage» art and the meaning behind it, as Melissa's ketubah art; and I the American quilt is an art form, as is weavings and wood carving and fabric constructions across many cultures... perhaps the «niche» TAA would best fit is a person whose basic needs have been accomplished (like clean water, food, shelter) and they are ready to move into becoming self - sufficient through their artistic trade.

The exhibition features key works including Data Zone (2003), a group of long tables embedded with illuminated Petri dishes in which silent clusters of abstracted figures, which form and reform, evoke laboratory cultures.

Our passion is discovering culinary gems in the sprawling metropolis of Bangkok and our mission is to share them with you, dishing up culture, tradition and people behind the savory treats along the way.