Sentences with phrase «in adherent cells»

D, the expression of miR - 7 was examined in adherent cells and mammospheres derived from MCF7 - BoM cells by TaqMan PCR.

Not exact matches

The scientists then tried the proline / polyproline combination in a test system of adherent cell cultures that were subjected to freeze - thaw conditions.

In Vivo Adipogenesis in Rats Measured by Cell Kinetics in Adipocytes and Plastic - Adherent Stroma - Vascular Cells in Response to High - Fat Diet and ThiazolidinedionIn Vivo Adipogenesis in Rats Measured by Cell Kinetics in Adipocytes and Plastic - Adherent Stroma - Vascular Cells in Response to High - Fat Diet and Thiazolidinedionin Rats Measured by Cell Kinetics in Adipocytes and Plastic - Adherent Stroma - Vascular Cells in Response to High - Fat Diet and Thiazolidinedionin Adipocytes and Plastic - Adherent Stroma - Vascular Cells in Response to High - Fat Diet and Thiazolidinedionin Response to High - Fat Diet and Thiazolidinedione.

Limiting dilution tumor formation of parental, hypoxia - exposed adherent, and cycling hypoxia - selected breast cancer cells in vivo

Since human embryonic stem cells grow in an adherent culture system, for cells being reprogrammed this «new culture system» is an adherent culture system (to try and mimic the conditions the embryonic stem cells want to be happy).

In the late 1960s, Rowley and his graduate student Donald Mosier were the first to describe the function of a previously unrecognized cell type, a component of the adaptive immune system they labeled the «A cell,» for its «adherent, accessory or antigen - presenting capacity.»

Cells were detached (adherent culture) or dissociated (spherical culture) by trypsin / EDTA and resuspended in PBS for cell counting using the ViaCount assay.

In short, there is no blastocyst - like stage — when the somatic cells are reprogrammed, they're immediately cultured as cells in normal adherent culturIn short, there is no blastocyst - like stage — when the somatic cells are reprogrammed, they're immediately cultured as cells in normal adherent culturin normal adherent culture.

Bone marrow - derived mesenchymal stem cells (BM - MSCs) are obtained by isolating mononucleated cells from a marrow aspirate by centrifugation on a density gradient, followed by adherent cell expansion in tissue culture.

Furthermore, we found that the stem - like and EMT phenotypes observed in the cycling hypoxia - selected subpopulation are not reversible, because we obtained the same molecular profile from this subpopulation by culturing them as spherical cultures for several months in normal oxygen content and culturing them as adherent cells (Figure S2 in Additional file 2).

Viral Manufacturing To manufacture viral vectors, two approaches generally are adopted for culturing purposes: scale - out of adherent - cell systems based on 2D planar technologies and scale - up of 3D suspension cell cultures in stirred - tank bioreactors.

For large - scale manufacturing, the conventional laboratory processes of adherent - cell systems (e.g., T - flasks, roller bottles, hollow - fiber technologies, and multilayer flasks) pose considerable challenges in scaling up (4).

Cells were plated at 50 cells per sq. cm in adherent conditions for 10 Cells were plated at 50 cells per sq. cm in adherent conditions for 10 cells per sq. cm in adherent conditions for 10 days.

Adherent cells were washed once (PBS supplemented with 100 mg / L calcium chloride and 100 mg / L magnesium chloride), fixed (4 % paraformaldehyde, 10 min), then permeabilized (0.3 % Triton X-100 in PBS, 30 min).

Though monoclonal antibodies were a model product in his 18 years CMO experience, process development was also performed for enzymes, hormones, blood factors, immuno - modulants and vaccines with different expression systems such as hybridoma, myeloma, CHO, BHK, insect - cell / baculovirus, adherent animal and human cells.

FS's cadmium - telluride solar cells technology is definitely gaining adherents in both technical and commercial side of the industry.