Sentences with phrase «in agarose»

Treated cell suspensions were over-layered in agarose coated slides as described [19].

After cells held in suspension in an agarose solution are grown around the vascular structure, a solvent can be used to wash the sugar away.

The design differs from that of a conventional commercial microscope: Keller placed the laser and objective lens at right angles to each other atop a table; then he embedded zebrafish embryos in agarose in a sample tube between the two.

Not exact matches

He and his colleagues wanted to avoid the classic light - sheet arrangement in which the sample is embedded in a tube of agarose and surrounded by lasers and cameras.

Conditions for the PCR reaction were 40 cycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s. Amplicons were purified on a 2 % agarose gel, cloned into plasmid vectors using TOPO TA (Invitrogen, Carlsbad, CA), and sent to an outside company (Elim Biopharmaceuticals, Hayward, CA) for Sanger sequencing in both directions using vector primers M13F and M13R.

Amplified sequences were visualized by gel electrophoresis in 2 % agarose gels stained with GelRed (Biotium).

Clade B recombinant Nef protein, expressed as a His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.

Using chondroitin sulfate to improve the viability and biosynthesis of chondrocytes encapsulated in interpenetrating network (IPN) hydrogels of agarose and poly (ethylene glycol) diacrylate.

Clade C recombinant nef protein, expressed as His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.

Clade A recombinant nef - protein, expressed as His - tag fusion protein in E. Coli using expression vector pET24 and purified using Ni - agarose columns

Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].

Amplicons were visualized in a 2 % agarose gel stained with Ethidium Bromide and purification was performed directly from the amplification reaction using the Qiagen PCR purification Kit according to the manufacturer's instructions.

PCR products were visualized by electrophoresis in an ethidium bromide - stained 1.8 % agarose gel.

DNA libraries were then re-amplified for another 13 cycles in quintuplicates or sextuplicates, followed by pooling and purification, visual inspection on a 3.5 % agarose gel, and final quantification using a NanoDrop 2000c spectrophotometer (FisherScientific).

The resulting PCR amplification products were analyzed by electrophoresis in 1.5 % agarose gels.

At 24 hours post fertilization (hpf), fluorescent embryos were dechorionated, mounted on coverslips in 1.2 % ultra-low gelling agarose, overlaid with 30 % Danieua / PTU and analyzed by laser scanning confocal microscopy (Zeiss LSM510 Meta).

This product was similarly purified in a low - melt agarose gel and used at 1 ng / μl along with 50 ng / μl of the unc - 36 (+) cosmid derivative RIp16 (gift of L Lobel) for microinjection into unc - 36 -LRB--) animals.

PCR products were visualized by blue light after electrophoresis on a 2.5 % agarose gel containing 1 × GelStar ® Nucleic Acid Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.0).

Performed various tasks in the lab such as preparing agarose gels and buffer dilutions for electrophoresis.

Responsible and highly motivated professional with solid and diverse experience as Molecular Biologist with excellence in isolating and purifying DNA, bacterial plating, polyacrylamide gel electrophoresis, agarose gel electrophoresis.

The SLC6A4 promoter VNTR polymorphism was genotyped by agarose gel size fractionation as described in ref.